ISIMISO

Ulwelwesi lwedivayisi yokuhlola lumbozwe ngamasosha omzimba we-Hp-Ab endaweni yokuhlola kanye ne-anti-rabbit IgG yamasosha omzimba endaweni yokulawula. Iphedi elilebula limbozwe nge-fluorescence ebhalwe ukuthi i-anti Hp-Ag kanye ne-rabbit IgG kusengaphambili. Lapho kuhlolwa isampula elihle, i-Hp-Ab kusampula ihlangana ne-fluorescence enelebuli ye-anti Hp-Ag, bese yenza ingxube yokuzivikela komzimba. Ngaphansi kwesenzo se-immunochromatography, ukugeleza okuyinkimbinkimbi kubheke ephepheni elimuncayo. Lapho inkimbinkimbi idlula isifunda sokuhlola, ihlanganiswe ne-anti-Hp-Ag ehlanganisa i-antibody, yakha inkimbinkimbi entsha. Uma iyinegethivu, awekho ama-antibody e-HP kusampula, ukuze amasosha omzimba angakwazi ukwakheka, ngeke kube khona umugqa obomvu endaweni yokuthola (T). Ulayini obomvu yizinga elivela endaweni yokulawula ikhwalithi (C) ukuze kuhlungwe ukuthi akhona yini amasampula anele nokuthi inqubo ye-chromatography ijwayelekile yini. Iphinde isetshenziswe njengezinga lokulawula langaphakathi lama-reagents.

Inqubo Yokuhlola:

Sicela ufunde okufakiwe kwephakheji ngaphambi kokuhlola.

1. Khipha ikhadi lokuhlola esikhwameni se-foil, ulibeke etafuleni lezinga bese ulimaka.

2. Engeza amaconsi angu-2 esampula ye-serum noma ye-plasma (noma amaconsi angu-3 esampula legazi eliphelele/umunwe) ukuze usampula kahle ikhadi nge-dispette enikeziwe, bese wengeza iconsi elingu-1 lesampula le-diluent, qala isikhathi.

3. Linda okungenani imizuzu eyi-10-15 futhi ufunde umphumela ngemizuzu eyi-10-15. Umphumela awuvumelekile ngemva kwemizuzu engu-15.