ISIMISO

Ulwelwesi lwedivayisi yokuhlola lumbozwe ngamasosha omzimba e-CRP endaweni yokuhlola kanye ne-anti-rabbit IgG yamasosha omzimba endaweni yokulawula. Iphedi elilebula limbozwa nge-fluorescence ebhalwe ukuthi anti-CRP antibody kanye ne-rabbit IgG kusengaphambili. Lapho kuhlolwa isampula elihle, i-antigen ye-CRP kusampula ihlangana ne-fluorescence ebhalwe ukuthi i-anti-CRP antibody, bese yenza ingxube yamasosha omzimba. Ngaphansi kwesenzo se-immunochromatography, ukugeleza okuyinkimbinkimbi kubheke ephepheni elimuncayo, lapho inkimbinkimbi idlula isifunda sokuhlola, ihlanganiswe ne-anti-CRP yokuhlanganisa i-antibody, yakha inkimbinkimbi entsha. Izinga le-CRP lihlotshaniswa kahle nesignali ye-fluorescence, futhi ukugxila kwe-CRP kusampula kungatholwa nge-fluorescence immunoassay assay.

Inqubo Yokuhlola

Sicela ufunde imanuwali yokusebenza kwezinsimbi kanye nokufakwa kwephakheji ngaphambi kokuhlola.

1. Beka eceleni wonke ama-reagents namasampuli ekamelweni lokushisa.
2. Vula i-Portable Immune Analyzer(WIZ-A101), faka ukungena ngemvume kwephasiwedi ye-akhawunti ngokwendlela yokusebenza yensimbi, bese ufaka isixhumi esibonakalayo sokubona.
3. Skena ikhodi yokuhlonza ukuze uqinisekise into yokuhlola.
4. Khipha ikhadi lokuhlola esikhwameni se-foil.
5. Faka ikhadi lokuhlola endaweni yekhadi, skena ikhodi ye-QR, bese unquma into yokuhlola
6. Engeza i-5μL ye-serum/isampula ye-plasma (noma 10μL Igazi Eliphelele) kwisampula ye-diluent, bese uxuba kahle.
7. Engeza isisombululo sesampula esingu-80μL ukuze uthole isampula yomthombo wekhadi.
8. Chofoza inkinobho ethi "ukuhlolwa okujwayelekile", ngemva kwemizuzu engu-3, ​​ithuluzi lizothola ngokuzenzakalelayo ikhadi lokuhlola, lingakwazi ukufunda imiphumela esikrinini sokubonisa sensimbi, futhi lirekhode/liphrinte imiphumela yokuhlolwa.
9. Bheka imiyalelo ye-Portable Immune Analyzer(WIZ-A101).