Ikhithi yokuxilonga ye-Urine microalbumin

(I-Fluorescence Immunochromatographic Assay)

Ukusetshenziswa kwe-in vitro diagnostic kuphela

Sicela ufunde le phakheji ngokucophelela ngaphambi kokuyisebenzisa futhi ulandele ngokuqinile imiyalelo. Ukuthembeka kwemiphumela yokuhlolwa akunakuqinisekiswa uma kukhona ukuchezuka emiyalweni ekule kokufakwa kwephakheji.

UKUSETSHENZISWA OKUHLOSIWE

I-Diagnostic Kit for Urine microalbumin (i-Fluorescence Immunochromatographic Assay) ifaneleka ekutholweni kobuningi be-microalbumin emchamweni womuntu nge-fluorescence immunochromatographic assay, esetshenziselwa ikakhulukazi ukuxilongwa okuwusizo kwesifo sezinso.Wonke amasampula amahle kufanele aqinisekiswe ngezinye izindlela. Lokhu kuhlolwa kuhloselwe ukusetshenziswa ngochwepheshe bezempilo kuphela.

ISIFINYEZO

I-Microalbumin iphrotheni evamile etholakala egazini futhi iyivelakancane kakhulu emchamweni lapho igaywa ngokujwayelekile. Uma kukhona i-trace amount kumchamo we-Albumin engaphezu kuka-20 micron/mL, ingeye-microalbumin yomchamo, uma kungaba ukwelashwa okufika ngesikhathi, ingalungisa ngokuphelele i-glomeruli, iqede i-proteinuria, uma kungenjalo ukwelashwa okufika ngesikhathi, ingangena esigabeni se-uremia. I-microalbumin yomchamo ibonakala kakhulu ku-nephropathy yesifo sikashukela, umfutho wegazi ophakeme kanye ne-preeclampsia ekukhulelweni. Lesi simo singatholwa ngokunembile ngenani le-microalbumin yomchamo, kuhlanganiswe nesigameko, izimpawu kanye nomlando wezokwelapha. Ukutholwa kusenesikhathi kwe-microalbumin yomchamo kubaluleke kakhulu ukuvimbela kanye nokubambezela ukukhula kwe-nephropathy yesifo sikashukela.

ISIMISO SENKQUBO

Ulwelwesi lwedivayisi yokuhlola lumbozwe nge-ALB antigen endaweni yokuhlola kanye ne-anti-rabbit IgG ye-antibody yembuzi endaweni yokulawula. Iphedi lomaka limbozwe ngophawu lwe-fluorescence anti anti ALB kanye ne-rabbit IgG kusengaphambili. Lapho kuhlolwa isampula, i-ALB kusampula ihlangana ne-fluorescence ephawulwe ukuthi i-anti-ALB antibody, bese yenza ingxube yokuzivikela komzimba. Ngaphansi kwesenzo se-immunochromatography, ukugeleza okuyinkimbinkimbi kuya ohlangothini lwephepha elimuncayo, lapho i-complex idlula indawo yokuhlola, Umaka we-fluorescent wamahhala uzohlanganiswa ne-ALB kulwelwesi. Ukugxila kwe-ALB kusampula kungatholwa ngokuhlolwa kwe-fluorescence immunoassay.

AMA-REAGENTS KANYE NEZINTO EZINIKEZELWAYO

25T izingxenye zephakheji:

Ikhadi lokuhlola i-foil ngayinye efakwe esikhwameni se-desiccant 25T

Faka iphakheji 1

IZINTO EZIDINGEKAYO KODWA AKUNIKEZWE

Isampula yokuqoqa isitsha, isibali sikhathi

UKUQOQWA KANYE NESIGCINISO ESAMPULA

1. Amasampula ahloliwe angaba umchamo.
2. Amasampula omchamo amasha angaqoqwa esitsheni esihlanzekile esilahlwayo. Kunconywa ukuhlola amasampula omchamo ngokushesha ngemva kokuqoqwa. Uma amasampula omchamo engakwazi ukuhlolwa ngokushesha, sicela uwagcine ku-2-8℃, kodwa kunconywa ukuthi ungagcinie isikhathi esingaphezu kwamahora ayi-12. Unganyakazisi isitsha. Uma kunenhlabathi phansi esitsheni, thatha i-supernatant ukuze uyihlole.
3. Wonke amasampuli agwema imijikelezo yokuncibilika kweqhwa.
4. Ncibilikisa amasampula ekamelweni lokushisa ngaphambi kokusetshenziswa.