I-Diagnostic Kit ye-progesterone(Fleorescence Immunochrographic Assay)
Ngokusetshenziswa kokuxilongwa kwe-vitro kuphela
Uyacelwa ukuthi ufunde leli phakethe Faka ngokucophelela ngaphambi kokusebenzisa futhi ulandele imiyalo ngokuqinile. Ukwethembeka kwemiphumela ye-Assay akunakuqinisekiswa uma kukhona ukuphambuka emiyalweni ekuleW kufakiwe.

Ukusetshenziswa okuhlosiwe
I-Diagnescence Kit ye-Progesteronone (Fleorescence Immunochrohamagraphic Assay iyinhlangano ye-fluoresrochrographic assay yokutholwa kwe-progesterone (prog) ku-progesterone izifo ezihlotshaniswayo. Lokhu kuhlola kuhloselwe ukusetshenziswa kwezempilo kuphela.

Ukubeka kafushane
I-Progesterone iyi-hormone ebalulekile edlala indima ebalulekile ekuqondisweni komjikelezo wokuya esikhathini futhi kubalulekile ekugcineni ukukhulelwa. Ukuhlushwa kwe-progesterone ku-serum kukhuphuke ngokushesha ngemuva kwe-ovulation. Kuyinkomba ethembekile ye-ovulation yemvelo noma ukungeniswa kwe-ovulation.

Umgomo wenqubo
Ulwelwesi lwedivayisi yokuhlola luhlanganiswe ne-conjugate ye-BSA kanye ne-prog esifundeni sokuhlola kanye nembuzi anti Unogwaja I-IGG antibody on the control. I-Marker Pad ihlanganiswe yi-fluorescence mark anti prog antibog antibog ne-iGG yoRabbit ngaphambi kwesikhathi. Lapho uhlola isampula, i-prog kusampula hlanganisa nge-fluorescence emakwe anti prog antibog antibog, bese wenza umxube wokuzivikela. Ngaphansi kwesenzo se-Immunochrotography, ukugeleza okuyinkimbinkimbi kwephepha lokumunca, lapho i-fluorescent maker ihlanganiswe ne-membrane.

Ama-reagents nezinto zokwenziwa
25T Iphakethe Izakhi:
Ikhadi lokuhlola ngawodwana ahlelwe nge-desiccant 25t
Isampula dilients 25t
Faka iphakheji 1

Izinto ezidingekayo kodwa azinikeziwe
Isithakongo esiqoqiwe sesampula, isibali sikhathi

Ukuqoqwa kwesampula nesitoreji
1.Izimpumu ezihlolwe zingaba yi-serum, i-heparin anticoaagulant plasma noma i-edta anticoaagulant plasma.
2.Faka kumasu ajwayelekile ukuqoqa isampula. Isampula ye-serum noma ye-plasma ingagcinwa esiqandisini ku-2-8 ℃ ngezinsuku eziyi-7 days kanye ne-cropressoal
I-3.all isampula igweme imijikelezo ye-freeze-thaw.

Inqubo yokufa
Uyacelwa ukuthi ufunde incwadi yokusebenza kwensimbi yokusebenza kwensimbi kanye nokufaka iphakheji ngaphambi kokuhlolwa.
1.Lay ubeka wonke ama-reagents namasampula wokushisa kwegumbi.
2Ngoba i-Immune Analyzer ye-Immunezer (Wiz-A101), faka ukungena ngemvume kwe-akhawunti ngokusho kwendlela yokusebenza yensimbi, bese ufaka isikhombimsebenzisi sokuthola.
I-3.Sc Ikhodi Yokucacisa Ukuqinisekisa Into Yokuhlola.
4.Thola ikhadi lokuhlola esikhwameni se-foil.
5.Shuma ikhadi lokuhlola ekhadini lekhadi, scan ikhodi ye-QR, bese unquma into yokuhlola.
I-6.Add 20μL serum noma isampula ye-plasma ukuze isampula isampula, uhlanganise kahle ..
7.ADD 80μL isixazululo isampula yesampula kahle yekhadi.
8.Chofoza inkinobho ethi "Ukuhlolwa okujwayelekile", ngemuva kwemizuzu eyi-10, ithuluzi lizobona ngokuzenzakalelayo ikhadi lokuhlola, lingafunda imiphumela kusuka esikrinini sokubonisa sensimbi, bese uqopha / phrinta imiphumela yokuhlola.
9.Refer ekufundisweni kwe-immune analyzer (Wiz-A101).

Imiphumela yokuhlola nokutolika

.Imininingwane engenhla ingumphumela wokuhlolwa kwe-reagent ye-prog, futhi kuphakanyiswa ukuthi ilabhorethri ngalinye kufanele lisungule uhla lwamanani wokutholwa kwe-prog elungele inani labantu kulesi sifunda. Imiphumela engenhla ingeyokubhekiswa kuphela.
.Imiphumela yale ndlela isebenza kuphela kumabanga okuthola amareferensi asungulwe kule ndlela, futhi akukho ukuqhathanisa okuqondile nezinye izindlela.
Izici ze-.

Ukugcinwa nokuqina
I-1.Ikhithi iyizinyanga eziyi-18 zeShelf-Life kusuka ngosuku lokukhiqiza. Gcina amakhithi angasetshenziswanga ngo-2-30 ° C. Ungabazi. Ungasebenzisi ngaphezu kosuku lokuphelelwa yisikhathi.
2.Ungakuvuli esikhwameni esivaliwe uze ulungele ukwenza isivivinyo, futhi ukuhlolwa okusetshenziswayo okukodwa kuphakanyiswa ukuthi kusetshenziswe ngaphansi kwendawo edingekayo (lokushisa 2-35 ℃, umswakama u-40-90%) ngaphakathi kwamaminithi angama-60 ngokushesha okukhulu.
I-3Sample Dileunt isetshenziswa ngokushesha ngemuva kokuvulwa.

Izexwayiso kanye nokuqapha
.Ikhithi kufanele ivalwe futhi ivikelwe kumswakama.
.Imininingwane emihle izoqinisekiswa ezinye izindlela.
.All amasampula azophathwa njengokungcoliswa okungaba khona.
.Ungasebenzisi i-reagent ephelelwe yisikhathi.
.Danga ukuguqula ama-reagents phakathi kwama-kits ane-lot ehlukile cha ..
.Ungasebenzisi kabusha amakhadi okuhlola nanoma yiziphi izesekeli ezilahlwayo.
.Kungani, isampula ngokweqile noma elincane lingaholela ekuphaphemeni komphumela.

LUkufanisa
.S nganoma yisiphi ama-antibodies asegulayo e-Assay asebenzisa, kungenzeka ukuthi kuphazanyiswe ngama-antibodies angomuntu alwa nobuntu (Hama) ku-specimen. Izinhlobo ezivela ezigulini ezithole amalungiselelo ama-antibodies e-monoclonal yokuxilongwa noma ukwelashwa kungaqukatha uHama. Izinhlobo ezinjalo zingadala imiphumela emibi noma engamanga.
Umphumela wokuhlola we-.Lokhuwo we-.Thizo kuphela kwisethenjwa somtholampilo kuphela, akufanele ukhonze njengeso sisekelo sokuxilongwa kokwelashwa nokwelashwa, ukuphathwa kweziguli kweziguli kufanele kube ukucatshangelwa okuphelele okuhlanganiswe nezimpawu zalo, umlando wezokwelapha, okunye ukuhlolwa kwelebhu, ukuphendula kokwelashwa, ukuphendula kokwelashwa, i-epidemiology kanye neminye imininingwane.
.Lokhu i-realent isetshenziselwa kuphela ukuhlolwa kwe-serum ne-plasma. Kungase kungatholi umphumela onembile uma usetshenziselwa amanye amasampula anjenge-saliva nomchamo nokunye.

Izici Zokusebenza

REcabanga
1.Hansen JH, et al.hama ukuphazamiseka nge-murine monoclonal antibody-based immunody [j] .j we-Clin immunoassay, 1993,16: 294-299.
I-2.levinson ss.Imvelo yama-antibophilies ama-heterophilic kanye neqhaza ekuphazanyisweni kwe-immunoassay [j] .j we-mimunoassay, 1992,15: 108-114.

Ukhiye wezimpawu ezisetshenzisiwe:

	Kudivayisi yezokwelapha yokuxilongwa kwe-vitro
	Umenzi
	Gcina ngo-2-30 ℃
	Usuku lokuphelelwa isikhathi
	Ungaphinde usebenzise kabusha
	Isiqapheliso
	Bheka imiyalo yokusebenzisa

Xiamen Wiz Biotech Co., Ltd
Ikheli: 3-4 phansi, i-No.16 yokwakha, indawo yokusebenzela ye-bio-medical, 2030 wengjiao West Road, Haicang District, 3610266, xiamen, China
I-TELE: + 86-592-6808278
Ifeksi: + 86-592-6808279