ISIFINYEZO

I-Vitamin D iyi-vitamin futhi iyi-hormone ye-steroid, ikakhulukazi ehlanganisa i-VD2 ne-VD3, ukwakheka kwayo kufana kakhulu. I-Vitamin D3 ne-D2 ziguqulwa zibe yi-25 hydroxyl vitamin D (kufaka phakathi i-25-dihydroxyl vitamin D3 ne-D2). I-25-(OH) VD emzimbeni womuntu, ukwakheka okuzinzile, ukuhlushwa okuphezulu. I-25-(OH) VD ibonisa inani eliphelele le-vitamin D, kanye nekhono lokuguqula i-vitamin D, ngakho-ke i-25-(OH)VD ibhekwa njengesibonakaliso esihle kakhulu sokuhlola izinga le-vitamin D. I-Diagnostic Kit isekelwe ku-immunochromatography futhi inganikeza umphumela zingakapheli imizuzu eyi-15.

ISIMISO SENQUBO

I-membrane yedivayisi yokuhlola imbozwe nge-conjugate ye-BSA kanye ne-25-(OH)VD esifundeni sokuhlola kanye ne-antibody yembuzi yokulwa nonogwaja IgG esifundeni sokulawula. Iphedi yomaka imbozwe nge-antibody ye-fluorescence mark anti 25-(OH)VD kanye ne-IgG yonogwaja kusengaphambili. Lapho kuhlolwa isampula, i-25-(OH)VD kusampula ihlangana ne-antibody ye-antibody ye-fluorescence mark anti 25-(OH)VD, bese yakha ingxube yokuzivikela komzimba. Ngaphansi kwesenzo se-immunochromatography, ukugeleza okuyinkimbinkimbi kuya ohlangothini lwephepha elimuncayo, lapho i-complex idlula esifundeni sokuhlola, i-free fluorescent marker izohlanganiswa ne-25-(OH)VD ku-membrane. Ukuhlushwa kwe-25-(OH)VD kuwukuxhumana okungekuhle kwesignali ye-fluorescence, futhi ukuhlushwa kwe-25-(OH)VD kusampula kungatholakala nge-fluorescence immunoassay assay.