I-Pepsinogen I Pepsinogen II kanye nekhithi yokuhlola esheshayo ye-Gastrin-17 Combo
Ikhithi yokuxilonga ye-Pepsinogen I/Pepsinogen II/Gastrin-17
Indlela yokwenza: i-fluorescence immunochromatographic assay
Ulwazi lokukhiqiza
Inombolo Yemodeli | I-G17/PGI/PGII | Ukupakisha | 25 Ukuhlolwa/ikhithi, 30kits/CTN |
Igama | Ikhithi yokuxilonga ye-Pepsinogen I/Pepsinogen II/Gastrin-17 | Ukuhlukaniswa kwezinsimbi | Ikilasi II |
Izici | Ukuzwela okuphezulu, ukusebenza okulula | Isitifiketi | CE/ ISO13485 |
Ukunemba | > 99% | Impilo yeshelufu | Iminyaka Emibili |
Indlela yokwenza | ukuhlolwa kwe-fluorescence immunochromatographic | Isevisi ye-OEM/ODM | Iyatholakala |
UKUSETSHENZISWA OKUHLOSIWE
Le khithi isebenza ekutholweni komthamo we-in vitro wokuhlushwa kwe-Pepsinogen I (PGI), i-Pepsinogen II.
(PGII) kanye ne-Gastrin 17 ku-serum yomuntu/i-plasma/amasampula egazi lonke, ukuhlola iseli ye-gastric oxyntic gland
umsebenzi, isilonda se-gastric fundus mucosa kanye ne-atrophic gastritis. Ikhithi inikeza kuphela umphumela wokuhlola we-Pepsinogen I
(PGI), Pepsinogen II (PGII) kanye ne-Gastrin 17. Umphumela otholiwe uzohlaziywa ngokuhambisana neminye imitholampilo.
ulwazi. Kufanele isetshenziswe kuphela ochwepheshe bezempilo.
Inqubo yokuhlola
1 | Ngaphambi kokusebenzisa i-reagent, funda iphakheji ngokucophelela futhi uzijwayeze nezinqubo zokusebenza. |
2 | Khetha imodi yokuhlola evamile ye-WIZ-A101 ephathekayo yomzimba yokuhlaziya. |
3 | Vula iphakheji yesikhwama se-aluminium foil ye-reagent bese ukhipha idivayisi yokuhlola. |
4 | Faka ngokuvundlile idivayisi yokuhlola ku-slot ye-immune analyzer. |
5 | Ekhasini lasekhaya le-interface yokusebenza ye-immune analyzer, chofoza okuthi "Standard" ukuze ufake isixhumi esibonakalayo sokuhlola |
6 | Chofoza okuthi “QC Scan” ukuze uskene ikhodi ye-QR ohlangothini lwangaphakathi lwekhithi; okokufaka amapharamitha ahlobene nekhithi kuthuluzi kanye khetha uhlobo lwesampula. Qaphela: Inombolo ngayinye yeqoqo lekhithi izoskenwa ngesikhathi esisodwa. Uma inombolo ye-batch iskeniwe, ke yeqa lesi sinyathelo. |
7 | Hlola ukuvumelana "Kwegama Lomkhiqizo", "Inombolo Yeqoqo" njll. Ekuhloleni ukusebenzelana nolwazi olukwikhithi ilebula. |
8 | Ngemuva kokuqinisekiswa kokuvumelana kolwazi, khipha amasampula e-diluent, engeza u-80µL we-serum/plasma/igazi lonke. isampula, futhi uhlanganise ngokwanele. |
9 | Engeza u-80µL wesixazululo esixubile esingenhla kumgodi wesampula wedivayisi yokuhlola. |
10 | Ngemuva kokungeza isampula ephelele, chofoza "Isikhathi" futhi isikhathi sokuhlola esisele sizovezwa ngokuzenzakalelayo ku- esibonakalayo. |
11 | I-immune analyzer izoqedela ukuhlola nokuhlaziya ngokuzenzakalelayo lapho isikhathi sokuhlolwa sesifinyelelwe. |
12 | Isibalo somphumela nesibonisi Ngemuva kokuthi ukuhlolwa kwe-immune analyzer sekuqediwe, umphumela wokuhlolwa uzovezwa esibonakalayo sokuhlola noma ungabukwa ngokusebenzisa “Umlando” ekhasini lasekhaya lesixhumi esibonakalayo sokusebenza. |
Ukusebenza Kwemtholampilo
Ukusebenza kokuhlolwa komtholampilo komkhiqizo kuhlolwa ngokuqoqa amasampula omtholampilo angama-200. Sebenzisa ikhithi ekhangisiwe ye-enzyme exhunywe i-immunosorbent assay njenge-reagent yokulawula. Qhathanisa imiphumela yokuhlolwa kwe-PGI. Sebenzisa ukuhlehla komugqa ukuze uphenye ukuqhathaniswa kwazo. Ama-coefficients okuxhumana okuhlola okubili ngu-y = 0.964X + 10.382 kanye no-R=0.9763 ngokulandelanayo. Qhathanisa imiphumela yokuhlolwa kwe-PGII. Sebenzisa ukuhlehla komugqa ukuze uphenye ukuqhathaniswa kwazo. Ama-coefficients okuxhumana okuhlola okubili ngu-y = 1.002X + 0.025 kanye no-R=0.9848 ngokulandelanayo. Qhathanisa imiphumela yokuhlolwa kwe-G-17. Sebenzisa ukuhlehla komugqa ukuze uphenye ukuqhathaniswa kwazo. Ama-coefficients okuxhumana okuhlola okubili ngu-y =0.983X + 0.079 kanye no-R=0.9864 ngokulandelanayo.
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