Isimiso

Ulwelwesi lwedivayisi yokuhlola luhlanganiswe ne-Fob antibody kwisifunda sokuhlola kanye nembuzi anti Unogwaja IGG antibody on the control. I-LAble Pad ihlanganiswe yi-fluorescence ilebula anti fob antibody kanye ne-rabbit igg kusengaphambili. Lapho uhlola isampula elinethemba, i-FOB Antigen yesampula yokuhlangana ne-fluorescence ilebula anti endody antibody, bese wenza umxube wokuzivikela. Ngaphansi kwesenzo se-Immunochrotography, ukugeleza okuyinkimbinkimbi kokuqondiswa kwephepha lokumunca. Lapho kuyinkimbinkimbi edlula esifundeni sokuhlola, kuhlanganiswe ne-anti fob coating antipod antibod, amafomu amasha. Uma kungalungile, isampula ayinayo i-hemoglobin yomuntu noma okuqukethwe kuphansi, izakhiwo ezanele zokuzivikela azinakuvikeleka. Ngeke kube khona umugqa obomvu endaweni yokutholwa (T). Umugqa obomvu yilezi zindlela ezivela endaweni yokulawula ikhwalithi (C) yokwahlulela ukuthi ngabe kukhona amasampula anele nokuthi inqubo ye-chromatomatography ijwayelekile. Iphinde isetshenziswe njengezinga lokulawula langaphakathi lama-reagents.

Inqubo yokuhlola:

Sicela ufunde ukufaka iphakethe ngaphambi kokuhlolwa.

1. Lawula ikhadi lokuhlola kusuka esikhwameni se-foil, ulibeke etafuleni lezinga bese umaka.

2.Remove cap kusuka kushubhu eyisampula futhi ulahle amasampula amabili okuqala ahlanjululwe, engeza amaconsi ama-3 (cishe u-100Ul) Ayikho Isampula evuswe nge-Bubble ngokuqondile futhi kancane kancane ekhadini elinikezwe ngekhadi elinikeziwe. Bese uqala isibali sikhathi.

3.Umphumela kufanele ufundwe kungakapheli imizuzu eyi-10-15, futhi akulungile ngemuva kwemizuzu engu-15.