Isimiso

Ulwelwesi lwedivayisi yokuhlola luhlanganiswe ne-HP-AB antibody kwisifunda sokuhlola kanye nembuzi anti Unogwaja IGG antibody on the control. I-Lable Pad ihlanganiswe yi-fluorescence ilebula anti HP-AG kanye noRabbit IGG kusengaphambili. Lapho uhlola isampula elimuhle, i-HP-AB yesampula yokuhlangana ne-Fluorescence ilebula anti HP-AG, bese ifomu umxube wokuzivikela. Ngaphansi kwesenzo se-Immunochrotography, ukugeleza okuyinkimbinkimbi kokuqondiswa kwephepha lokumunca. Lapho kuyinkimbinkimbi edlula esifundeni sokuhlola, kuhlanganiswe ne-anti HP-AG Coating antibod, amafomu amasha ayinkimbinkimbi. Uma kungenamibi, akukho antim antibody we-HP kusampula, ukuze kube lula ukuzivikela komzimba, ngeke kube nomugqa obomvu endaweni yokutholwa (T). Umugqa obomvu yilezi zindlela ezivela endaweni yokulawula ikhwalithi (C) yokwahlulela ukuthi ngabe kukhona amasampula anele nokuthi inqubo ye-chromatomatography ijwayelekile. Iphinde isetshenziswe njengezinga lokulawula langaphakathi lama-reagents.

Inqubo yokuhlola:

Sicela ufunde ukufaka iphakethe ngaphambi kokuhlolwa.

1. Thatha ikhadi lokuhlola esikhwameni se-foil, ulibeke etafuleni lezinga bese umaka.

2. Faka amaconsi ama-2 wesampula ye-serum noma ye-plasma (noma amaconsi ama-3 wesampula yegazi eliphelele / i-FialiPIP) ukuze asampule kahle ekhadini elinikeziwe, bese wengeza ithonsi elingu-1 lesampula, qala isikhathi.

3. Lindela okungenani imizuzu engu-10-16 bese ufunda umphumela ngemizuzu engu-10-16. Umphumela awusebenzi ngemuva kwemizuzu engu-15.