Isingeniso: Ukubaluleka Kwezokwelapha Kokuqapha Umsebenzi Wezinso Kwasekuqaleni:

Isifo sezinso esingamahlalakhona (CKD) sesibe yinselele yezempilo yomphakathi emhlabeni jikelele. Ngokwezibalo ezivela kwi-World Health Organisation, cishe abantu abayizigidi ezingu-850 emhlabeni wonke bahlushwa izifo zezinso ezahlukahlukene, kanti ukusabalala kwesifo sezinso esingamahlalakhona emhlabeni wonke cishe kungama-9.1%. Okubi kakhulu ukuthi isifo sezinso esingamahlalakhona sokuqala ngokuvamile asinazo izimpawu ezicacile, okwenza inani elikhulu leziguli liphuthelwe isikhathi esingcono kakhulu sokungenelela. Ngokuphikisana nalesi simo,i-microalbuminuria, njengesibonakaliso esibucayi sokulimala kwezinso kwasekuqaleni, sekuye kwaba usizo kakhulu. Izindlela zokuhlola ukusebenza kwezinso zendabuko njenge-serum creatinine kanye nesilinganiso sokuhlunga se-glomerular esilinganiselwe (i-eGFR) zizobonisa ukukhubazeka kuphela lapho ukusebenza kwezinso kulahleka ngaphezu kwama-50%, kuyilapho ukuhlolwa kwe-albumin yomchamo kunganikeza izimpawu zesixwayiso zakuqala lapho ukusebenza kwezinso kulahleka ngo-10-15%.

Inani lezokwelapha kanye nesimo samanje seI-ALBukuhlolwa komchamo

I-Albumin (i-ALB) yiprotheni eningi kakhulu emchameni wabantu abaphilile, enesilinganiso esijwayelekile sokukhishwa kwe-albhamuin esingaphansi kwamahora angama-30mg/24. Uma izinga lokukhishwa kwe-albhamuin emchameni liphakathi kwamahora angama-30-300mg/24, lichazwa njenge-microalbuminuria, futhi lesi sigaba yisikhathi se-golden window sokungenelela ukuze kulungiswe umonakalo wezinso. Njengamanje, lesi sigaba sivame ukusetshenziswa.I-ALBIzindlela zokuthola emtholampilo zifaka phakathi i-radioimmunoassay, i-enzyme-linked immunosorbent assay (ELISA), i-immunoturbidimetry, njll., kodwa lezi zindlela ngokuvamile zinezinkinga ezifana nokusebenza okuyinkimbinkimbi, ukusetshenziswa isikhathi eside, noma isidingo semishini ekhethekile. Ikakhulukazi ezikhungweni zezokwelapha eziyinhloko kanye nezimo zokuqapha ekhaya, ubuchwepheshe obukhona kunzima ukuhlangabezana nezidingo zobulula, isivinini, kanye nokunemba, okuholela ekutheni inani elikhulu leziguli ezinomonakalo wezinso kusenesikhathi zingatholakali ngesikhathi.

Ukuthuthukiswa Okusha KokunembaUkuhlolwa Komchamo we-ALBI-Reagent

Ngenxa yokulinganiselwa kobuchwepheshe bokuhlola obukhona, inkampani yethu ithuthukise i-PrecisionUkuhlolwa Komchamo we-ALB I-Reagent ukuze kufezwe izimpumelelo eziningi zobuchwepheshe. I-reagent isebenzisa ubuchwepheshe obuthuthukisiwe be-immunochromatographic obune-affinity ephezulu kanye ne-antibody ye-albumin monoclonal ecacile kakhulu ukuqinisekisa ukunemba nokuthembeka kokuhlolwa. Ubuchwepheshe obusha bubonakala kakhulu ezicini ezintathu:

• Ukuzwela okuthuthukisiwe kakhulu: umkhawulo ophansi wokutholwa ufinyelela ku-2mg/L, futhi uyakwazi ukubona ngokunembile umkhawulo womchamo we-microalbumin ongu-30mg/24h, okungcono kakhulu kunokuzwela kwemigqa yokuhlola yendabuko.
• Ikhono elithuthukisiwe lokulwa nokuphazamiseka: Ngokuklanywa kwesistimu ye-buffer eyingqayizivele, inganqoba ngempumelelo ukuphazamiseka kokushintshashintsha kwe-pH yomchamo, izinguquko zamandla e-ionic kanye nezinye izici emiphumeleni yokuhlolwa, okuqinisekisa ukuzinza kokuhlolwa ngaphansi kwezimo ezahlukene zomzimba.
• Ukutholwa kobuningi obusha: umfundi okhethekile osekelayo angabona ukutholwa kobuningi obuphakathi kuya kobuningi, ububanzi bokutholwa buhlanganisa u-0-200mg/L, ukuze kuhlangatshezwane nezidingo ezahlukene zezokwelapha kusukela ekuhlolweni kuya ekuqapheni.

Ukusebenza Komkhiqizo kanye Nezinzuzo

Njengoba iqinisekiswe ngokwezokwelapha ezibhedlela eziningana zezinga eliphezulu, le reagent ikhombisa izinkomba zokusebenza ezinhle kakhulu. Uma kuqhathaniswa nesilinganiso segolide se-albhamuin yomchamo samahora angama-24, i-coefficient yokuxhumana ifinyelela ngaphezu kuka-0.98; ama-coefficients angaphakathi naphakathi kwe-batch of variation angaphansi kuka-5%, aphansi kakhulu kunezinga lomkhakha; isikhathi sokuthola singamaminithi angu-15 kuphela, okuthuthukisa kakhulu ukusebenza kahle komsebenzi wezokwelapha. Izinzuzo zomkhiqizo zifingqiwe ngezansi:

• Ukulula kokusebenza: asikho isidingo sokwelashwa okuyinkimbinkimbi kwangaphambi kokwelashwa, amasampula omchamo angaba ngqo kusampula, ukuhlinzwa kwezinyathelo ezintathu ukuqedela ukuhlolwa, abangebona ochwepheshe bangakwazi ukukufunda kahle ngemva kokuqeqeshwa okufushane.
• Imiphumela enembile: ukusetshenziswa kohlelo lokuthuthukisa umbala olucacile, iso elingabonakali lingafundwa ekuqaleni, amakhadi ombala afanayo angaba ukuhlaziywa kwesilinganiso esincane, ukuze kuhlangatshezwane nezidingo zezimo ezahlukene zohlelo lokusebenza.
• Kuyonga futhi kusebenza kahle: izindleko zokuhlolwa okukodwa ziphansi kakhulu kunezokuhlolwa kwelebhu, ezifanele ukuhlolwa okukhulu kanye nokuqapha isikhathi eside, futhi zinenani elivelele kwezomnotho kwezempilo.

• Inani lesixwayiso kusenesikhathi: umonakalo wezinso ungatholakala eminyakeni engu-3-5 ngaphambi kwezinkomba zokusebenza kwezinso zendabuko, okuthola isikhathi esibalulekile sokungenelela kwezokwelapha.

Izimo zokusetshenziswa kwemitholampilo kanye nezincomo zesiqondiso

UkunembaUkuhlolwa komchamo we-ALBtinezimo eziningi zokusetshenziswa. Emkhakheni wesifo sikashukela, iziqondiso ze-American Diabetes Association (ADA) zincoma ngokusobala ukuthi zonke iziguli ezinesifo sikashukela sohlobo 1 ≥ iminyaka emi-5 kanye nazo zonke iziguli ezinesifo sikashukela sohlobo 2 kufanele zihlolwe i-albumin yomchamo minyaka yonke. Ekuphathweni komfutho wegazi ophakeme, iziqondiso ze-ESC/ESH zomfutho wegazi ophakeme zibala i-microalbuminuria njengophawu olubalulekile lomonakalo wezitho zomzimba eziqondiwe. Ngaphezu kwalokho, i-reagent ifaneleka ezimweni eziningi ezifana nokuhlolwa kwengozi yesifo senhliziyo, ukuhlolwa kokusebenza kwezinso kubantu asebekhulile, kanye nokuqapha izinso ngesikhathi sokukhulelwa.

Okuthakazelisa kakhulu ukuthi lo mkhiqizo ufanelana kahle nezidingo zokuxilongwa nokwelashwa ngokwezigaba. Ungasetshenziswa njengethuluzi lokuhlola eliphumelelayo lesifo sezinso ezikhungweni zezokwelapha eziyinhloko njengezibhedlela zomphakathi kanye nezikhungo zezempilo zasedolobheni; eminyangweni ye-nephrology kanye ne-endocrinology yezibhedlela ezijwayelekile, ungasetshenziswa njengethuluzi elibalulekile lokuphathwa kwezifo kanye nokuqapha ukusebenza kahle; ezikhungweni zokuhlolwa kwezokwelapha, ungafakwa emaphaketheni okuhlolwa kwezempilo ukuze kwandiswe izinga lokutholakala kokulimala kwezinso kusenesikhathi; futhi kulindeleke ukuthi ungene emakethe yokuqapha impilo yomndeni ngemuva kokuqinisekiswa okwengeziwe esikhathini esizayo.

Isiphetho

I-Baysen Medical ihlala igxile ebuchwephesheni bokuxilonga ukuze ithuthukise ikhwalithi yempilo. Sithuthukise amapulatifomu obuchwepheshe amahlanu - i-Latex, igolide le-colloidal, i-Fluorescence Immunochromatographic Assay, i-Molecular, i-Chemiluminescence Immunoassay.Ukuhlolwa kwe-ALB FIA Ukuqapha ukulimala kwezinso esigabeni sokuqala