Isimiso

I-strip ine-anti cal coating MCAB kwisifunda sokuhlola kanye nembuzi anti-rabbit IGG antilody kwisifunda sokulawula, esifakwe ku-chromatography kusenesikhathi. I-Lable Pad ihlanganiswe ngegolide le-Colloidal legolide elibhalwe i-Anti Cal McAB kanye ne-colloidal igolide elibhalwe ukuthi unogwaja we-IGG antibody ngaphambi kwesikhathi. Lapho uhlola isampula elimuhle, i-cal kwisampula enamathele negolide elibhalwe ngegolide elibhalwe i-anti lungi, futhi litholakale lihambisane nomucu wokuhlola, i-Cal Conjugate Complex ithwetshiwe yi-anti cal coating MCAB kwi-membrane nefomu "I-Anti Cal Coating yegolide yelebula i-Cal-colloidal legolide anti Ukuqina kombala kuhlobene kahle nokuqukethwe kwekhwala. Isampula elingemuhle alikhiqizi ibhendi yokuhlola ngenxa yokungabikho kweColloidal Gold Comnjugate Cal Coblex. Akunandaba ukuthi i-CAL ikhona ngesampula noma cha, kukhona umugqa obomvu kuvela esifundeni sereferensi kanye nesifunda sokulawula esezingeni eliphezulu, okubhekwa njengekhwalithi yebhizinisi langaphakathi lebhizinisi langaphakathi.

Inqubo yokuhlola:

1. Thatha induku yesampula, ifakwe kwisampula ye-Faeces, bese ubeka induku yesampula emuva, isikulufa uqine futhi unikine kahle, phinda isenzo izikhathi ezi-3. Noma ukusebenzisa induku yesampula kukhethwe amasampula we-50mg Faeces, bese ufaka i-Faeces Samps Tube equkethe i-dilution yesampula, bese isikulufa ngokuqinile.

2. Sebenzisa isampula ye-Pipette elahlekile thatha isampula elincanyana le-Faces kusuka esigulini sohudo, bese ufaka amaconsi ama-3 (cishe u-100Ul) kwishubhu lesampula ye-fecal bese unikina kahle, beka eceleni.

3.ke ukhiphe ikhadi lokuhlola esikhwameni se-foil, ulibeke etafuleni lezinga bese umaka.

I-4.Remove cap kusuka ku-tube yesampula futhi ulahle amasampula amabili okuqala ahlanjululwe, engeza ama-4.Drops (cishe u-100Ul) Ayikho i-Bubble evuselelwe i-verticaly futhi kancane kancane ekhadini elinikeziwe, qala isikhathi.

5.Umphumela kufanele ufundwe kungakapheli imizuzu eyi-10-15, futhi ayilungile ngemuva kwemizuzu eyi-15