Ikhithi yokuxilonga ye-Myoglobin (i-fluorescence immunochromatographic assay)

Ukusetshenziswa kwe-in vitro diagnostic kuphela

Sicela ufunde lokhu kufakwa kwephakheji ngokucophelela ngaphambi kokuyisebenzisa futhi ulandele ngokuqinile imiyalelo. Ukuthembeka kwemiphumela yokuhlolwa akunakuqinisekiswa uma kukhona ukuchezuka emiyalweni ekule kokufakwa kwephakheji.

UKUSETSHENZISWA OKUHLOSIWE

Ikhithi yokuxilonga ye-myoglobin (i-fluorescence immunochromatographic assay) iwukuhlolwa kwe-fluorescence immunochromatographic ukuze kutholwe inani le-myoglobin (MYO) ku-serum yomuntu noma i-plasma, esetshenziswa kakhulu njengosizo ekuxilongeni kwe-acute myocardial infarction. Lokhu kuhlola kuhloselwe ukusetshenziswa kochwepheshe bezokunakekelwa kwempilo kanye nokusetshenziswa kochwepheshe basekhaya kuphela.

ISIMISO SENKQUBO

I-membrane yedivayisi yokuhlola ihlanganiswe ne-anti-MYO antibody endaweni yokuhlola kanye ne-anti-rabbit ye-IgG yembuzi endaweni yokulawula. Iphedi elilebula limbozwa nge-fluorescence ebhalwe ukuthi i-anti MYO antibody kanye ne-rabbit IgG kusengaphambili. Lapho kuhlolwa isampula, i-antigen ye-MYO kusampula ihlangana ne-fluorescence ebhalwe ukuthi i-anti MYO, bese yenza ingxube yokuzivikela komzimba. Ngaphansi kwesenzo se-immunochromatography, ukugeleza okuyinkimbinkimbi kubheke ephepheni elimuncayo. Lapho inkimbinkimbi idlula isifunda sokuhlola, ihlanganiswe ne-anti-MYO yokuhlanganisa i-antibody, yakha inkimbinkimbi entsha. Izinga le-MYO lihlotshaniswa kahle nesignali ye-fluorescence, futhi ukugxila kwe-MYO kusampula kungatholwa nge-fluorescence immunoassay assay.