Ikhithi yokuxilonga ye-Myoglobin (i-fluorescence immunochromatographic assay)

Ukusetshenziswa kwe-in vitro diagnostic kuphela

Sicela ufunde le phakheji ngokucophelela ngaphambi kokuyisebenzisa futhi ulandele ngokuqinile imiyalelo. Ukuthembeka kwemiphumela yokuhlolwa akunakuqinisekiswa uma kukhona ukuchezuka emiyalweni ekule kokufakwa kwephakheji.

UKUSETSHENZISWA OKUHLOSIWE

Ikhithi yokuxilonga ye-myoglobin (i-fluorescence immunochromatographic assay) iwukuhlolwa kwe-fluorescence immunochromatographic ukuze kutholwe inani le-myoglobin (MYO) ku-serum yomuntu noma i-plasma, esetshenziswa kakhulu njengosizo ekuxilongeni kwe-acute myocardial infarction. Lokhu kuhlola kuhloselwe ukusetshenziswa kochwepheshe bezokunakekelwa kwempilo kanye nokusetshenziswa kochwepheshe basekhaya kuphela.

ISIMISO SENKQUBO

I-membrane yedivayisi yokuhlola ihlanganiswe ne-anti-MYO antibody endaweni yokuhlola kanye ne-anti-rabbit ye-IgG yembuzi endaweni yokulawula. Iphedi elilebula limbozwa nge-fluorescence ebhalwe ukuthi i-anti MYO antibody kanye ne-rabbit IgG kusengaphambili. Lapho kuhlolwa isampula, i-antigen ye-MYO kusampula ihlangana ne-fluorescence ebhalwe ukuthi i-anti MYO, bese yenza ingxube yokuzivikela komzimba. Ngaphansi kwesenzo se-immunochromatography, ukugeleza okuyinkimbinkimbi kubheke ephepheni elimuncayo. Lapho inkimbinkimbi idlula isifunda sokuhlola, ihlanganiswe ne-anti-MYO yokuhlanganisa i-antibody, yakha inkimbinkimbi entsha. Izinga le-MYO lihlotshaniswa kahle nesignali ye-fluorescence, futhi ukugxila kwe-MYO kusampula kungatholwa nge-fluorescence immunoassay assay.