Ukubeka kafushane

UVitamin D uyi-Vitamin futhi futhi uyi-hormone ye-steroid, ikakhulukazi kufaka phakathi i-VD2 ne-VD3, intambo yawo ifana kakhulu. UVitamin D3 no-D2 baguqulwa baba ngu-25 hydroxyl Vitamin D (kufaka phakathi i-25-Dihydroxyl Vitamin D3 ne-D2). 25- (oh) vd emzimbeni womuntu, ekwakheni okuzinzile, okuhlushwa okuphezulu. 25- (oh) vd ukhombisa inani eliphelele le-Vitamin D, kanye nekhono lokuguqulwa kwe-Vitamin D, ngakho-ke 25- (oh) vd kubhekwa njengenkomba engcono kakhulu yokuhlola izinga le-Vitamin D.Ikhithi yokuxilonga isuselwe ku I-Immunochrotography futhi inganikeza umphumela kungakapheli imizuzu eyi-15.

Umgomo wenqubo

Ulwelwesi lwedivayisi yokuhlola luhlanganiswe ne-conjugate ye-BSA no-25- (oh) vd esifundeni sokuhlola kanye nembuzi anti unogwaja IGG antibody on the control. I-Marker Pad ihlanganiswe ngu-Fluorescence Mark anti 25- (oh) VD antibody kanye noRabbit IGG kusengaphambili. Lapho uhlola isampula, 25- (oh) vd ngesampula ehlangana ne-fluorescence emakwe anti 25- (oh) vd antibody, bese wenza ingxube yomzimba. Ngaphansi kwesenzo se-Immunochrotography, ukugeleza okuyinkimbinkimbi kokuqondiswa kwephepha lokumunca, lapho i-Complex idlulisela isifunda sokuhlola, umaki wamahhala we-fluorescent uzohlanganiswa ne-25- (oh) VD ku-membrane.I-ah) I-VD ukuxhumeka okungalungile kwesiginali ye-fluorescence, kanye nokuhlushwa okungu-25- (oh) vd ngesampula yesampula kungatholwa yi-fluorencem immunoassay assay.