Le khithi yokuhlola ifanele ukutholwa komthamo we-adrenocorticotropic hormone (ATCH) kusampula ye-Human Plasma ku-Vitro, esetshenziswa kakhulukazi ukuxilonga i-ACTH hypersecretion, i-ACTH ezimele ekhiqiza izicubu ze-pituitary hypopituitarism enokuntuleka kwe-ACTH kanye ne-ectopic ACTH syndrome kufanele kube umphumela wokuhlolwa kwe-ACTH. zihlaziywe kuhlanganiswe nolunye ulwazi lomtholampilo .