I-Diagnostic Kit ye-Estradiol(Fleorescence Immunochrographic Assay)
Ngokusetshenziswa kokuxilongwa kwe-vitro kuphela
Uyacelwa ukuthi ufunde leli phakethe Faka ngokucophelela ngaphambi kokusebenzisa futhi ulandele imiyalo ngokuqinile. Ukwethembeka kwemiphumela ye-Assay akunakuqinisekiswa uma kukhona ukuphambuka emiyalweni ekuleW kufakiwe.

Ukusetshenziswa okuhlosiwe
I-Diagnostic Kit ye-Estradiol (Fleorescence Immunochrographic Assay is a Fleorescence Immunochrogragraphic assay for the aughtative detection deten detection of estradiol (e2) ku-auxitisis serum noma i-plasma, okusetshenziselwa ukuxilongwa okuyisizayo reagent.Isampula enhle kumele kuqinisekiswe ezinye izindlela. Lokhu kuhlola kuhloselwe ukusetshenziswa kwezempilo kuphela.

Ukubeka kafushane
I-Estradiol (E2) iyi-hormone ebaluleke kakhulu futhi esebenza kakhulu e-estrogen.its molecular isisindo ngu-272.3 D.in General, kwabesifazane abakhulelwe, amaseli we-lotueal kanye namaseli we-luteal ngesikhathi sokukhulelwa kwe-follicular. I-E2 ithetshulwa ikakhulukazi yi-placenta, kanti amadoda akhiqizwa ikakhulukazi ama-testicles.After afaka igazi, u-1% kuya ku-3% angabophisi amaprotheni, ama-40%), nabanye babopha I-Albemin, i-Metabolize evela esibindini ngama-sulfates ancibilikiswe amanzi noma ama-gluconaldehyde esters, futhi akhishwe kwi-Urine.e2 iyinkomba ebalulekile ye-hormone yokuhlola umsebenzi we-ovari.

Umgomo wenqubo
Ulwelwesi lwedivayisi yokuhlola luhlanganiswe ne-conjugate ye-BSA kanye ne-estradiol esifundeni sokuhlola kanye nembuzi anti Unogwaja I-IGG antibody on the control. I-Marker Pad ihlanganiswe ngu-Fluorescence Mark anti E2 antibody kanye noRabbit IGG kusengaphambili. Lapho uhlola isampula, i-E2 ngesampula ihlangana ne-fluorescence emakwe anti E2 antibody, futhi akha inhlanganisela yokuzivikela. Ngaphansi kwesenzo se-Immunochrotography, ukugeleza okuyinkimbinkimbi kokuqondisa kwephepha lokungena, lapho i-Fluorescent Marker izohlanganiswa ne-estradiol kwi-membradiol ku-askradiol ukuxhumana okungalungile kwesiginali ye-fluorescence, kanye Ukuhlushwa kwe-estradiol ngesampula kungatholwa yi-fluorence imunoassay assay.

Ama-reagents nezinto zokwenziwa

25T Iphakethe Izakhi:
.Test Card ngakunye i-foil ipaniwe nge-desiccant 25t
.A Isixazululo 25t
.B Isixazululo 1
.Fackage Faka 1

Izinto ezidingekayo kodwa azinikeziwe
Isithakongo esiqoqiwe sesampula, isibali sikhathi

Ukuqoqwa kwesampula nesitoreji
1.Izimpumu ezihlolwe zingaba yi-serum, i-heparin anticoaagulant plasma noma i-edta anticoaagulant plasma.
2.Faka kumasu ajwayelekile ukuqoqa isampula. Isampula ye-serum noma ye-plasma ingagcinwa esiqandisini ku-2-8 ℃ ngezinsuku eziyi-7 days kanye ne-cropressoal
.Aka amasampula agweme imijikelezo ye-freeze-thaw.

Inqubo yokufa
Inqubo yokuhlola ithuluzi ibona incwajana ye-immunoanalyzer. Inqubo yokuhlola i-Reagent imi kanjena
1.Lay ubeka wonke ama-reagents namasampula wokushisa kwegumbi.
I-2.open them immune analyzer (Wiz-A101), faka ukungena ngemvume kwe-akhawunti ngokusho kwendlela yokusebenza yensimbi, bese ufaka isikhombimsebenzisi sokuthola.
I-3.Sc Ikhodi Yokucacisa Ukuqinisekisa Into Yokuhlola.
3.Thola ikhadi lokuhlola esikhwameni se-foil.
I-4.insert ikhadi lokuhlola elisekhadini lekhadi, scan ikhodi ye-QR, bese unquma into yokuhlola.
I-5.Add 30μL serum noma isampula ye-plasma kwikhambi, uhlanganise kahle.
6.ADD 20μL B Isixazululo sengxube engenhla, uhlanganise kahle.
Shiya ingxube ye20imizuzu.
Faka ingxube ye-80μL eyisampula kahle ekhadini.
Chofoza inkinobho ethi "Ukuhlolwa okujwayelekile", ngemuva kwemizuzu eyi-10, ithuluzi lizobona ngokuzenzakalelayo ikhadi lokuhlola, lingafunda imiphumela kusuka esikrinini sokubonisa sensimbi, bese uqopha / phrinta imiphumela yokuhlola.
Bheka imiyalo ye-Immune Analyzer ephathekayo (Wiz-A101).

Imiphumela yokuhlola nokutolika

.Imininingwane engenhla iyisikhashana esisungulwe idatha yokutholwa kwale kit, futhi kuphakanyiswa ukuthi ilabhorethri ngalinye kufanele lisungule isikhawu sokubaluleka komtholampilo.
.I-straction ye-estradiol iphakeme kunebanga lereferensi, futhi izinguquko zomzimba noma impendulo yokucindezela kufanele ikhishwe ngaphandle kwe-
.Imiphumela yale ndlela isebenza kuphela ebangeni lereferensi elisungulwe yile ndlela, futhi imiphumela ayiqhathaniswa ngqo nezinye izindlela.
Izici ze-.

Ukugcinwa nokuqina
I-1.Ikhithi iyizinyanga eziyi-18 zeShelf-Life kusuka ngosuku lokukhiqiza. Gcina amakhithi angasetshenziswanga ngo-2-30 ° C. Ungabazi. Ungasebenzisi ngaphezu kosuku lokuphelelwa yisikhathi.
2.Ungakuvuli esikhwameni esivaliwe uze ulungele ukwenza isivivinyo, futhi ukuhlolwa okusetshenziswa okukodwa kuphakanyiswa ukuthi kusetshenziswe ngaphansi kwendawo edingekayo (lokushisa 2-35 ℃, umswakama we-40-90%) ngaphakathi kwamaminithi angama-60 ngokushesha ngangokunokwenzeka.
3.Sample Diloulent isetshenziswa ngokushesha ngemuva kokuvulwa.

Izexwayiso kanye nokuqapha
.Ikhithi kufanele ivalwe futhi ivikelwe kumswakama.
.Imininingwane emihle izoqinisekiswa ezinye izindlela.
.All amasampula azophathwa njengokungcoliswa okungaba khona.
.Ungasebenzisi i-reagent ephelelwe yisikhathi.
.Danga ukuguqula ama-reagents phakathi kwama-kits ane-lot ehlukile cha ..
.Ungasebenzisi kabusha amakhadi okuhlola nanoma yiziphi izesekeli ezilahlwayo.
.Kungani, isampula ngokweqile noma elincane lingaholela ekuphaphemeni komphumela.

LUkufanisa
.S nganoma yisiphi ama-antibodies asegulayo e-Assay asebenzisa, kungenzeka ukuthi kuphazanyiswe ngama-antibodies angomuntu alwa nobuntu (Hama) ku-specimen. Izinhlobo ezivela ezigulini ezithole amalungiselelo ama-antibodies e-monoclonal yokuxilongwa noma ukwelashwa kungaqukatha uHama. Izinhlobo ezinjalo zingadala imiphumela emibi noma engamanga.
Umphumela wokuhlola we-.Lokhuwo wethenjwa kuphela, akufanele ukhonze njengeso sisekelo sokuxilongwa kokwelashwa nokwelashwa, ukuphathwa kwemitholampilo yeziguli kufanele kube ukucatshangelwa okuphelele kuhlanganiswe nezimpawu zalo,
.Umlando we-Lagerolarical, okunye ukuhlolwa kwelebhu, impendulo yokwelashwa, i-epidemiology kanye neminye imininingwane.
.Lokhu i-realent isetshenziselwa kuphela ukuhlolwa kwe-serum ne-plasma. Kungase kungatholi umphumela onembile uma usetshenziselwa amanye amasampula anjenge-saliva nomchamo nokunye.
Izici Zokusebenza

REcabanga
1.Hansen JH, et al.hama ukuphazamiseka nge-murine monoclonal antibody-based immunody [j] .j we-Clin immunoassay, 1993,16: 294-299.
I-2.levinson ss.Imvelo yama-antibophilies ama-heterophilic kanye neqhaza ekuphazanyisweni kwe-immunoassay [j] .j we-mimunoassay, 1992,15: 108-114.

Ukhiye wezimpawu ezisetshenzisiwe:

	Kudivayisi yezokwelapha yokuxilongwa kwe-vitro
	Umenzi
	Gcina ngo-2-30 ℃
	Usuku lokuphelelwa isikhathi
	Ungaphinde usebenzise kabusha
	Isiqapheliso
	Bheka imiyalo yokusebenzisa

Xiamen Wiz Biotech Co., Ltd
Ikheli: 3-4 phansi, i-No.16 yokwakha, indawo yokusebenzela ye-bio-medical, 2030 wengjiao West Road, Haicang District, 3610266, xiamen, China
I-TELE: + 86-592-6808278
Ifeksi: + 86-592-6808279