Isimiso

Ulwelwesi lwedivayisi yokuhlola luhlanganiswe neqembu A ne-Adenovirus Antigen esifundeni sokuhlola kanye nembuzi u-Anti Rabbing IGG antibody on the control. I-Lable Pad ihlanganiswe yi-fluorescence ilebula anti group a ne-adenovirus kanye noRabbit IGG kusengaphambili. Lapho uvivinya isampula elivumayo leqembu A ne-Adenovirus, iqembu A ne-Adenovirus ngesampula lihlangana ne-fluorescence elibhalwe i-anti rotavirus group a ne-adenovirus, futhi akha ingxube yomzimba. Ngaphansi kwesenzo se-Immunochrotography, ukugeleza okuyinkimbinkimbi kokuqondiswa kwephepha lokumunca. Lapho kuyinkimbinkimbi edlula esifundeni sokuhlola, kuhlanganiswe neqembu le-anti-rotavirus a ne-antilody elenhla, amafomu amasha. Uma kungenamibi, alikho iqembu le-rotavirus a ne-adenovirus antigen kusampula, ngakho-ke amasosha omzimba awakwazi ukwakhiwa, ngeke kube nomugqa obomvu endaweni yokutholwa (T). Kungakhathalekile ukuthi iqembu i-rotavirus ne-adenovirus likhona yini ku-specimen, i-IGG ye-latex-elinelebuli elibhalwe nge-chromatography endaweni yokulawula ikhwalithi (c) futhi ithunjwe yimbuzi anti-mouse iGG antilody. Kuzovela umugqa obomvu endaweni yokulawula ikhwalithi (C). Umugqa obomvu yilezi zindlela ezivela endaweni yokulawula ikhwalithi (C) yokwahlulela ukuthi ngabe kukhona amasampula anele nokuthi inqubo ye-chromatomatography ijwayelekile. Iphinde isetshenziswe njengezinga lokulawula langaphakathi lama-reagents.

Inqubo yokuhlola:

1. Vula i-cap ye-tube yengqwela yesampula. Ungachithi ikhambi ebhodleleni.
2 Thatha ingxenye ehlukile yesampula ye-Faeces isikhathi ngasinye. Ngemuva kokusampula, faka induku yesampula kwishubhu lokuqoqa ama-Faeces aqukethe i-dillont eyisampula, bese usula i-dropper kahle. Uma indle yesiguli enesifo sohudo incane, ipulasitiki elahlekile ingasetshenziselwa isampula. Kusetshenziswa isampula ye-Pipette elahlekile thatha isampula le-Finet Fisenes kusuka esigulini sohudo, bese ufaka amaconsi ama-3 (cishe u-100Ul) kwishubhu lesampula le-fecal.
3. Shake kahle isampula bese ususa i-cap ku-dropper tip bese ubeka eceleni.
4. Lapho igcinwe emazingeni okushisa aphansi, ikhithi kufanele ibuyiselwe ekushiseni kwegumbi ngaphambi kokusetshenziswa. Thatha ikhadi lokuhlola esikhwameni se-foil, ulibeke etafuleni lezinga bese umaka.
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I-6. Umphumela kufanele ufundwe kungakapheli imizuzu eyi-10-15, futhi akulungile ngemuva kwemizuzu eyi-15.