Ikhithi yokuxilonga yeTotal Thyroxine (ukuhlolwa kwe-fluorescence immunochromatographic)
Ukusetshenziswa kwe-in vitro diagnostic kuphela
Sicela ufunde le phakheji ngokucophelela ngaphambi kokuyisebenzisa futhi ulandele ngokuqinile imiyalelo. Ukuthembeka kwemiphumela yokuhlolwa akunakuqinisekiswa uma kukhona ukuchezuka emiyalweni ekule kokufakwa kwephakheji.

UKUSETSHENZISWA OKUHLOSIWE
Ikhithi yokuxilonga ye-Total Thyroxine (i-fluorescence immunochromatographic assay) iwukuhlolwa kwe-fluorescence immunochromatographic ukuze kutholwe inani le-Total Thyroxine (TT4) ku-serum yomuntu noma ku-plasma, esetshenziselwa kakhulu ukuhlola ukusebenza kwegilo. kufanele kuqinisekiswe ezinye izindlela. Lokhu kuhlolwa kuhloselwe ukusetshenziswa ngochwepheshe bezempilo kuphela.

ISIFINYEZO
I-Thyroxine(T4) ikhiqizwa indlala yegilo futhi isisindo sayo samangqamuzana angama-777D. Isamba se-T4(Ingqikithi T4,TT4) ku-serum iphinda izikhathi ezingama-50 kuneserum T3. Phakathi kwazo, u-99.9 % we-TT4 ubophezela ku-serum I-Thyroxine Binding Proteins(TBP), kanye ne-T4(Free T4,FT4) yamahhala ingaphansi kuka-0.05 %. I-T4 ne-T3 ibamba iqhaza ekulawuleni umsebenzi womzimba. Izilinganiso ze-TT4 zisetshenziselwa ukuhlola isimo sokusebenza kwe-thyroid kanye nokuxilongwa kwezifo. Ngokomtholampilo, i-TT4 iyinkomba ethembekile yokuxilongwa nokusebenza ngempumelelo kwe-hyperthyroidism kanye ne-hypothyroidism.

ISIMISO SENKQUBO
I-membrane yedivayisi yokuhlola ihlanganiswe ne-conjugate ye-BSA ne-T4 endaweni yokuhlola kanye ne-anti-rabbit IgG ye-antibody yembuzi endaweni yokulawula. Iphedi lomaka limbozwe nge-fluorescence mark anti-T4 antibody kanye ne-rabbit IgG kusengaphambili. Lapho kuhlolwa isampula, i-TT4 kusampula ihlangana ne-fluorescence ephawulwe ukuthi i-anti T4 antibody, bese yenza ingxube yokuzivikela komzimba. Ngaphansi kwesenzo se-immunochromatography, ukugeleza okuyinkimbinkimbi ekuqondeni kwephepha elimuncayo, lapho i-complex idlula indawo yokuhlola, Umaka we-fluorescent wamahhala uzohlanganiswa ne-T4 kulwelwesi. ukugxila kwe-TT4 kusampula kungatholwa ngokuhlolwa kwe-fluorescence immunoassay.

AMA-REAGENTS KANYE NEZINTO EZINIKEZELWAYO

25T izingxenye zephakheji:
.Iphepha lokuhlola ngalinye elifakwe esikhwameni nge-desiccant 25T
.Isixazululo 25T
.B isixazululo 1
.Faka iphakheji 1

IZINTO EZIDINGEKAYO KODWA AKUNIKEZWE
Isampula yokuqoqa isitsha, isibali sikhathi

UKUQOQWA KANYE NESIGCINISO ESAMPULA
1.Amasampula ahloliwe angaba i-serum, i-heparin anticoagulant plasma noma i-EDTA anticoagulant plasma.
2.Ngokwezinqubo ezijwayelekile qoqa isampula. I-Serum noma isampula ye-plasma ingagcinwa efrijini ku-2-8℃ izinsuku ezingu-7 kanye nokugcinwa kwe-cryopreservation ngaphansi -15°C izinyanga ezingu-6.
3.Wonke amasampula agwema imijikelezo yokuncibilika kweqhwa.

INQUBO YOKUHLOLA

Inqubo yokuhlola yensimbi bheka imanuwali ye-immunoanalyzer. Inqubo yokuhlola i-reagent imi kanje
1.Beka eceleni wonke ama-reagents namasampuli ekamelweni lokushisa.
2.Vula i-Portable Immune Analyzer(WIZ-A101), faka ukungena ngemvume kwephasiwedi ye-akhawunti ngokwendlela yokusebenza yethuluzi, bese ufaka isixhumi esibonakalayo sokubona.
3.Skena ikhodi yokuhlonza ukuze uqinisekise into yokuhlola.
3.Khipha ikhadi lokuhlola esikhwameni se-foil.
4.Faka ikhadi lokuhlola endaweni yekhadi, skena ikhodi ye-QR, bese unquma into yokuhlola.
5.Yengeza i-serum engu-20μL noma isampula ye-plasma ku-A, bese uxuba kahle.
6.Faka isixazululo esingu-20μL B kule ngxube engenhla, bese uxuba kahle.

Shiya ingxube ukuze20imizuzu.
Engeza ingxube engu-80μL ukuze usampula kahle ikhadi.
Chofoza inkinobho “yokuhlola okuvamile”, ngemva kwemizuzu eyi-10, ithuluzi lizothola ngokuzenzakalelayo ikhadi lokuhlola, lingakwazi ukufunda imiphumela esikrinini sokubonisa sensimbi, futhi lirekhode/liphrinte imiphumela yokuhlolwa.
Bheka imiyalelo ye-Portable Immune Analyzer(WIZ-A101).

IZIMALI EZILINDELEKILE
Ibanga elijwayelekile le-TT4:55-140nmol/L
Kutuswa ukuthi ilabhorethri ngayinye isungule ububanzi bayo obujwayelekile obumelela isiguli sayo.

IMIPHUMELA YOKUHLOLA NOKUTOLIZWA
.Le datha engenhla isikhawu sereferensi esisungulwe kudatha yokuthola yale khithi, futhi kuphakanyiswa ukuthi ilabhorethri ngayinye kufanele isungule isikhawu senkomba ngokubaluleka komtholampilo okufanelekile kwenani labantu kulesi sifunda.
.Ukuhlushwa kwe-TT4 kuphakeme kunohla lwereferensi, futhi izinguquko zomzimba noma impendulo yokucindezeleka kufanele ikhishwe.Ngempela okungavamile, kufanele kuhlanganise ukuhlonzwa kwezimpawu zomtholampilo.
.Imiphumela yale ndlela isebenza kuphela ebangeni lesithenjwa elisungulwe yile ndlela, futhi imiphumela ayiqhathanisi ngokuqondile nezinye izindlela.
.Ezinye izici zingaphinda zibangele amaphutha emiphumeleni yokuthola, okuhlanganisa izizathu zobuchwepheshe, amaphutha okusebenza nezinye izici zesampula.

UKUGCINWA NOKUZInza
.Ikhithi iyishelufu yezinyanga eziyi-18 kusukela ngosuku eyakhiwa ngalo. Gcina amakhithi angasetshenzisiwe ku-2-30°C. UNGAQISHI. Ungasebenzisi ngale kosuku lokuphelelwa yisikhathi.
.Ungavuli isikhwama esivaliwe kuze kube yilapho usulungele ukwenza isivivinyo, futhi ukuhlola ukusetshenziswa kanye kuyaphakanyiswa ukuthi kusetshenziswe ngaphansi kwendawo edingekayo (izinga lokushisa elingu-2-35℃, umswakamo 40-90%) phakathi kwemizuzu engu-60 ngokushesha kungenzeka.
.I-diluent eyisampula isetshenziswa ngokushesha ngemva kokuvulwa.

IZIXWAYISO NEZIQINISEKISO
.Ikhithi kufanele ivalwe futhi ivikelwe emswakameni.
.Zonke izibonelo ezinhle zizoqinisekiswa ngezinye izindlela.
.Zonke izifanekiso zizophathwa njengamandla angcolisayo.
.UNGAsebenzisi i-reagent ephelelwe yisikhathi.
.UNGAshintshisani ama-reagents phakathi kwamakhithi anenombolo ehlukile yenombolo..
.UNGAphinde usebenzise amakhadi okuhlola nanoma yiziphi izisekeli ezilahlwayo.
.Ukungasebenzi kahle, isampula eliningi noma elincane lingaholela ekuphambukeni kwemiphumela.

LUKULINGISA
.Njenganoma yikuphi ukulinganisa okusebenzisa amasosha omzimba egundane, kungenzeka ukuthi kuphazamiseke amasosha omzimba alwa namagundane (HAMA) esibonelweni. Izibonelo zeziguli ezithole amalungiselelo e-monoclonal antibodies ukuze zixilongwe noma zelashwe zingaqukatha i-HAMA. Izibonelo ezinjalo zingadala imiphumela engemihle noma engemihle engamanga.
.Lo mphumela wokuhlolwa ngowenkomba yomtholampilo kuphela, akufanele usebenze njengesisekelo sokuxilongwa nokwelashwa kweziguli, ukuphathwa komtholampilo kweziguli kufanele kucatshangelwe ngokugcwele kuhlanganiswe nezimpawu zayo, umlando wezokwelapha, okunye ukuhlolwa kwaselabhorethri, impendulo yokwelashwa, i-epidemiology kanye nolunye ulwazi. .
.Lesi siphehli sisetshenziswa kuphela ekuhlolweni kwe-serum ne-plasma. Ingase ingatholi umphumela onembile uma isetshenziselwa amanye amasampula afana namathe nomchamo nokunye.

IZIMPAWU ZOKUSEBENZA

REFERENCES
1.Hansen JH,et al.HAMA Ukuphazamisa Nge-Murine Monoclonal Antibody-Based Immunoassays[J].J ye-Clin Immunoassay,1993,16:294-299.
2.Levinson SS.Imvelo Yama-Heterophilic Antibodies kanye Neqhaza Ekuphazamisekeni Kwe-Immunoassay[J].J ye-Clin Immunoassay,1992,15:108-114.

Ukhiye wezimpawu ezisetshenzisiwe:

	In Vitro Diagnostic Medical Device
	Umkhiqizi
	Gcina ku-2-30 ℃
	Usuku lokuphelelwa isikhathi
	Ungaphinde Usebenzise
	ISEXWAYISO
	Bheka Imiyalo Yokusetshenziswa

I-Xiamen Wiz Biotech CO., LTD
Ikheli:3-4 Floor,NO.16 Building,Bio-medical Workshop,2030 Wengjiao West Road,Haicang District,361026,Xiamen,China
Ucingo: +86-592-6808278
Ifeksi:+86-592-6808279