Isimiso:

Umucu une-anti-FOB enamathela amasosha omzimba endaweni yokuhlola, eboshelwe ku-membrane chromatography kusengaphambili. Iphedi elebula limbozwe i-fluorescence ebhalwe ukuthi i-anti-FOB antibody kusengaphambili. Lapho kuhlolwa isampula elihle, i-FOB kusampula ingaxutshwa ne-fluorescence ebhalwe ukuthi i-anti-FOB antibody, futhi yakhe inhlanganisela yokuzivikela komzimba. Njengoba ingxube ivunyelwa ukuthi ithuthe eduze komugqa wokuhlola, inkimbinkimbi ye-FOB conjugate ithathwa yi-anti-FOB enamathela kuma-antibody kulwelwesi futhi yakha inkimbinkimbi. Amandla e-fluorescence ahlotshaniswa kahle nokuqukethwe kwe-FOB. I-FOB esesampula ingatholwa nge-fluorescence immunoassay analyzer.

Inqubo Yokuhlola:

1.Beka eceleni wonke ama-reagents namasampuli ekamelweni lokushisa.
2.Vula i-Portable Immune Analyzer(WIZ-A101), faka ukungena ngemvume kwephasiwedi ye-akhawunti ngokwendlela yokusebenza yethuluzi, bese ufaka isixhumi esibonakalayo sokubona.
3.Skena ikhodi yokuhlonza ukuze uqinisekise into yokuhlola.
4.Khipha ikhadi lokuhlola esikhwameni se-foil.
5.Faka ikhadi lokuhlola endaweni yekhadi, skena ikhodi ye-QR, bese unquma into yokuhlola.
6.Khipha ikepisi kushubhu yesampula bese ulahla amathonsi amabili okuqala esampula ehlanjululiwe, engeza amaconsi ama-3 (cishe u-100uL) alikho ibhamuza elihlanjululwe isampula lime mpo futhi kancane ungene emthonjeni wesampula wekhadi eline-dispette enikeziwe.
7.Chofoza inkinobho ethi "standard test", ngemva kwemizuzu engu-15, ithuluzi lizobona ngokuzenzakalelayo ikhadi lokuhlola, lingakwazi ukufunda imiphumela esikrinini sokubonisa sensimbi, futhi lirekhode/liphrinte imiphumela yokuhlolwa.
8.Bheka umyalelo we-Portable Immune Analyzer(WIZ-A101).