Isimiso

I-strip ine-st-ag coating antigen esifundeni sokuhlola kanye nembuzi anti igundane antibody on control esifundeni, esifakwe ku-chromatography kusenesikhathi. I-LAble Pad ihlanganiswe yiGolloidal Gold Beled Mouse-anti Human MCAB kusengaphambili. Lapho uvivinya isampula elinethemba, i-MP-IGM kwisampula ihlangana ne-Colloidal Gold etholakele igundane-anti human Igm McAb, bese ifomu liyinkimbinkimbi. Ngaphansi kwesenzo se-Immunochrotography, okuyinkimbinkimbi futhi isampula ngaphakathi kwe-nitrocellulose membrane flow endaweni etholwa ephepheni, lapho i-antigen yokuhlola, ihlanganiswe ne-antigen ye-MP-AG, i-MP-AN -I-Colloidal Gold enelebuli elibhalwe nge-Mouse-Anti Human MCAB "okuyinkimbinkimbi, ibhendi yokuhlola enemibala yavela esifundeni sokuhlola. Isampula elingemuhle alikhiqizi ibhendi yokuhlola ngenxa yokuvinjelwa kokungavikeki. Akunandaba noma i-MP-IGM ikhona ngesampula noma cha, kukhona umugqa obomvu uvela esifundeni sokulawula okusezingeni eliphezulu, elibhekwa njengezinga lekhwalithi yangaphakathi yebhizinisi.

Inqubo yokuhlola:

Inqubo yokuhlola ye-Wiz-A101 ibona ukufundiswa kwe-Immune Analyzer ephathekayo. Inqubo yokuhlola ebonakalayo imi kanjena:

1. Beka eceleni yonke imiphenduli namasampula wokushisa kwegumbi.
2. Thatha ikhadi lokuhlola kusuka esikhwameni se-foil, ulibeke etafuleni lezinga bese umaka.
3. Faka i-10μL serum noma isampula ye-plasma noma isampula le-20μL eliphelele lesampula kahle lekhadi nge-verttte enikeziwe, bese wengeza i-100μL (cishe nge-2- crop) yesampula.
I-4. Lindela okungenani imizuzu eyi-10-16 bese ufunda umphumela, umphumela awusebenzi ngemuva kwemizuzu engu-15.