ISIMISO

I-membrane yedivayisi yokuhlola ihlanganiswe ne-Group A kanye ne-adenovirus antigen endaweni yokuhlola kanye ne-anti-rabbit ye-IgG yembuzi endaweni yokulawula. Iphedi elilebulayo limbozwe nge-fluorescence ebhalwe ukuthi anti Group A kanye ne-adenovirus kanye ne-rabbit IgG kusengaphambili. Lapho kuhlolwa isampula ephozithivu yeQembu A kanye ne-adenovirus, Iqembu A kanye ne-adenovirus kusampula kuhlangana ne-fluorescence ebhalwe ukuthi i-anti Rotavirus Group A kanye ne-adenovirus, bese yenza ingxube yokuzivikela komzimba. Ngaphansi kwesenzo se-immunochromatography, ukugeleza okuyinkimbinkimbi kubheke ephepheni elimuncayo. Lapho inkimbinkimbi idlula isifunda sokuhlola, yahlanganiswa ne-anti-Rotavirus Group A kanye ne-adenovirus coating antibody, yakha inkimbinkimbi entsha. Uma inegethivu, ayikho i-Rotavirus Group A kanye ne-adenovirus antigen kusampula, ukuze izakhiwo zokuzivikela komzimba zingakwazi ukwakheka, ngeke kube khona umugqa obomvu endaweni yokuthola (T). Kungakhathaliseki ukuthi i-group A rotavirus kanye ne-adenovirus ikhona yini esibonelweni, igundane elibhalwe nge-latex IgG lifakwe ngekhromatograph ukuya endaweni yokulawula ikhwalithi (C) futhi lithathwe yi-anti-mouse IgG antibody. Kuzovela umugqa obomvu endaweni yokulawula ikhwalithi (C). Ulayini obomvu yizinga elivela endaweni yokulawula ikhwalithi (C) ukuze kuhlungwe ukuthi akhona yini amasampula anele nokuthi inqubo ye-chromatography ijwayelekile yini. Iphinde isetshenziswe njengezinga lokulawula langaphakathi lama-reagents.

Inqubo Yokuhlola:

1. Vula ikepisi yeshubhu yeqoqo lesampula. Ungachithi isixazululo ebhodleleni.
2. Khipha udondolo lwesampula, ulufake esampuleni yobulongwe (noma sebenzisa isampula ukuze ukhethe cishe u-50mg wendle), bese ubuyisela induku yesampula, bopha qina bese uyanyakazisa kahle, phinda isenzo izikhathi ezi-3. Thatha ingxenye ehlukile yesampula yendle isikhathi ngasinye. Ngemva kokuthatha isampula, faka intonga yesampula eshubhuni lokuqoqa indle eliqukethe isampula yokuxubha, bese ugoqa i-dropper ngokuqinile. Uma indle yesiguli esihudayo izacile, utshani bepulasitiki obulahlwayo bungasetshenziswa ukwenza amasampula. Ngokusebenzisa amasampula e-pipette alahlwayo thatha isampula yendle elincanyana esigulini sohudo, bese wengeza amaconsi ama-3 (cishe u-100uL) eshubhuni lokusampula le-fecal.
3. Gcoba kahle isampula bese ususa ikepisi ethiphuni bese uyibeka eceleni.
4. Uma igcinwe ekushiseni okuphansi, ikhithi kufanele ibuyiselwe ekamelweni lokushisa ngaphambi kokusetshenziswa. Khipha ikhadi lokuhlola esikhwameni se-foil, ulibeke etafuleni elisezingeni bese ulimaka.
5. Khipha ikepisi kushubhu yesampula bese ulahla amathonsi amabili okuqala esampula ehlanjululwe, engeza amaconsi ama-3 (cishe u-100uL) alikho ibhamuza elihlanjululwe isampula liqonde phezulu futhi kancane ungene emthonjeni wesampula wekhadi nge-dispette enikeziwe, qala isikhathi.
6. Umphumela kufanele ufundwe phakathi kwemizuzu engu-10-15, futhi awuvumelekile ngemva kwemizuzu engu-15.