Isimiso

Ulwelwesi lwedivayisi yokuhlola luhlanganiswe ne-anti FPSA antinond esifundeni sokuhlola kanye nembuzi anti Unogwaja I-IGG antibody on the control. I-Lable Pad ihlanganiswe yi-fluorescence ilebula anti psa antibody kanye ne-rabbit igg kusengaphambili. Lapho uhlola isampula elinethemba, i-FPSA Antigen yesampula ihlangana ne-fluorescence ilebula antibody antibody, bese wenza umxube wokuzivikela. Ngaphansi kwesenzo se-Immunochrotography, ukugeleza okuyinkimbinkimbi kokuqondiswa kwephepha lokumunca, lapho kuyinkimbinkimbi kudlula esifundeni sokuhlola, kuhlanganiswe ne-anti FPSA coating antilody, amafomu amasha abucayi. Izinga le-FPSA lixhumeka kahle ngesiginali ye-fluorescence, kanye ne-FPPSA ngesampula yesampula ingatholwa yi-fluorescence immunoassay assay.

Inqubo yokuhlola:

Uyacelwa ukuthi ufunde incwadi yokusebenza kwensimbi yokusebenza kwensimbi kanye nokufaka iphakheji ngaphambi kokuhlolwa.

1. Beka eceleni yonke imiphenduli namasampula wokushisa kwegumbi.
2. Vula i-Immune Analyzer ye-Immunezer (Wiz-A101), faka ukungena kwephasiwedi ye-akhawunti ngokuya ngendlela yokusebenza yensimbi, bese ufaka isikhombimsebenzisi sokuthola.
3. Skena ikhodi yamazinyo ukuze uqinisekise into yokuhlola.
4. Thatha ikhadi lokuhlola esikhwameni se-foil.
5 Faka ikhadi lokuhlola ku-slot yekhadi, scan ikhodi ye-QR, bese unquma into yokuhlola.
I-6. Faka i-40μL serum noma isampula ye-plasma ibe yi-dilount esampule, uhlanganise kahle.
7. Faka isixazululo sesampuli esingu-80μL ukuze isampula kahle ekhadini.
8. Chofoza inkinobho ethi "Ukuhlolwa Okujwayelekile", ngemuva kwemizuzu engu-15, ithuluzi lizobona ngokuzenzakalelayo ikhadi lokuhlola, lingafunda imiphumela kusuka esikrinini sokubonisa sensimbi, bese uqopha / phrinta imiphumela yokuhlola.
9. Bheka imiyalo ye-Immune Analyzer ephathekayo (Wiz-A101).