I-Diagnostic Kit ye-Testosterone(ukuhlolwa kwe-fluorescence immunochromatographic)
Ukusetshenziswa kwe-in vitro diagnostic kuphela
Sicela ufunde le phakheji ngokucophelela ngaphambi kokuyisebenzisa futhi ulandele ngokuqinile imiyalelo. Ukuthembeka kwemiphumela yokuhlolwa akunakuqinisekiswa uma kukhona ukuchezuka emiyalweni ekule kokufakwa kwephakheji.

UKUSETSHENZISWA OKUHLOSIWE
I-Diagnostic Kit ye-Testosterone (i-fluorescence immunochromatographic assay) i-fluorescence immunochromatographic assay yokutholwa komthamo we-Testosterone (T) ku-serum yomuntu noma i-plasma, esetshenziselwa kakhulu ukuhlola amazinga e-Testosterone.Kuyi-axiliary diagnosis reagent.Zonke isampula enhle kufanele kuqinisekiswe ezinye izindlela. Lokhu kuhlolwa kuhloselwe ukusetshenziswa ngochwepheshe bezempilo kuphela.

ISIFINYEZO
I-Testosterone(T), enesisindo samangqamuzana esingu-288.4 D.Ezimisweni zokujikeleza kwegazi, cishe amaphesenti angama-97% kuya ku-99% e-testosterone abopha amaprotheni e-plasma. SHBG),38% kuboshelwe ku-albumin, kanti u-2% umahhala. I-testosterone ebophayo egazini ayisebenzi ngokwezinto eziphilayo, futhi i-testosterone yamahhala kuphela engangena kumaseli we-rake ukuze isebenzise imiphumela yayo yomzimba.

ISIMISO SENKQUBO
I-membrane yedivayisi yokuhlola ihlanganiswe ne-conjugate ye-BSA ne-Testosterone endaweni yokuhlola kanye ne-anti-rabbit ye-IgG yembuzi endaweni yokulawula. Iphedi lomaka limbozwe nge-fluorescence mark anti-Testosterone antibody kanye ne-rabbit IgG kusengaphambili. Lapho kuhlolwa isampula, i-Testosterone kusampula ihlangana ne-fluorescence ephawulwe ukuthi i-anti-Testosterone antibody, bese yenza ingxube yamasosha omzimba. Ngaphansi kwesenzo se-immunochromatography, ukugeleza okuyinkimbinkimbi ekuqondeni kwephepha elimuncayo, lapho i-complex idlula isifunda sokuhlola, Umaka we-fluorescent wamahhala uzohlanganiswa ne-Testosterone kulwelwesi. Ukuhlushwa kwe-Testosterone kusampula kungatholwa ngokuhlolwa kwe-fluorescence immunoassay.

AMA-REAGENTS KANYE NEZINTO EZINIKEZELWAYO

25T izingxenye zephakheji:
.Iphepha lokuhlola ngalinye elifakwe esikhwameni nge-desiccant 25T
.Isixazululo 25T
.B isixazululo 1
.Faka iphakheji 1

IZINTO EZIDINGEKAYO KODWA AKUNIKEZWE
Isampula yokuqoqa isitsha, isibali sikhathi

UKUQOQWA KANYE NESIGCINISO ESAMPULA
1.Amasampula ahloliwe angaba i-serum, i-heparin anticoagulant plasma noma i-EDTA anticoagulant plasma.
2.Ngokwezinqubo ezijwayelekile qoqa isampula. I-Serum noma isampula ye-plasma ingagcinwa efrijini ku-2-8℃ izinsuku ezingu-7 kanye nokugcinwa kwe-cryopreservation ngaphansi -15°C izinyanga ezingu-6.
3.Wonke amasampula agwema imijikelezo yokuncibilika kweqhwa.

INQUBO YOKUHLOLA
Inqubo yokuhlola yensimbi bheka imanuwali ye-immunoanalyzer. Inqubo yokuhlola i-reagent imi kanje
1.Beka eceleni wonke ama-reagents namasampuli ekamelweni lokushisa.
2.Vula i-Portable Immune Analyzer(WIZ-A101), faka ukungena ngemvume kwephasiwedi ye-akhawunti ngokwendlela yokusebenza yethuluzi, bese ufaka isixhumi esibonakalayo sokubona.
3.Skena ikhodi yokuhlonza ukuze uqinisekise into yokuhlola.
4.Khipha ikhadi lokuhlola esikhwameni se-foil.
5.Faka ikhadi lokuhlola endaweni yekhadi, skena ikhodi ye-QR, bese unquma into yokuhlola.
6.Faka i-30μL serum noma isampula ye-plasma ku-A, bese uxuba kahle.
7.Faka isixazululo esingu-20μL B kule ngxube engenhla, bese uxuba kahle.
8.Shiya ingxube imizuzu engu-20.
9.Yengeza ingxube engu-80μL ukuze usampula kahle ikhadi.
10.Chofoza inkinobho ethi “standard test”, emva kwemizuzu eyi-10, ithuluzi lizothola ngokuzenzakalelayo ikhadi lokuhlola, lingakwazi ukufunda imiphumela esikrinini esibonisiwe sethuluzi, futhi lirekhode/liphrinte imiphumela yokuhlolwa.
11.Bheka umyalelo we-Portable Immune Analyzer(WIZ-A101).

IZIMALI EZILINDELEKILE

Testosterone uhla evamile: Owesilisa: 2.5-10.5ng/mL
Owesifazane: 0.25-1.0ng/mL
Kutuswa ukuthi ilabhorethri ngayinye isungule ububanzi bayo obujwayelekile obumelela isiguli sayo.

IMIPHUMELA YOKUHLOLA NOKUTOLIZWA
.Le datha engenhla isikhawu sereferensi esisungulwe kudatha yokuthola yale khithi, futhi kuphakanyiswa ukuthi ilabhorethri ngayinye kufanele isungule isikhawu senkomba ngokubaluleka komtholampilo okufanelekile kwenani labantu kulesi sifunda.
.Ukuhlushwa kwe-Testosterone kuphakeme kunohla lwereferensi, futhi izinguquko zomzimba noma impendulo yokucindezeleka kufanele ikhishwe.Ngempela okungavamile, kufanele kuhlanganiswe ukuhlonza izimpawu zomtholampilo.
.Imiphumela yale ndlela isebenza kuphela ebangeni lesithenjwa elisungulwe yile ndlela, futhi imiphumela ayiqhathanisi ngokuqondile nezinye izindlela.
.Ezinye izici zingaphinda zibangele amaphutha emiphumeleni yokuthola, okuhlanganisa izizathu zobuchwepheshe, amaphutha okusebenza nezinye izici zesampula.

UKUGCINWA NOKUZInza
1.Ikhithi iyimpilo yeshelufu yezinyanga eziyi-18 kusukela ngosuku lokukhiqizwa kwayo. Gcina amakhithi angasetshenzisiwe ku-2-30°C. UNGAQISHI. Ungasebenzisi ngale kosuku lokuphelelwa yisikhathi.
2.Ungasivuli isikhwama esivaliwe kuze kube yilapho usulungele ukwenza isivivinyo, futhi ukuhlolwa okukodwa kuphakanyiswa ukuthi kusetshenziswe ngaphansi kwendawo edingekayo (izinga lokushisa elingu-2-35℃, umswakama 40-90%) phakathi kwemizuzu engama-60 ngokushesha. ngangokunokwenzeka.
I-3.I-diluent yesampula isetshenziswa ngokushesha ngemva kokuvulwa.

IZIXWAYISO NEZIQINISEKISO
.Ikhithi kufanele ivalwe futhi ivikelwe emswakameni.
.Zonke izibonelo ezinhle zizoqinisekiswa ngezinye izindlela.
.Zonke izifanekiso zizophathwa njengamandla angcolisayo.
.UNGAsebenzisi i-reagent ephelelwe yisikhathi.
.UNGAshintshisani ama-reagents phakathi kwamakhithi anenombolo ehlukile yenombolo..
.UNGAphinde usebenzise amakhadi okuhlola nanoma yiziphi izisekeli ezilahlwayo.
.Ukungasebenzi kahle, isampula eliningi noma elincane lingaholela ekuphambukeni kwemiphumela.

LUKULINGISA
.Njenganoma yikuphi ukulinganisa okusebenzisa amasosha omzimba egundane, kungenzeka ukuthi kuphazamiseke amasosha omzimba alwa namagundane (HAMA) esibonelweni. Izibonelo zeziguli ezithole amalungiselelo e-monoclonal antibodies ukuze zixilongwe noma zelashwe zingaqukatha i-HAMA. Izibonelo ezinjalo zingadala imiphumela engemihle noma engemihle engamanga.
.Lo mphumela wokuhlolwa ngowenkomba yomtholampilo kuphela, akufanele usebenze njengesisekelo sokuxilongwa nokwelashwa kweziguli, ukuphathwa komtholampilo kweziguli kufanele kucatshangelwe ngokugcwele kuhlanganiswe nezimpawu zayo, umlando wezokwelapha, okunye ukuhlolwa kwaselabhorethri, impendulo yokwelashwa, i-epidemiology kanye nolunye ulwazi. .
.Lesi siphehli sisetshenziswa kuphela ekuhlolweni kwe-serum ne-plasma. Ingase ingatholi umphumela onembile uma isetshenziselwa amanye amasampula afana namathe nomchamo nokunye.

IZIMPAWU ZOKUSEBENZA

IZIKHOMBISI
1.Hansen JH,et al.HAMA Ukuphazamisa Nge-Murine Monoclonal Antibody-Based Immunoassays[J].J ye-Clin Immunoassay,1993,16:294-299.
2.Levinson SS.Imvelo Yama-Heterophilic Antibodies kanye Neqhaza Ekuphazamisekeni Kwe-Immunoassay[J].J ye-Clin Immunoassay,1992,15:108-114.

Ukhiye wezimpawu ezisetshenzisiwe:

	In Vitro Diagnostic Medical Device
	Umkhiqizi
	Gcina ku-2-30 ℃
	Usuku lokuphelelwa isikhathi
	Ungaphinde Usebenzise
	ISEXWAYISO
	Bheka Imiyalo Yokusetshenziswa

I-Xiamen Wiz Biotech CO., LTD
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