ISIMISO

I-membrane yedivayisi yokuhlola imbozwe nge-FOB antibody endaweni yokuhlola kanye ne-anti-rabbit IgG ye-antibody yembuzi endaweni yokulawula. Iphedi elilebula limbozwa nge-fluorescence ebhalwe ukuthi i-anti FOB antibody kanye ne-rabbit IgG kusengaphambili. Lapho kuhlolwa isampula elihle, i-antigen ye-FOB kusampula ihlangana ne-fluorescence ebhalwe ukuthi i-anti FOB antibody, bese yenza ingxube yamasosha omzimba. Ngaphansi kwesenzo se-immunochromatography, ukugeleza okuyinkimbinkimbi kubheke ephepheni elimuncayo. Lapho inkimbinkimbi idlula isifunda sokuhlola, ihlanganiswe ne-anti-FOB yokuhlanganisa i-antibody, yakha inkimbinkimbi entsha. Uma inegethivu, isampula ayinayo i-hemoglobin yomuntu noma okuqukethwe kuphansi, ama-immune complexes anele awakwazi ukwakhiwa. Ngeke kube khona umugqa obomvu endaweni yokuthola (T). Ulayini obomvu yizinga elivela endaweni yokulawula ikhwalithi (C) ukuze kuhlungwe ukuthi akhona yini amasampula anele nokuthi inqubo ye-chromatography ijwayelekile yini. Iphinde isetshenziswe njengezinga lokulawula langaphakathi lama-reagents.

Inqubo Yokuhlola:

Sicela ufunde okufakiwe kwephakheji ngaphambi kokuhlola.

1.Khipha ikhadi lokuhlola esikhwameni se-foil, ulibeke etafuleni lezinga bese ulimaka.

2.Khipha ikepisi kushubhu yesampula bese ulahla amathonsi amabili okuqala esampula ehlanjululiwe, engeza amaconsi ama-3 (cishe u-100uL) alikho ibhamuza elihlanjululwe isampula liqonde phezulu futhi kancane ungene emthonjeni wesampula wekhadi eline-dispette enikeziwe. Bese uqala isibali sikhathi.

3.Umphumela kufanele ufundwe phakathi kwemizuzu eyi-10-15, futhi awuvumelekile ngemuva kwemizuzu eyi-15.