ISIMISO

Ulwelwesi lwedivayisi yokuhlola lumbozwe nge-ALB antigen endaweni yokuhlola kanye ne-anti-rabbit IgG yamasosha omzimba endaweni yokulawula. Iphedi lomaka limbozwe ngophawu lwe-fluorescence anti anti ALB kanye ne-rabbit IgG kusengaphambili. Lapho kuhlolwa isampula, i-ALB kusampula ihlangana ne-fluorescence ephawulwe ukuthi i-anti-ALB antibody, bese yenza ingxube yokuzivikela komzimba. Ngaphansi kwesenzo se-immunochromatography, ukugeleza okuyinkimbinkimbi ekuqondeni kwephepha elimuncayo, lapho inkimbinkimbi idlula isifunda sokuhlola, Umaka we-fluorescent wamahhala uzohlanganiswa ne-ALB kulwelwesi.Ukuhlushwa kwe-ALB kuwukuhlobana okungalungile kwesignali ye-fluorescence, futhi ukugxila kwe-ALB kusampula kungatholwa nge-fluorescence immunoassay assay.

Inqubo Yokuhlola:

Sicela ufunde imanuwali yokusebenza kwezinsimbi kanye nokufakwa kwephakheji ngaphambi kokuhlola.

1. Beka eceleni wonke ama-reagents namasampuli ekamelweni lokushisa.
2. Vula i-Portable Immune Analyzer(WIZ-A101), faka ukungena ngemvume kwephasiwedi ye-akhawunti ngokwendlela yokusebenza yethuluzi, bese ufaka isixhumi esibonakalayo sokubona.
3. Skena ikhodi yokuhlonza ukuze uqinisekise into yokuhlola.
4. Khipha ikhadi lokuhlola esikhwameni se-foil.
5. Faka ikhadi lokuhlola endaweni yekhadi, skena ikhodi ye-QR, bese unquma into yokuhlola.
6. Engeza isampula yomchamo ongu-80μL ukuze uthole umthombo wekhadi
7. Chofoza inkinobho ethi "ukuhlolwa okujwayelekile", ngemuva kwemizuzu engu-15, ithuluzi lizobona ngokuzenzakalelayo ikhadi lokuhlola, lingakwazi ukufunda imiphumela esikrinini sokubonisa sensimbi, futhi lirekhode/liphrinte imiphumela yokuhlolwa.
8. Bheka imiyalelo ye-Portable Immune Analyzer(WIZ-A101).