UKUSETSHENZISWA OKUHLOSIWE

Ikhithi yokuxilonga yeCalprotectin(cal) iyi-colloidal gold immunochromatographic assay yokunqunywa kwe-semiquantitative ye-cal evela endle yomuntu, enokuxilongwa okubalulekile kwensiza.
ukubaluleka kwesifo samathumbu esivuvukalayo. Lokhu kuhlola kuyi-reagent yokuhlola. Wonke amasampula amahle kufanele abe
kuqinisekiswe ezinye izindlela. Lokhu kuhlolwa kuhloselwe ukusetshenziswa ngochwepheshe bezempilo kuphela.
Okwamanje, lokhu kuhlolwa kusetshenziselwa i-IVD, izinsimbi ezengeziwe azidingeki.
ISIFINYEZO
Cal iyi-heterodimer, eyakhiwe nge-MRP 8 kanye ne-MRP 14. Itholakala kuma-neutrophils cytoplasm
futhi kuvezwe kulwelwesi lwamaseli e-mononuclear. I-Cal iyi-acute phase proteins, inezinzile kahle
esigabeni esingaba yisonto elilodwa endle yomuntu, kunqunywa ukuthi kube wuphawu lwesifo samathumbu esivuvukalayo.
Ikhithi iwukuhlola okulula, okubonakalayo kwe-semiqualitative okuthola i-cal endle yomuntu, ikwazi ukutholwa okuphezulu.
ukuzwela nokucacisa okuqinile. Ukuhlola okusekelwe kusemishi ephezulu yamasosha omzimba amabili
umgomo wokusabela kanye nobuchwepheshe bokuhlaziya i-immunochromatographic assay, kunganikeza umphumela
phakathi nemizuzu engu-15.
ISIMISO SENKQUBO
Umucu une-anti cal coating McAb endaweni yokuhlola kanye ne-anti-rabbit IgG antibody elawulayo.
isifunda, esiboshelwe ku-membrane chromatography kusengaphambili. I-lable pad imbozwe nge
igolide eliyi-colloidal elibhalwe ukuthi i-anti cal McAb kanye ne-colloidal yegolide ebhalwe i-rabbit IgG antibody kusengaphambili.
Lapho kuhlolwa isampula evumayo, i-cal kusampula ihlanganiswe negolide eline-colloidal elibhalwe ukuthi i-anti cal McAb,
futhi yakhe i-immune complex, njengoba ivunyelwe ukufuduka eduze komugqa wokuhlola, i-cal conjugate
i-complex ithathwa yi-anti cal coating McAb kulwelwesi bese yenza “i-anti cal coating
I-McAb-cal-colloidal gold ebhalwe ukuthi anti cal McAb” complex, ibhendi yokuhlola enombala yavela lapho ihlolwa.
isifunda. Ukuqina kombala kuhlotshaniswa kahle nokuqukethwe kwekhalori. Isampula elibi alikwenzi
khiqiza ibhendi yokuhlola ngenxa yokungabikho kwe-colloidal gold conjugate cal complex. Akunandaba ukuthi cal
ekhona kusampula noma cha, kukhona umugqa obomvu ovela endaweni yereferensi nokulawulwa kwekhwalithi
isifunda, esithathwa njengamazinga ebhizinisi angaphakathi ayikhwalithi.

Ukuhlolwa kwethu kwe-CAL yimboni yokuqala yokuthola i-CFDA e-China.Sesivele siyithumela emazweni amaningi ngayo yonke impendulo eqondile.

Siyakwamukela ukuxhumana nathi ukuze uthole imininingwane eyengeziwe.