ISIMISO

I-membrane yedivayisi yokuhlola imbozwe nge-anti EV71 antibody endaweni yokuhlola kanye ne-anti-rabbit IgG antibody yembuzi endaweni yokulawula. Iphedi elebulayo limbozwe nge-fluorescence ebhalwe ukuthi i-anti EV71 antibody kanye ne-rabbit IgG kusengaphambili. Lapho kuhlolwa isampula elihle, i-antigen ye-EV71 kusampula ihlangana ne-fluorescence ebhalwe ukuthi i-anti EV71 antibody, bese yenza ingxube yamasosha omzimba. Ngaphansi kwesenzo se-chromatography, ukugeleza okuyinkimbinkimbi kubheke ephepheni elimuncayo, lapho inkimbinkimbi idlula isifunda sokuhlola, ihlanganiswe ne-anti EV71 yokuhlanganisa i-antibody, yakha inkimbinkimbi entsha.

Uma inegethivu, isampula aliqukethe i-enterovirus 71 IgM antibody, ukuze i-immune complex ingakwazi ukwakheka. Ngeke kube khona umugqa obomvu endaweni yokuthola (T). Kungakhathaliseki ukuthi i-Enterovirus 71 IgM antibody ikhona esibonelweni noma cha, igundane elisele elibhalwe ngegolide eline-colloidal anti-human IgM monoclonal antibody kanye ne-anti-mouse IgG antibody ehlanganiswe endaweni yokulawula ikhwalithi (C) iyabopha. Khona-ke ama-agglutinates athuthukisa umbala endaweni yokulawula ikhwalithi, futhi umugqa obomvu uzovela ku-(C). Ulayini obomvu yizinga elivela endaweni yokulawula ikhwalithi (C) ukuze kuhlungwe ukuthi akhona yini amasampula anele nokuthi inqubo ye-chromatography ijwayelekile yini. Iphinde isetshenziswe njengezinga lokulawula langaphakathi lama-reagents.

Inqubo Yokuhlola:

1.Amasampula ahloliwe angaba yigazi eliphelele, okuhlanganisa igazi le-venous noma igazi le-Peripheral. Igazi eliphelele alikwazi ukugcinwa ngemva kokuqoqwa. Kufanele ngisetshenziswe ngokushesha ngemva kokuqoqa.

Amasampula e-2.Serum aqoqwa ngokwe-aseptically ngokuya ngamasu ajwayelekile. I-serum engasebenzi kahle ayikwazi ukusetshenziswa. Akunconywa ukusebenzisa i-lipemic, i-turbid noma i-serum engcolile. Ingxenye ye-particle ku-serum. Futhi imvula izothinta imiphumela yokuhlolwa, amasampuli anjalo kufanele afakwe phakathi noma ahlungwe ngaphambi kokusetshenziswa.

3.Amasampula ahlolwe angaba i-heparin, i-Sodium citrate noma i-EDTA anticoagulant plasma.

4.Ngokwezinqubo ezijwayelekile qoqa isampula. I-Serum noma isampula ye-plasma ingagcinwa efrijini ku-2-8℃ izinsuku ezingu-3 kanye nokugcinwa kwe-cryopreservation ngaphansi -15°C izinyanga ezingu-3.

5.Wonke amasampula agwema imijikelezo yokuncibilika kweqhwa.