ISIFINYEZO

I-Vitamin D iyivithamini futhi iyi-hormone ye-steroid, ikakhulukazi ehlanganisa i-VD2 ne-VD3, imiyalelo yayo efana kakhulu. I-Vitamin D3 kanye ne-D2 iguqulwa ibe yi-25 hydroxyl vitamin D (kuhlanganise ne-25-dihydroxyl vitamin D3 kanye ne-D2). I-25-(OH) VD emzimbeni womuntu, ukufundisa okuzinzile, ukugxila okuphezulu. I-25-(OH) VD ibonisa inani eliphelele likavithamini D , kanye nekhono lokuguqulwa likavithamini D, ngakho-ke i-25-(OH)VD ibhekwa njengenkomba engcono kakhulu yokuhlola izinga likavithamini D. Ikhithi Yokuxilongwa isekelwe i-immunochromatography futhi inganikeza umphumela kungakapheli imizuzu eyi-15.

ISIMISO SENKQUBO

I-membrane yedivayisi yokuhlola ihlanganiswe ne-conjugate ye-BSA kanye ne-25-(OH)VD endaweni yokuhlola kanye ne-anti-rabbit IgG ye-antibody yembuzi endaweni yokulawula. Iphedi lomaka limbozwe nge-fluorescence mark anti-25-(OH)VD antibody kanye ne-IgG yonogwaja kusengaphambili. Lapho kuhlolwa isampula, i-25-(OH)VD kusampula ihlangana ne-fluorescence ephawulwe ukuthi i-anti 25-(OH)VD antibody, bese yenza ingxube yamasosha omzimba. Ngaphansi kwesenzo se-immunochromatography, ukugeleza okuyinkimbinkimbi kubheke ephepheni elimuncayo, lapho okuyinkimbinkimbi kudlula isifunda sokuhlola, Umaka we-fluorescent wamahhala uzohlanganiswa no-25-(OH)VD kulwelwesi.Ukuhlushwa okungu-25-(OH) I-VD iwukuhlobana okunegethivu kwesignali ye-fluorescence, futhi ukugxila kwe-25-(OH)VD kusampula kungatholwa nge-fluorescence immunoassay assay.