Isimiso

Ulwelwesi lwedivayisi yokuhlola luhlanganiswe ne-antibod antibody esifundeni sokuhlola kanye nembuzi anti Unogwaja IGG antibody on the control. I-LAble Pad ihlanganiswe yi-fluorescence ilebula anti ev71 antibody kanye noRabbit Igg kusengaphambili. Lapho uhlola isampula elinethemba, i-antigen e-EV71 yesampula ihlangana ne-fluorescence ilebuli anti ev71 antibody, bese wenza umxube wokuzivikela. Ngaphansi kwesenzo se-Chromatography, ukugeleza okuyinkimbinkimbi kokuqondisa kwephepha lokumunca, lapho kuyinkimbinkimbi kudlula esifundeni sokuhlola, kuhlanganiswe ne-antibod antibody, amafomu amasha.

Uma kungenamibi, isampula ayinayo i-Enterovirus 71 Igm antimm antiilody, ukuze inhlanga iyinkimbinkimbi ikwazi ukwakhiwa. Ngeke kube khona umugqa obomvu endaweni yokutholwa (T). Akunandaba noma ngabe i-Entevirus 71 i-IGM anti antibody ikhona ku-specimen noma cha, i-colloidal esele yegolide enelembu lokulwa ne-iGM monoclonal antibody ne-IGG antibody ehlanganiswe endaweni yokulawula ikhwalithi (C) izibopha. Lapho-ke ama-agglutitates athuthukisa umbala endaweni yokulawula ikhwalithi, futhi ulayini obomvu uzovela (c). Umugqa obomvu yilezi zindlela ezivela endaweni yokulawula ikhwalithi (C) yokwahlulela ukuthi ngabe kukhona amasampula anele nokuthi inqubo ye-chromatomatography ijwayelekile. Iphinde isetshenziswe njengezinga lokulawula langaphakathi lama-reagents.

Inqubo yokuhlola:

1.Izibonelo ezivivinywe zingaba yigazi elizonke, kufaka phakathi igazi le-venous noma igazi elihlanzekile. Igazi lonke alikwazi ukugcinwa ngemuva kokuqoqa. Kufanele ngisetshenziswe kungekudala ngemuva kokuqoqa.

Amasampula we-2.Serum aqoqwa ngokuya ngamasu ajwayelekile. I-serum eshisiwe engasebenzi. Akunconyelwe ukusebenzisa i-Liphemid, eTurbid noma i-Serum engcolisiwe. Udaba lokukhipha i-serum. Futhi ukuhanjiswa kwezulu kuzothinta imiphumela yokuhlola, amasampula anjalo kufanele afakwe ku-centrifug noma ahlungiwe ngaphambi kokusetshenziswa.

3.Izinsuku ezihlolwe zingaba yi-HEARDIN, i-sodium citrate noma i-Edta anticoaagulant plasma.

I-4.According kumasu ajwayelekile ukuqoqa isampula. Isampula ye-serum noma ye-plasma ingagcinwa esiqandisini ku-2-8 ① izinsuku ezi-3 kanye ne-cropresseving ngezansi -15 ° C izinyanga ezi-3.

I-DEALL GRACK GRAYZE-TRAWE-THAW CYCLELES.