ISIMISO

Umucu une-MP-Ag ehlanganisa i-antigen endaweni yokuhlola kanye ne-anti mouse IgG antibody endaweni yokulawula, eboshelwe ku-membrane chromatography kusengaphambili. Iphedi elebula limbozwe ngegolide elicwebezelayo elibhalwe ukuthi i-mouse-anti human IgM McAb kusengaphambili. Lapho kuhlolwa isampula ephozithivu, i-MP-IgM kusampula ihlangana negolide eli-colloidal elibhalwe ukuthi i-mouse-anti human IgM McAb, bese yenza i-immune complex. Ngaphansi kwesenzo se-immunochromatography, inkimbinkimbi nesampula ngaphakathi kolwelwesi lwe-nitrocellulose ligeleza libheke ephepheni elimuncayo, lapho inkimbinkimbi idlula indawo yokuhlola, yahlanganiswa ne-MP-Ag ehlanganisa i-antigen, yakhe “MP-Ag coating antigen-MP. -I-IgM-colloidal gold ebhalwe ukuthi igundane-anti human IgM McAb” inkimbinkimbi, ibhendi yokuhlola enombala yavela endaweni yokuhlola. Isampula elibi alikhiqizi ibhendi yokuhlola ngenxa yokuntuleka kokuzivikela komzimba. Kungakhathaliseki ukuthi i-MP-IgM ikhona kusampula noma cha, kunomugqa obomvu ovela endaweni yokulawula ikhwalithi, ethathwa njengamazinga ebhizinisi angaphakathi ekhwalithi.

Inqubo Yokuhlola:

Inqubo yokuhlola ye-WIZ-A101 bona imiyalelo ye-immune analyzer ephathekayo. Inqubo yokuhlola ebonakalayo imi kanje:

1. Beka eceleni wonke ama-reagents namasampuli ekamelweni lokushisa.
2. Khipha ikhadi lokuhlola esikhwameni se-foil, ulibeke etafuleni lezinga bese ulimaka.
3. Engeza i-serum engu-10μL noma isampula ye-plasma noma isampula legazi eliphelele elingu-20μL ukuze usampule kahle ikhadi nge-dispette enikeziwe, bese wengeza u-100μL (cishe amaconsi angu-2-3) isampula ye-diluent, qala isikhathi.
4. Linda okungenani imizuzu engu-10-15 futhi ufunde umphumela, umphumela awuvumelekile ngemva kwemizuzu engu-15.