Ikhithi yokuxilonga ye-isoenzyme MB ye-Creatine Kinase(Fleorescence Immunochrographic Assay)
Ngokusetshenziswa kokuxilongwa kwe-vitro kuphela
Uyacelwa ukuthi ufunde leli phakethe Faka ngokucophelela ngaphambi kokusebenzisa futhi ulandele imiyalo ngokuqinile. Ukwethembeka kwemiphumela ye-Assay akunakuqinisekiswa uma kukhona ukuphambuka emiyalweni ekuleW kufakiwe.

Ukusetshenziswa okuhlosiwe
I-Diagnostic Kit ye-Isoenzyme MB ye-Creatine Kinase (Fleorescence Imunochrochrographic Assay i-Fluorescence Immunochrographic Assay for Creatine Kinase (CK-MB) ku-ADIne KINASE (CK-MB) e-Plasma, isetshenziselwa ukuxilongwa kwe-AMI (i-MyOCardial Infarction). Konke amasampula amahle kufanele kuqinisekiswe ezinye izindlela. Lokhu kuhlola kuhloselwe ukusetshenziswa kwezempilo kuphela.

Ukubeka kafushane
Amazinga we-CK-MB akhuphuke amahora angama-4-6 ngemuva kokuphazamiseka kwe-myocardial infarder, ephakanyiswe ngamahora angu-18- 24, futhi abuyela ezinsukwini ezingama-2-3 kamuva. Ukukhishwa kwe-CK-MB ekusakazweni ngemuva kwesikhathi kune-hemoglobin.ck-MB ngumakeke wendabuko we-infarction ecate myocardial infaction

Umgomo wenqubo
Ulwelwesi lwedivayisi yokuhlola luhlanganiswe ne-anti CK-MB antibody kwisifunda sokuhlola kanye nembuzi anti Unogwaja IGG Antibody on the Control Regibod on the Control Recindody on the Control Regiody on the Control Regibod on the Control Recindody on the Control Recindody on the Control Regiody on the Control Recindody on the Control Recindody on the Control Recindody on the Control Regibod on the Control Recindody on the Control Recindody on the Control Recindody on the Control Recindody on the Control Regiody on the Control Regibod on the Control Recindody on the Control Regibont on the Control Recindody. I-Lable Pad ihlanganiswe yi-fluorescence ilebula anti CK-MB antibody kanye noRabbit Igg ngaphambi kwesikhathi. Lapho uvivinya isampula elinethemba, i-CK-MB Antigen in Tample Combine ne-Fluorescence ilebula anti CK-MB antibody, bese wenza umxube we-immune. Ngaphansi kwesenzo se-Immunochrotography, ukugeleza okuyinkimbinkimbi kokuqondisa kwephepha lokumunca, lapho kuyinkimbinkimbi kudlula esifundeni sokuhlola, kuhlanganiswe ne-anti CK-MB ehlanganisa i-antibody, amafomu amasha. Ileveli ye-CK-MB ixhumeka kahle ngesiginali ye-fluorescence, kanye nokuhlushwa kwe-CK-MB ngesampula kungatholwa yi-fluorence iMmunoassay Assay.

Ama-reagents nezinto zokwenziwa

25T Iphakethe Izakhi:
Ikhadi lokuhlola ngawodwana ahlelwe nge-desiccant 25t
Isampula dilients 25t
Faka iphakheji 1

Izinto ezidingekayo kodwa azinikeziwe
Isithakongo esiqoqiwe sesampula, isibali sikhathi

Ukuqoqwa kwesampula nesitoreji
1.Izimpumu ezihlolwe zingaba yi-serum, i-heparin anticoaagulant plasma noma i-edta anticoaagulant plasma.
2.Faka kumasu ajwayelekile ukuqoqa isampula. Isampula ye-serum noma ye-plasma ingagcinwa esiqandisini ku-2-8 ℃ ngezinsuku eziyi-7 days kanye ne-cropressoal
I-3.all isampula igweme imijikelezo ye-freeze-thaw.

Inqubo yokufa
Uyacelwa ukuthi ufunde incwadi yokusebenza kwensimbi yokusebenza kwensimbi kanye nokufaka iphakheji ngaphambi kokuhlolwa.
1.Lay ubeka wonke ama-reagents namasampula wokushisa kwegumbi.
I-2.open them immune analyzer (Wiz-A101), faka ukungena ngemvume kwe-akhawunti ngokusho kwendlela yokusebenza yensimbi, bese ufaka isikhombimsebenzisi sokuthola.
I-3.Sc Ikhodi Yokucacisa Ukuqinisekisa Into Yokuhlola.
4.Thola ikhadi lokuhlola esikhwameni se-foil.
5.Shuma ikhadi lokuhlola ekhadini lekhadi, scan ikhodi ye-QR, bese unquma into yokuhlola.
I-5.Add 40μL serum noma isampula ye-plasma kwi-tampleent, futhi uhlanganise kahle ..
I-6.Add 80μL isixazululo isampula yesampula kahle yekhadi.
7.Chofoza inkinobho ethi "Ukuhlolwa okujwayelekile", ngemuva kwemizuzu engu-15, ithuluzi lizobona ngokuzenzakalelayo ikhadi lokuhlola, lingafunda imiphumela kusuka esikrinini sokubonisa sensimbi, bese uqopha / phrinta imiphumela yokuhlola.
8.Refer ekufundisweni kwe-immune Analyzer (Wiz-A101).

Amanani alindelwe
I-CK-MB <5ng / ml
Kunconywa ukuthi ilebhu ngalinye ngalinye lasungulwa uhla lwalo olujwayelekile olumele abantu balo.

Imiphumela yokuhlola nokutolika
.Imininingwane engenhla ingumphumela wokuhlolwa kwe-CK-MB reagent, futhi kuphakanyiswa ukuthi ilabhorethri ngalinye kufanele lisungule amanani anhlobo e-CK-MB alungele inani labantu kulesi sifunda. Imiphumela engenhla ingeyokubhekiswa kuphela.
.Imiphumela yale ndlela isebenza kuphela kumabanga okuthola amareferensi asungulwe kule ndlela, futhi akukho ukuqhathanisa okuqondile nezinye izindlela.
Izici ze-.

Ukugcinwa nokuqina
I-1.Ikhithi iyizinyanga eziyi-18 zeShelf-Life kusuka ngosuku lokukhiqiza. Gcina amakhithi angasetshenziswanga ngo-2-30 ° C. Ungabazi. Ungasebenzisi ngaphezu kosuku lokuphelelwa yisikhathi.
2.Ungakuvuli esikhwameni esivaliwe uze ulungele ukwenza isivivinyo, futhi ukuhlolwa okusetshenziswa okukodwa kuphakanyiswa ukuthi kusetshenziswe ngaphansi kwendawo edingekayo (lokushisa 2-35 ℃, umswakama we-40-90%) ngaphakathi kwamaminithi angama-60 ngokushesha ngangokunokwenzeka.
3.Sample Diloulent isetshenziswa ngokushesha ngemuva kokuvulwa.

Izexwayiso kanye nokuqapha
.Ikhithi kufanele ivalwe futhi ivikelwe kumswakama.
.Imininingwane emihle izoqinisekiswa ezinye izindlela.
.All amasampula azophathwa njengokungcoliswa okungaba khona.
.Ungasebenzisi i-reagent ephelelwe yisikhathi.
.Danga ukuguqula ama-reagents phakathi kwama-kits ane-lot ehlukile cha ..
.Ungasebenzisi kabusha amakhadi okuhlola nanoma yiziphi izesekeli ezilahlwayo.
.Kungani, isampula ngokweqile noma elincane lingaholela ekuphaphemeni komphumela.

LUkufanisa
.S nganoma yisiphi ama-antibodies asegulayo e-Assay asebenzisa, kungenzeka ukuthi kuphazanyiswe ngama-antibodies angomuntu alwa nobuntu (Hama) ku-specimen. Izinhlobo ezivela ezigulini ezithole amalungiselelo ama-antibodies e-monoclonal yokuxilongwa noma ukwelashwa kungaqukatha uHama. Izinhlobo ezinjalo zingadala imiphumela emibi noma engamanga.
Umphumela wokuhlola we-.Lokhuwo we-. .
.Lokhu i-realent isetshenziselwa kuphela ukuhlolwa kwe-serum ne-plasma. Kungase kungatholi umphumela onembile uma usetshenziselwa amanye amasampula anjenge-saliva nomchamo nokunye.

Izici Zokusebenza

REcabanga
1.Hansen JH, et al.hama ukuphazamiseka nge-murine monoclonal antibody-based immunody [j] .j we-Clin immunoassay, 1993,16: 294-299.
I-2.levinson ss.Imvelo yama-antibophilies ama-heterophilic kanye neqhaza ekuphazanyisweni kwe-immunoassay [j] .j we-mimunoassay, 1992,15: 108-114.

Ukhiye wezimpawu ezisetshenzisiwe:

	Kudivayisi yezokwelapha yokuxilongwa kwe-vitro
	Umenzi
	Gcina ngo-2-30 ℃
	Usuku lokuphelelwa isikhathi
	Ungaphinde usebenzise kabusha
	Isiqapheliso
	Bheka imiyalo yokusebenzisa

Xiamen Wiz Biotech Co., Ltd
Ikheli: 3-4 phansi, i-No.16 yokwakha, indawo yokusebenzela ye-bio-medical, 2030 wengjiao West Road, Haicang District, 3610266, xiamen, China
I-TELE: + 86-592-6808278
Ifeksi: + 86-592-6808279