Ikhithi yokuxilonga yeI-Calprotectin(I-Fluorescence Immunochromatographic Assay)
Ukusetshenziswa kwe-in vitro diagnostic kuphela
Sicela ufunde le phakheji ngokucophelela ngaphambi kokuyisebenzisa futhi ulandele ngokuqinile imiyalelo. Ukuthembeka kwemiphumela yokuhlolwa akunakuqinisekiswa uma kukhona ukuchezuka emiyalweni ekule kokufakwa kwephakheji.

UKUSETSHENZISWA OKUHLOSIWE
Ikhithi yokuxilonga yeI-Calprotectin(I-Cal) ifanele ukunqunywa komthamo wendle yomuntu I-Cal by fluorescence immunochromatographic assay, enesesekeli esibalulekile sokuxilonga isifo samathumbu avuvukalayo. Wonke amasampula amahle kufanele aqinisekiswe ngezinye izindlela. Lokhu kuhlolwa kuhloselwe ukusetshenziswa ngochwepheshe bezempilo kuphela.

ISIFINYEZO
I-Cal iyi-heterodimer, eyakhiwa i-MRP 8 ne-MRP 14^[1]. Itholakala ku-neutrophils cytoplasm futhi iboniswa kuma-mononuclear cell membranes. I-Cal iyi-acute phase proteins, inesigaba esizinzile cishe isonto elilodwa endle yomuntu, kunqunywa ukuthi ibe umaka wezifo ezivuvukalayo.^[2-3]. Ikhithi iwukuhlola komthamo olula, okubukwayo okuthola i-Cal endle yomuntu, inokuzwela okuphezulu nokubonakala okuqinile. Ukuhlolwa okusekelwe ekucacisweni okuphezulu kwesimiso sokuphendula isangweji sama-antibodies aphindwe kabili kanye nobuchwepheshe bokuhlaziya i-fluorescence immunochromatographic assay, okunganikeza umphumela phakathi nemizuzu eyi-15.

ISIMISO SENKQUBO
Umucu une-anti-Cal coating antibody endaweni yokuhlola, eboshelwe ku-membrane chromatography kusengaphambili. Iphedi elebula limbozwe i-fluorescence ebhalwe ukuthi i-anti-Cal antibody kusengaphambili. Lapho kuhlolwa isampula elihle, isampula ye-Cal ingaxutshwa ne-fluorescence ebhalwe ukuthi i-anti-Cal antibody, futhi yakhe inhlanganisela yokuzivikela komzimba. Njengoba ingxube ivunyelwa ukuthi ifuduke eduze komugqa wokuhlola, inkimbinkimbi ye-Cal conjugate ithathwa yi-anti-Cal coating antibody kulwelwesi futhi yakha inkimbinkimbi. Amandla e-fluorescence ahlotshaniswa kahle nokuqukethwe kwe-Cal. I-Cal esesampula ingatholwa nge-fluorescence immunoassay analyzer.

AMA-REAGENTS KANYE NEZINTO EZINIKEZELWAYO

25T izingxenye zephakheji:
Ikhadi lokuhlola i-foil ngayinye efakwe esikhwameni se-desiccant 25T
Isampula ye-diluent 25T
Faka iphakheji 1

IZINTO EZIDINGEKAYO KODWA AKUNIKEZWE
Isitsha seqoqo lesampula, isibali sikhathi

UKUQOQWA KANYE NESIGCINISO ESAMPULA
1.Sebenzisa isitsha esihlanzekile esilahlwayo ukuqoqa isampula yendle entsha, bese ihlolwa ngokushesha. Uma ingeke ihlolwe ngokushesha, sicela igcinwe ku-2-8°C izinsuku ezi-3 noma ngaphansi kuka-15°C izinyanga ezingu-6.
2.Khipha udondolo lwesampula, ulifake esampuleni yobulongwe, phinda isenzo izikhathi ezi-3, thatha izingxenye ezehlukene zesampula yobulongwe isikhathi ngasinye, bese ubuyisela induku yesampula, bopha qini bese uxhawula kahle, Noma usebenzisa isampula yesampula ekhethiwe. cishe isampula lobulongwe elingu-50mg, bese lifaka ishubhu lesampula lobulongwe eliqukethe isampula lokuhlanjululwa, bese liyisikulufa ngokuqinile.
3.Sebenzisa amasampula e-pipette alahlwayo thatha isampula yendle esigulini sohudo, bese wengeza amaconsi ama-3 (cishe u-100µL) eshubhuni lokusampula le-fecal bese unyakazisa kahle.

Amanothi:
1.Gwema imijikelezo yokuqhwaza.
2.Thaw amasampula ekamelweni lokushisa ngaphambi kokusetshenziswa.

INQUBO YOKUHLOLA

Sicela ufunde imanuwali yokusebenza kwezinsimbi kanye nokufakwa kwephakheji ngaphambi kokuhlola.
1.Beka eceleni wonke ama-reagents namasampuli ekamelweni lokushisa.
2.Vula i-Portable Immune Analyzer(WIZ-A101), faka ukungena ngemvume kwephasiwedi ye-akhawunti ngokwendlela yokusebenza yethuluzi, bese ufaka isixhumi esibonakalayo sokubona.
3.Skena ikhodi yokuhlonza ukuze uqinisekise into yokuhlola.
4.Khipha ikhadi lokuhlola esikhwameni se-foil.
5.Faka ikhadi lokuhlola endaweni yekhadi, skena ikhodi ye-QR, bese unquma into yokuhlola.
6.Khipha ikepisi kushubhu yesampula bese ulahla amathonsi amabili okuqala esampula ehlanjululiwe, engeza amaconsi ama-3 (cishe u-100uL) alikho ibhamuza elihlanjululwe isampula lime mpo futhi kancane ungene emthonjeni wesampula wekhadi eline-dispette enikeziwe.
7.Chofoza inkinobho ethi "standard test", ngemva kwemizuzu engu-15, ithuluzi lizothola ngokuzenzakalelayo ikhadi lokuhlola, lingakwazi ukufunda imiphumela esikrinini sokubonisa sensimbi, futhi lirekhode / liphrinte imiphumela yokuhlolwa.
8.Bheka umyalelo we-Portable Immune Analyzer(WIZ-A101).

IZIMALI EZILINDELEKILE
Ikhalori <60μg/g
Kutuswa ukuthi ilabhorethri ngayinye isungule ububanzi bayo obujwayelekile obumelela isiguli sayo.

IMIPHUMELA YOKUHLOLA NOKUTOLIZWA
1.I-Cal kusampula ingaphezu kuka-60μg/g, futhi kufanele ikhiphe uguquko lwesimo somzimba. Imiphumela ayijwayelekile ngempela futhi kufanele kutholakale ukuthi inezimpawu zomtholampilo.
2.Imiphumela yale ndlela isebenza kuphela kububanzi bereferensi obusungulwe kule ndlela, futhi akukho ukuqhathanisa okuqondile nezinye izindlela.
3.Ezinye izici zingabangela amaphutha emiphumeleni yokuthola, okuhlanganisa izizathu zobuchwepheshe, amaphutha okusebenza nezinye izici zesampula.

UKUGCINWA NOKUZInza
1.Ikhithi iyimpilo yeshelufu yezinyanga eziyi-18 kusukela ngosuku lokukhiqizwa kwayo. Gcina amakhithi angasetshenzisiwe ku-2-30°C. UNGAQISHI. Ungasebenzisi ngale kosuku lokuphelelwa yisikhathi.
2.Ungasivuli isikhwama esivaliwe kuze kube yilapho usulungele ukwenza isivivinyo, futhi ukuhlolwa okukodwa kuphakanyiswa ukuthi kusetshenziswe ngaphansi kwendawo edingekayo (izinga lokushisa elingu-2-35℃, umswakama 40-90%) phakathi kwemizuzu engama-60 ngokushesha. ngangokunokwenzeka.
I-3.I-diluent yesampula isetshenziswa ngokushesha ngemva kokuvulwa.

IZIXWAYISO NEZIQINISEKISO
.Ikhithi kufanele ivalwe futhi ivikelwe emswakameni.
.Zonke izibonelo ezinhle zizoqinisekiswa ngezinye izindlela.
.Zonke izifanekiso zizophathwa njengamandla angcolisayo.
.UNGAsebenzisi i-reagent ephelelwe yisikhathi.
.UNGAshintshisani ama-reagents phakathi kwamakhithi anendawo ehlukile No..
.UNGAphinde usebenzise amakhadi okuhlola nanoma yiziphi izisekeli ezilahlwayo.
.Ukungasebenzi kahle, isampula eliningi noma elincane lingaholela ekuphambukeni kwemiphumela.

UMKHAWULO
.Njenganoma yikuphi ukulinganisa okusebenzisa amasosha omzimba egundane, kungenzeka ukuthi kuphazamiseke amasosha omzimba alwa namagundane (HAMA) esibonelweni. Izibonelo zeziguli ezithole amalungiselelo e-monoclonal antibodies ukuze zixilongwe noma zelashwe zingaqukatha i-HAMA. Izibonelo ezinjalo zingadala imiphumela engemihle noma engemihle engamanga.
.Lo mphumela wokuhlolwa ngowenkomba yomtholampilo kuphela, akufanele usebenze njengesisekelo sokuxilongwa nokwelashwa kweziguli, ukuphathwa komtholampilo kweziguli kufanele kucatshangelwe ngokugcwele kuhlanganiswe nezimpawu zayo, umlando wezokwelapha, okunye ukuhlolwa kwaselabhorethri, impendulo yokwelashwa, i-epidemiology kanye nolunye ulwazi. .
.Lesi sisebenzi sisetshenziselwa ukuhlola indle kuphela. Ingase ingatholi umphumela onembile uma isetshenziselwa amanye amasampula afana namathe nomchamo nokunye.

IZIMPAWU ZOKUSEBENZA

IZIKHOMBISI
1.Li,G.&Y.L.Li.Ubudlelwano phakathi kwe-calcium nesifo somtholampilo[J].The Journal of Practical Medicine,2007,23(15)
2.Han,W.,Xu,JM,et al.Ucwaningo lomtholampilo lwe-calamine ne-lactoferrin kuziguli ezine-ulcerative colitis[J].I-Chinese Journal of Gastroenterology,2010,15(4)
3.Wang,ZH,Guo,HB,et al.Ucwaningo mayelana nobudlelwano phakathi kwe-fecal calamine nesifo samathumbu esivuvukalayo[J]. Isayensi Yezobuchwepheshe Nobunjiniyela, 2010-03,10(8)

Ukhiye wezimpawu ezisetshenzisiwe:

	In Vitro Diagnostic Medical Device
	Umkhiqizi
	Gcina ku-2-30 ℃
	Usuku lokuphelelwa isikhathi
	Ungaphinde Usebenzise
	ISEXWAYISO
	Bheka Imiyalo Yokusetshenziswa

I-Xiamen Wiz Biotech CO., LTD
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