Ikhithi yokuxilonga yeCardiac Troponin I Myoglobin kanye ne-Isoenzyme MB ye-Creatine Kinase
Ikhithi yokuxilonga yeCardiac Troponin I ∕Isoenzyme MB ye-Creatine Kinase ∕Myoglobin
Indlela yokusebenza:Fluorescence Immunochromatographic Assay
Ulwazi lokukhiqiza
| Inombolo Yemodeli | cTnI/CK-MB/MYO | Ukupakisha | 25 Ukuhlolwa/ikhithi, 30kits/CTN |
| Igama | Ikhithi yokuxilonga yeCardiac Troponin I ∕Isoenzyme MB ye-Creatine Kinase ∕Myoglobin | Ukuhlukaniswa kwezinsimbi | Ikilasi II |
| Izici | Ukuzwela okuphezulu, ukusebenza okulula | Isitifiketi | CE/ ISO13485 |
| Ukunemba | > 99% | Impilo yeshelufu | Iminyaka Emibili |
| Indlela yokwenza | I-Fluorescence Immunochromatographic Assay | Isevisi ye-OEM/ODM | Iyatholakala |
UKUSETSHENZISWA OKUHLOSIWE
Le kit iyasebenza ekutholweni kwe-in vitro quantitative kokugxila kwezimpawu zokulimala kwe-myocardial yenhliziyo.
i-troponin I, isoenzyme MB ye-creatine kinasein ne-myoglobin ku-serum yomuntu/i-plasma/isampula yegazi lonke, kanye
ilungele ukuxilongwa kwe-myocardial infarction. Le kit inikeza kuphela imiphumela yokuhlolwa kwe-cardiac troponin I,
I-isoenzyme MB ye-creatine kinasein ne-myoglobin, futhi imiphumela etholiwe izosetshenziswa ngokuhlangana neminye
ulwazi lomtholampilo ukuze luhlaziywe. Kufanele isetshenziswe kuphela ochwepheshe bezempilo.
Inqubo yokuhlola
| 1 | Ngaphambi kokusebenzisa i-reagent, funda iphakheji ngokucophelela futhi uzijwayeze nezinqubo zokusebenza. |
| 2 | Khetha imodi yokuhlola evamile ye-WIZ-A101 ephathekayo yomzimba yokuhlaziya |
| 3 | Vula iphakheji yesikhwama se-aluminium foil ye-reagent bese ukhipha idivayisi yokuhlola. |
| 4 | Faka ngokuvundlile idivayisi yokuhlola ku-slot ye-immune analyzer. |
| 5 | Ekhasini lasekhaya le-interface yokusebenza ye-immune analyzer, chofoza okuthi "Standard" ukuze ufake isixhumi esibonakalayo sokuhlola. |
| 6 | Chofoza okuthi “QC Scan” ukuze uskene ikhodi ye-QR ohlangothini lwangaphakathi lwekhithi; amapharamitha ahlobene nekhithi ensimbini bese ukhetha uhlobo lwesampula. Qaphela: Inombolo ngayinye yeqoqo lekhithi izoskenwa ngesikhathi esisodwa. Uma inombolo ye-batch iskeniwe, bese weqa lesi sinyathelo. |
| 7 | Hlola ukuvumelana "Kwegama Lomkhiqizo", "Inombolo Yeqoqo" njll. kusixhumi esibonakalayo sokuhlola ngolwazi olukulebula yekhithi. |
| 8 | Khipha isampula encibilikisiwe olwazini olungaguquki, engeza u-80μL we-serum/plasma/isampula legazi lonke, bese uwaxuba kahle; |
| 9 | Engeza isixazululo esixutshwe kahle esingu-80µL esishiwo ngenhla emthonjeni wedivayisi yokuhlola; |
| 10 | Ngemva kokufakwa okuphelele kwesampula, chofoza "Isikhathi" futhi isikhathi sokuhlola esisele sizovezwa ngokuzenzakalelayo kusixhumi esibonakalayo. |
| 11 | I-immune analyzer izoqedela ukuhlola nokuhlaziya ngokuzenzakalelayo lapho isikhathi sokuhlolwa sesifinyelelwe. |
| 12 | Ngemuva kokuthi ukuhlolwa kwe-immune analyzer sekuqediwe, umphumela wokuhlolwa uzovezwa kusixhumi esibonakalayo sokuhlola noma ungabukwa “Ngomlando” ekhasini lasekhaya lesixhumi esibonakalayo sokusebenza. |
Qaphela: isampula ngayinye izofakwa ngepayipi ngepayipi elahlwayo elihlanzekile ukuze kugwenywe ukungcoliswa okuphambanayo.
Ukuphakama
Isikhathi sokuhlola: 10-15mins
Isitoreji:2-30℃/36-86℉
Indlela yokusebenza:Fluorescence Immunochromatographic Assay
Isici:
• Ukuzwela okuphezulu
• umphumela wokufunda emizuzwini eyi-15
• Ukusebenza okulula
• Ukuhlola okungu-3 ngesikhathi esisodwa, ukonga izikhathi.
• Ukunemba okuphezulu
Ukusebenza Kwemtholampilo
Ukusebenza komtholampilo kwalo mkhiqizo kuhlolwa ngokuqoqwa kwezimo ezingu-150 zamasampula omtholampilo.
a) Esimeni sento ye-cTnI, ikhithi ehambelanayo emakethe ye-chemiluminescence assay isetshenziswa njengereferensi yesithenjwa,
imiphumela yokutholwa iqhathaniswe futhi ukuqhathaniswa kwayo kuhlolisiswe ngokuhlehla komugqa, futhi
ama-coefficients wokuhlanganisa wezilinganiso ezimbili ngu-Y=0.975X+0.074 kanye no-R=0.9854 ngokulandelanayo;
b) Esimeni sento ye-CK-MB, ikhithi ehambelanayo emakethe ye-electrochemiluminescence assays isetshenziswa njengereferensi
i-reagent, imiphumela yokuthola iqhathaniswe futhi ukuqhathanisa kwayo kuhlolisiswe ngomugqa
ukuhlehla, nama-coefficients wokuhlobanisa wezilinganiso ezimbili ngu-Y=0.915X+0.242 kanye no-R=0.9885 ngokulandelanayo.
c) Uma kwenzeka into ye-MYO, ikhithi ehambelanayo emakethe ye-fluor immunoassays exazululwe isikhathi esetshenziswa njengereferensi
i-reagent, imiphumela yokuthola iqhathaniswe futhi ukuqhathanisa kwayo kuhlolisiswe ngomugqa
ukuhlehla, nama-coefficients ahlotshaniswayo wezilinganiso ezimbili ngu-y=0.989x+2.759 kanye no-R=0.9897 ngokulandelanayo.
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