I-Tf itholakala ikakhulukazi ku-plasma, isilinganiso sokuqukethwe singaba ngu-1.20 ~ 3.25g/L. Kubantu abanempilo indle, cishe abukho. Lapho umgudu wokugaya ukudla ukopha, i-Tf ku-serum igeleza ingene emgudwini wamathumbu futhi ikhishwe ngendle, igcwele indle yeziguli ezopha emathunjini. Ngakho-ke, i-fecal Tf idlala indima edingekayo nebalulekile ukuze kutholwe ukopha kwamathumbu. Ikhithi iwukuhlola kwekhwalithi olula, okubukwayo okuthola i-Tf endle yomuntu, inokuzwela okuphezulu kanye nokucaciswa okuqinile. Ukuhlolwa okusekelwe kumgomo wokusabela wesangweji wama-antibodies aphindwe kabili kanye nobuchwepheshe bokuhlaziya i-immunochromatographic assay, kunganikeza umphumela phakathi nemizuzu eyi-15.