Ikhithi yokuxilonga yeI-Thyroid Stimulating Hormoneukuhlolwa kwe-immunochromatographic)
Ukusetshenziswa kwe-in vitro diagnostic kuphela
Sicela ufunde le phakheji ngokucophelela ngaphambi kokuyisebenzisa futhi ulandele ngokuqinile imiyalelo. Ukuthembeka kwemiphumela yokuhlolwa akunakuqinisekiswa uma kukhona ukuchezuka emiyalweni ekule kokufakwa kwephakheji.

UKUSETSHENZISWA OKUHLOSIWE
Ikhithi Yokuxilongwa YeHormone Evuselela Indlala Yegilo (i-fluorescence immunochromatographic assay) iwukuhlolwa kwe-fluorescence immunochromatographic ukuze kutholwe inani le-Thyroid Stimulating Hormone (TSH) ku-serum yomuntu noma ku-plasma, esetshenziswa kakhulu ekuhlolweni komsebenzi we-pituitary-thyroid. Wonke amasampula amahle kufanele aqinisekiswe ngezinye izindlela. Lokhu kuhlolwa kuhloselwe ukusetshenziswa ngochwepheshe bezempilo kuphela.

ISIFINYEZO
Imisebenzi eyinhloko ye-TSH: 1, ikhuthaza ukukhululwa kwamahomoni egilo, i-2, ikhuthaza ukuhlanganiswa kwe-T4, i-T3, kuhlanganise nokuqinisa umsebenzi wepompo ye-iodine, ukuthuthukisa umsebenzi we-peroxidase, ukukhuthaza ukuhlanganiswa kwe-thyroid globulin ne-tyrosine iodide.

ISIMISO SENKQUBO
I-membrane yedivayisi yokuhlola imbozwe nge-anti TSH antibody endaweni yokuhlola kanye ne-anti-rabbit ye-IgG yembuzi endaweni yokulawula. Iphedi elilebulayo limbozwe nge-fluorescence ebhalwe ukuthi anti TSH antibody kanye ne-rabbit IgG kusengaphambili. Lapho kuhlolwa isampula elihle, i-antigen ye-TSH esesampulini ihlangana ne-fluorescence ebhalwe ukuthi i-anti TSH antibody, bese yenza ingxube yamasosha omzimba. Ngaphansi kwesenzo se-immunochromatography, ukugeleza okuyinkimbinkimbi ekuqondeni kwephepha elimuncayo, lapho inkimbinkimbi idlula isifunda sokuhlola, ihlangene ne-anti TSH enamathela antibody, yakha inkimbinkimbi entsha. Izinga le-TSH lihlotshaniswa kahle nesignali ye-fluorescence, kanye nokuhlushwa kwe-TSH. kusampula kungatholwa nge-fluorescence immunoassay assay.

AMA-REAGENTS KANYE NEZINTO EZINIKEZELWAYO

25T izingxenye zephakheji
.Iphepha lokuhlola ngalinye elifakwe esikhwameni nge-desiccant 25T
.Izihlanjululi zesampula
.Faka iphakheji

IZINTO EZIDINGEKAYO KODWA AKUNIKEZWE
Isitsha seqoqo lesampula, isibali sikhathi

UKUQOQWA KANYE NESIGCINISO ESAMPULA
1.Amasampula ahloliwe angaba i-serum, i-heparin anticoagulant plasma noma i-EDTA anticoagulant plasma.
2.Ngokwezinqubo ezijwayelekile qoqa isampula. I-Serum noma isampula ye-plasma ingagcinwa efrijini ku-2-8℃ izinsuku ezingu-7 kanye nokugcinwa kwe-cryopreservation ngaphansi -15°C izinyanga ezingu-6.
3.Wonke amasampula agwema imijikelezo yokuncibilika kweqhwa.

INQUBO YOKUHLOLA
Inqubo yokuhlola yensimbi bheka imanuwali ye-immunoanalyzer. Inqubo yokuhlola i-reagent imi kanje

1.Beka eceleni wonke ama-reagents namasampuli ekamelweni lokushisa.
2.Vula i-Portable Immune Analyzer(WIZ-A101), faka ukungena ngemvume kwephasiwedi ye-akhawunti ngokwendlela yokusebenza yethuluzi, bese ufaka isixhumi esibonakalayo sokubona.
3.Skena ikhodi yokuhlonza ukuze uqinisekise into yokuhlola.
4.Khipha ikhadi lokuhlola esikhwameni se-foil.
5.Faka ikhadi lokuhlola endaweni yekhadi, skena ikhodi ye-QR, bese unquma into yokuhlola.
6.Engeza i-serum engu-20μL noma isampula ye-plasma ukuze uyisampula ye-diluent, bese uxuba kahle.
7.Engeza isisombululo sesampula esingu-80μL ukuze uthole isampula yomthombo wekhadi.
8.Chofoza inkinobho ethi "standard test", ngemva kwemizuzu engu-15, ithuluzi lizobona ngokuzenzakalelayo ikhadi lokuhlola, lingakwazi ukufunda imiphumela esikrinini sokubonisa sensimbi, futhi lirekhode / liphrinte imiphumela yokuhlolwa.
9.Bheka umyalelo we-Portable Immune Analyzer(WIZ-A101).

IMIPHUMELA YOKUHLOLA NOKUTOLIZWA
.Idatha engenhla ingumphumela wokuhlolwa kwe-TSH, futhi kuphakanyiswa ukuthi ilabhorethri ngayinye kufanele isungule uhla lwamanani okuthola i-TSH afanele abantu bakulesi sifunda. Imiphumela engenhla ingeyereferensi kuphela.
.Imiphumela yale ndlela isebenza kuphela kububanzi bereferensi obusungulwe kule ndlela, futhi akukho ukuqhathaniswa okuqondile nezinye izindlela.
.Ezinye izici zingaphinda zibangele amaphutha emiphumeleni yokuthola, okuhlanganisa izizathu zobuchwepheshe, amaphutha okusebenza nezinye izici zesampula.

UKUGCINWA NOKUZInza
1.Ikhithi iyimpilo yeshelufu yezinyanga eziyi-18 kusukela ngosuku lokukhiqizwa kwayo. Gcina amakhithi angasetshenzisiwe ku-2-30°C. UNGAQISHI. Ungasebenzisi ngale kosuku lokuphelelwa yisikhathi.
2.Ungasivuli isikhwama esivaliwe kuze kube yilapho usulungele ukwenza isivivinyo, futhi ukuhlolwa okukodwa kuphakanyiswa ukuthi kusetshenziswe ngaphansi kwendawo edingekayo (izinga lokushisa elingu-2-35℃, umswakama 40-90%) phakathi kwemizuzu engama-60 ngokushesha. ngangokunokwenzeka.
I-3.I-diluent yesampula isetshenziswa ngokushesha ngemva kokuvulwa.

IZIXWAYISO NEZIQINISEKISO
.Ikhithi kufanele ivalwe futhi ivikelwe emswakameni.
.Zonke izibonelo ezinhle zizoqinisekiswa ngezinye izindlela.
.Zonke izifanekiso zizophathwa njengamandla angcolisayo.
.UNGAsebenzisi i-reagent ephelelwe yisikhathi.
.UNGAshintshisani ama-reagents phakathi kwamakhithi anendawo ehlukile No..
.UNGAphinde usebenzise amakhadi okuhlola nanoma yiziphi izisekeli ezilahlwayo.
.Ukungasebenzi kahle, isampula eliningi noma elincane lingaholela ekuphambukeni kwemiphumela.

LUKULINGISA
.Njenganoma yikuphi ukulinganisa okusebenzisa amasosha omzimba egundane, kungenzeka ukuthi kuphazamiseke amasosha omzimba alwa namagundane (HAMA) esibonelweni. Izibonelo zeziguli ezithole amalungiselelo e-monoclonal antibodies ukuze zixilongwe noma zelashwe zingaqukatha i-HAMA. Izibonelo ezinjalo zingadala imiphumela engemihle noma engemihle engamanga.
.Lo mphumela wokuhlolwa ngowenkomba yomtholampilo kuphela, akufanele usebenze njengesisekelo sokuxilongwa nokwelashwa kweziguli, ukuphathwa komtholampilo kweziguli kufanele kucatshangelwe ngokugcwele kuhlanganiswe nezimpawu zayo, umlando wezokwelapha, okunye ukuhlolwa kwaselabhorethri, impendulo yokwelashwa, i-epidemiology kanye nolunye ulwazi. .
.Lesi siphehli sisetshenziswa kuphela ekuhlolweni kwe-serum ne-plasma. Ingase ingatholi umphumela onembile uma isetshenziselwa amanye amasampula afana namathe nomchamo nokunye.

IZIMPAWU ZOKUSEBENZA

IZIKHOMBISI
1.Hansen JH,et al.HAMA Ukuphazamisa Nge-Murine Monoclonal Antibody-Based Immunoassays[J].J ye-Clin Immunoassay,1993,16:294-299.
2.Levinson SS.Imvelo Yama-Heterophilic Antibodies kanye Neqhaza Ekuphazamisekeni Kwe-Immunoassay[J].J ye-Clin Immunoassay,1992,15:108-114.

Ukhiye wezimpawu ezisetshenzisiwe:

	In Vitro Diagnostic Medical Device
	Umkhiqizi
	Gcina ku-2-30 ℃
	Usuku lokuphelelwa isikhathi
	Ungaphinde Usebenzise
	ISEXWAYISO
	Bheka Imiyalo Yokusetshenziswa

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