UKUSETSHENZISWA OKUHLOSIWE

Ikhithi Yokuxilonga yeCalprotectin (cal) iwukuhlolwa kwe-colloidal gold immunochromatographic kokunqunywa kwe-semiquantitative ye-cal evela endle yabantu, okune-accessory ebalulekile yokuxilonga
inani lesifo sokuvuvukala kwamathumbu. Lokhu kuhlolwa kuyi-reagent yokuhlola. Zonke izibonelo ezibonisa ukuthi kukhona umdlavuza kumele zibe
kuqinisekiswe ezinye izindlela. Lokhu kuhlolwa kuhloselwe ukusetshenziswa kochwepheshe bezempilo kuphela.
Okwamanje, lolu vivinyo lusetshenziselwa i-IVD, akudingeki amathuluzi engeziwe.
ISIFINYEZO
I-Cal iyi-heterodimer, eyakhiwa yi-MRP 8 kanye ne-MRP 14. Itholakala ku-neutrophils cytoplasm
futhi ivezwa kuma-membrane eseli elilodwa lenuclear. I-Cal iyi-acute phase proteins, iqinile kahle
Uma kwenzeka cishe isonto elilodwa endle yabantu, kutholakala ukuthi kuyisifo sokuvuvukala kwamathumbu.
Le khithi iyindlela elula yokuhlola ebonakalayo, elinganiselayo ethola ama-cal endle yabantu, futhi ithola kakhulu
ukuzwela kanye nokucaciswa okunamandla. Ukuhlolwa okusekelwe kusandwich yama-antibodies amabili akhethekile
isimiso sokusabela kanye nobuchwepheshe bokuhlaziya i-immunochromatographic assay, kunganikeza umphumela
kungakapheli imizuzu eyi-15.
ISIMISO SENQUBO
I-strip ine-anti-cal coating McAb endaweni yokuhlola kanye ne-antibody ye-IgG yokulwa nonogwaja wembuzi elawulwayo.
isifunda, esiboshelwe ku-membrane chromatography kusengaphambili. Iphedi yelebula imbozwe yi-
i-colloidal gold enelebula le-anti cal McAb kanye ne-colloidal gold enelebula le-rabbit IgG antibody kusengaphambili.
Lapho kuhlolwa isampula enhle, i-cal esampula yayinegolide le-colloidal elibhalwe ukuthi i-anti cal McAb,
futhi yakha i-immune complex, njengoba ivunyelwe ukuhamba eceleni komcu wokuhlola, i-cal conjugate
I-complex ibanjwa yi-anti-cal coating McAb kulwelwesi bese yakha “i-anti-cal coating
I-McAb-cal-colloidal gold ebhalwe ukuthi anti cal McAb” complex, ibhendi yokuhlola enemibala yavela esivivinyweni
isifunda. Ubukhulu bombala buhlobene kahle nokuqukethwe kwama-cal. Isampula engemihle ayisho
khiqiza ibhendi yokuhlola ngenxa yokungabikho kwe-colloidal gold conjugate cal complex. Kungakhathaliseki ukuthi i-cal iyini
uma ikhona kusampula noma cha, kuvela umugqa obomvu endaweni yokubhekisela kanye nokulawulwa kwekhwalithi
isifunda, esibhekwa njengezindinganiso zekhwalithi zangaphakathi zebhizinisi.

Ukuhlolwa kwethu kwe-CAL kuyimboni yokuqala ukuthola i-CFDA eShayina. Sesivele sithumela emazweni amaningi ngempendulo enhle.

Siyakwamukela ukuxhumana nathi ukuthola imininingwane eyengeziwe.