Ikhithi yokuxilonga ye-Triodothyronine ephelele(Fleorescence Immunochrographic Assay)
Ngokusetshenziswa kokuxilongwa kwe-vitro kuphela
Uyacelwa ukuthi ufunde leli phakethe Faka ngokucophelela ngaphambi kokusebenzisa futhi ulandele imiyalo ngokuqinile. Ukwethembeka kwemiphumela ye-Assay akunakuqinisekiswa uma kukhona ukuphambuka emiyalweni ekuleW kufakiwe.

Ukusetshenziswa okuhlosiwe
Ikhithi yokuxilonga ye-TriodeTothyronine (Fleorescence Immunochrographic Assay i-Fluorescochrogragraphic Assay for the auditative detection ukuthola umsebenzi we-triodoothyronine (TT3) kwi-plasma, okusetshenziselwa ukunqunywa kwe-auxilis kumele kuqinisekiswe ezinye izindlela. Lokhu kuhlola kuhloselwe ukusetshenziswa kwezempilo kuphela.

Ukubeka kafushane
I-TriodothyYronine (T3) Isisindo Molecular 651D. Yindlela eyinhloko esebenzayo ye-thyroid hormone. Ingqikithi ye-T3 (i-T3, i-TT3) e-serum ihlukaniswe ngezinhlobo ezibophayo nezikhululekile. I-99.5% ye-TT3 BINDS to Serum throwroxine amaprotheni amaprotheni (TBP), kanye nama-T3 (ama-T3 wamahhala we-T3) angu-0,2 kuya ku-0.4%. I-T4 ne-T3 ibambe iqhaza ekulondolozeni nasekulaleni i-metabolic function of metabolic function.TT3 kusetshenziselwa ukuhlola isimo sokusebenza kwe-thyroid nokuxilongwa kwezifo. I-Clinical TT3 iyinkomba ethembekile yokuxilongwa kanye nokubhekwa kokusebenza kwempumelelo kwe-hyperthyroidism ne-hypothyroidism.Ukuthobeka kwe-T3 kubaluleke kakhulu ekuhlolweni kwe-hyperthyroidism kune-T4.

Umgomo wenqubo

Ulwelwesi lwedivayisi yokuhlola luhlanganiswe ne-conjugate ye-BSA ne-T3 esifundeni sokuhlola kanye nembuzi anti Unogwaja IGG antibody on the control. I-Marker Pad ihlanganiswe ngu-Fluorescence Mark anti T3 antibody kanye noRabbit IGG kusengaphambili. Lapho uhlola isampula, i-TT3 ngesampula ihlangana ne-fluorescence imakwe uphawu lwe-anti t3 antibody, bese wenza umxube wokuzivikela. Ngaphansi kwesenzo se-Immunochrotography, ukugeleza okuyinkimbinkimbi kokuqondisa kwephepha lokungena, lapho i-Free Fluorescent Marker izohlanganiswa ne-T3 ku-TT3 ku-TT3 okulungile kwesiginali ye-fluorescence, kanye Ukuhlushwa kwe-TT3 ngesampula kungatholwa yi-fluorence iMmunoassay Assay.

Ama-reagents nezinto zokwenziwa

25T Iphakethe Izakhi:
.Test Card ngakunye i-foil ipaniwe nge-desiccant 25t
.A Isixazululo 25t
.B Isixazululo 1
.Fackage Faka 1

Izinto ezidingekayo kodwa azinikeziwe
Isithakongo esiqoqiwe sesampula, isibali sikhathi

Ukuqoqwa kwesampula nesitoreji
1.Izimpumu ezihlolwe zingaba yi-serum, i-heparin anticoaagulant plasma noma i-edta anticoaagulant plasma.
2.Faka kumasu ajwayelekile ukuqoqa isampula. Isampula ye-serum noma ye-plasma ingagcinwa esiqandisini ku-2-8 ℃ ngezinsuku eziyi-7 days kanye ne-cropressoal
I-3.all isampula igweme imijikelezo ye-freeze-thaw.

Inqubo yokufa
Inqubo yokuhlola ithuluzi ibona incwajana ye-immunoanalyzer. Inqubo yokuhlola i-Reagent imi kanjena
1.Lay ubeka wonke ama-reagents namasampula wokushisa kwegumbi.
I-2.open them immune analyzer (Wiz-A101), faka ukungena ngemvume kwe-akhawunti ngokusho kwendlela yokusebenza yensimbi, bese ufaka isikhombimsebenzisi sokuthola.
I-3.Sc Ikhodi Yokucacisa Ukuqinisekisa Into Yokuhlola.
4.Thola ikhadi lokuhlola esikhwameni se-foil.
5.Shuma ikhadi lokuhlola ekhadini lekhadi, scan ikhodi ye-QR, bese unquma into yokuhlola.
I-6.Add 30μL serum noma isampula ye-plasma kwikhambi, uhlanganise kahle.
7.ADD 20μL B Isixazululo sengxube engenhla, uhlanganise kahle.
8.Ama-ingxube imizuzu engama-20.
9.Add 80μl ingxube yesampula kahle ekhadini.
10.Chofoza inkinobho ethi "Ukuhlolwa okujwayelekile", ngemuva kwemizuzu eyi-10, ithuluzi lizothola ngokuzenzakalelayo ikhadi lokuhlola, lingafunda imiphumela kusuka esikrinini sokubonisa sensimbi, bese uqopha / phrinta imiphumela yokuhlola.
11.Refer ekufundisweni kwe-immune analyzer (Wiz-A101).

Amanani alindelwe

I-TT3 Range Ejwayelekile: 0.5-2.5ng / ML
Kunconywa ukuthi ilebhu ngalinye ngalinye lasungulwa uhla lwalo olujwayelekile olumele abantu balo.

Imiphumela yokuhlola nokutolika
.Imininingwane engenhla iyisikhashana esisungulwe idatha yokutholwa kwale kit, futhi kuphakanyiswa ukuthi ilabhorethri ngalinye kufanele lisungule isikhawu sokubaluleka komtholampilo.
.Ukuhluzwa kwe-TT3 kuphakeme kunebanga lezethenjwa, kanye nezinguquko zomzimba noma impendulo yokucindezela kufanele ikhishwe ngaphandle kwangajwayelekile.
.Imiphumela yale ndlela isebenza kuphela ebangeni lereferensi elisungulwe yile ndlela, futhi imiphumela ayiqhathaniswa ngqo nezinye izindlela.
Izici ze-.

Ukugcinwa nokuqina
I-1.Ikhithi iyizinyanga eziyi-18 zeShelf-Life kusuka ngosuku lokukhiqiza. Gcina amakhithi angasetshenziswanga ngo-2-30 ° C. Ungabazi. Ungasebenzisi ngaphezu kosuku lokuphelelwa yisikhathi.
2.Ungakuvuli esikhwameni esivaliwe uze ulungele ukwenza isivivinyo, futhi ukuhlolwa okusetshenziswa okukodwa kuphakanyiswa ukuthi kusetshenziswe ngaphansi kwendawo edingekayo (lokushisa 2-35 ℃, umswakama we-40-90%) ngaphakathi kwamaminithi angama-60 ngokushesha ngangokunokwenzeka.
3.Sample Diloulent isetshenziswa ngokushesha ngemuva kokuvulwa.

Izexwayiso kanye nokuqapha
.Ikhithi kufanele ivalwe futhi ivikelwe kumswakama.
.Imininingwane emihle izoqinisekiswa ezinye izindlela.
.All amasampula azophathwa njengokungcoliswa okungaba khona.
.Ungasebenzisi i-reagent ephelelwe yisikhathi.
.Danga ukuguqula ama-reagents phakathi kwama-kits ane-lot ehlukile cha ..
.Ungasebenzisi kabusha amakhadi okuhlola nanoma yiziphi izesekeli ezilahlwayo.
.Kungani, isampula ngokweqile noma elincane lingaholela ekuphaphemeni komphumela.

LUkufanisa
.S nganoma yisiphi ama-antibodies asegulayo e-Assay asebenzisa, kungenzeka ukuthi kuphazanyiswe ngama-antibodies angomuntu alwa nobuntu (Hama) ku-specimen. Izinhlobo ezivela ezigulini ezithole amalungiselelo ama-antibodies e-monoclonal yokuxilongwa noma ukwelashwa kungaqukatha uHama. Izinhlobo ezinjalo zingadala imiphumela emibi noma engamanga.
Umphumela wokuhlola we-.Lokhuwo we-. .
.Lokhu i-realent isetshenziselwa kuphela ukuhlolwa kwe-serum ne-plasma. Kungase kungatholi umphumela onembile uma usetshenziselwa amanye amasampula anjenge-saliva nomchamo nokunye.

Izici Zokusebenza

REcabanga
1.Hansen JH, et al.hama ukuphazamiseka nge-murine monoclonal antibody-based immunody [j] .j we-Clin immunoassay, 1993,16: 294-299.
I-2.levinson ss.Imvelo yama-antibophilies ama-heterophilic kanye neqhaza ekuphazanyisweni kwe-immunoassay [j] .j we-mimunoassay, 1992,15: 108-114.

Ukhiye wezimpawu ezisetshenzisiwe:

	Kudivayisi yezokwelapha yokuxilongwa kwe-vitro
	Umenzi
	Gcina ngo-2-30 ℃
	Usuku lokuphelelwa isikhathi
	Ungaphinde usebenzise kabusha
	Isiqapheliso
	Bheka imiyalo yokusebenzisa

Xiamen Wiz Biotech Co., Ltd
Ikheli: 3-4 phansi, i-No.16 yokwakha, indawo yokusebenzela ye-bio-medical, 2030 wengjiao West Road, Haicang District, 3610266, xiamen, China
I-TELE: + 86-592-6808278
Ifeksi: + 86-592-6808279