Ikhithi yokuxilonga yeI-hypersensitive C-reactive protein

(ukuhlolwa kwe-fluorescence immunochromatographic)

Ukusetshenziswa kwe-in vitro diagnostic kuphela

Sicela ufunde le phakheji ngokucophelela ngaphambi kokuyisebenzisa futhi ulandele ngokuqinile imiyalelo. Ukuthembeka kwemiphumela yokuhlolwa akunakuqinisekiswa uma kukhona ukuchezuka emiyalweni ekule kokufakwa kwephakheji.

UKUSETSHENZISWA OKUHLOSIWE

I-Diagnostic Kit ye-hypersensitive C-reactive protein (fluorescence immunochromatographic assay) iwukuhlola kwe-fluorescence immunochromatographic ukuze kutholwe inani le-C-reactive protein (CRP) ku-serum yomuntu / plasma/ Igazi lonke. Kuyinkomba engaqondile yokuvuvukala. Wonke amasampula amahle kufanele aqinisekiswe ngezinye izindlela. Lokhu kuhlolwa kuhloselwe ukusetshenziswa ngochwepheshe bezempilo kuphela.

ISIFINYEZO

I-C-reactive protein iyiphrotheni yesigaba esibucayi ekhiqizwa ukugqugquzela i-lymphokine yesibindi namaseli e-epithelial. Itholakala ku-serum yomuntu, uketshezi lwe-cerebrospinal, uketshezi lwe-pleural nolwesisu, njll., futhi iyingxenye yendlela yokuzivikela engaqondile. 6-8h ngemva kokuvela kokutheleleka ngebhaktheriya, i-CRP yaqala ukwanda, 24-48h yafinyelela inani eliphakeme, futhi inani eliphakeme lingafinyelela izikhathi ezingamakhulu ezivamile. Ngemva kokuqedwa kokutheleleka, i-CRP yehla kakhulu futhi yabuyela kwesijwayelekile phakathi nesonto elilodwa. Kodwa-ke, i-CRP ayinyuki kakhulu esimweni sokutheleleka ngegciwane, okunikeza isisekelo sokuhlonza izinhlobo zezifo ezithathelwanayo kusenesikhathi, futhi iyithuluzi lokuhlonza izifo ezibangelwa amagciwane noma amagciwane.

ISIMISO SENKQUBO

Ulwelwesi lwedivayisi yokuhlola lumbozwe ngamasosha omzimba e-CRP endaweni yokuhlola kanye ne-anti-rabbit IgG yamasosha omzimba endaweni yokulawula. Iphedi elilebula limbozwa nge-fluorescence ebhalwe ukuthi anti-CRP antibody kanye ne-rabbit IgG kusengaphambili. Lapho kuhlolwa isampula elihle, i-antigen ye-CRP kusampula ihlangana ne-fluorescence ebhalwe ukuthi i-anti-CRP antibody, bese yenza ingxube yamasosha omzimba. Ngaphansi kwesenzo se-immunochromatography, ukugeleza okuyinkimbinkimbi kubheke ephepheni elimuncayo, lapho inkimbinkimbi idlula isifunda sokuhlola, ihlanganiswe ne-anti-CRP yokuhlanganisa i-antibody, yakha inkimbinkimbi entsha. Izinga le-CRP lihlotshaniswa kahle nesignali ye-fluorescence, futhi ukugxila kwe-CRP kusampula kungatholwa nge-fluorescence immunoassay assay.