I-Diagnostic Kit yeI-Calprotectin(Fleorescence Immunochrographic Assay)
Ngokusetshenziswa kokuxilongwa kwe-vitro kuphela
Uyacelwa ukuthi ufunde leli phakethe Faka ngokucophelela ngaphambi kokusebenzisa futhi ulandele imiyalo ngokuqinile. Ukwethembeka kwemiphumela ye-Assay akunakuqinisekiswa uma kukhona ukuphambuka emiyalweni ekuleW kufakiwe.

Ukusetshenziswa okuhlosiwe
I-Diagnostic Kit yeCalprotectin (Cal) ilungele ukunqunywa kwenani eliningi le-Human Faeces Cal yi-Fluorescesrochrochrographigraphigraph Assay yesifo sokuvuvukala se-ukuvuvukala.Isampula ebonakalayo kufanele iqinisekiswe ezinye izindlela. Lokhu kuhlola kuhloselwe ukusetshenziswa kwezempilo kuphela.

Ukubeka kafushane
I-CAL iyi-heterodimer, eyakhiwa i-MRP 8 ne-MRP 14^[1]. Ikhona kuma-neutrophils cytoplasm futhi avezwe ngolwelwesi lwamaseli e-mononuclear. I-CAL ingamaprotheni esigaba esibi, inesigaba esizinzile esiseduze kwesonto elilodwa kuma-faces abantu, izimisele ukuba ngumaka wezifo zamathumbu zamathumbu^[2-3]. I-kit iyisivivinyo esilula, esibonakalayo esibona i-cal emalondolo antwini, inokuzwela okuphezulu nokucaciswa okuqinile. Ukuhlolwa kususelwa ekucacisweni okuphezulu kwama-sandwich reaction antibodies antibodies kanye ne-fluorescence immunochrographic assay assay assay ukuhlaziya ama-assay assay ukuhlaziya ama-assay assay ukuhlaziya ama-assay assay ukuhlaziya ubuchwepheshe, okunganikeza umphumela kungakapheli imizuzu eyi-15.

Umgomo wenqubo
I-strip ine-anti-cal coating antilod on Test Region, efakwe ku-chromatography kusengaphambili. I-Lable Pad ihlanganiswe yi-fluorescence ilebula antibody ngaphambi kwesikhathi. Lapho uvivinya isampula elimuhle, i-CAL ngesampula ingahlanganiswa ne-fluorescence ilebula antibody antibody, bese wenza umxube wokuzivikela. Njengoba ingxube ivunyelwe ukufuduka eceleni kwe-test strip, iCal Conjugate Complex ithathwe yi-anti-cal coating coating coing antibody ku-membrane namafomu. Ukuqina kwe-fluorescence kuhlotshaniswa kahle nokuqukethwe kwekhwala. I-CAL ngesampula ingatholwa yi-Fluorence Immunoassay Analyzer.

Ama-reagents nezinto zokwenziwa

25T Iphakethe Izakhi:
Ikhadi lokuhlola ngawodwana ahlelwe nge-desiccant 25t
Isampula dilients 25t
Faka iphakheji 1

Izinto ezidingekayo kodwa azinikeziwe
Isithakongo esiqoqiwe sesampula, isibali sikhathi

Ukuqoqwa kwesampula nesitoreji
1.Sebenzisa isitsha esihlanzekile esilahlwayo sokuqoqa isampula esisha se-Faeces, futhi sahlolelwa ngokushesha. Uma kungahloliswa ngokushesha, sicela ugcinwe ku-2-8 ° C izinsuku ezintathu noma ngaphansi -15 ° C izinyanga eziyisithupha.
2.Thumela induku yesampula, ifakwe kwisampula ye-Faeces Times, thatha izingxenye ezihlukile zesampula ye-Faeces isikhathi ngasinye, bese ubeka i-Sampling Stick emuva, noma unikine kahle, noma usebenzise i-Sampling Stick Cishe isampula ye-50mg Faeces, bese ufaka i-Faeces Samps Tube equkethe i-dilution yesampula, bese isikulufa ngokuqinile.
3.Sebenzisa isampula ye-Pipette elahlekile thatha isampula le-Faeces kusuka esigulini sohudo, bese wengeza amaconsi ama-3 (cishe ama-100μL) kwishubhu lesampula le-fecal bese linyakazisa kahle.

Amanothi:
I-1.Avoid Freeze-Thaw Cycles.
Amasampula we-2.thawtha egumbini lokushisa lekamelo ngaphambi kokusetshenziswa.

Inqubo yokufa

Uyacelwa ukuthi ufunde incwadi yokusebenza kwensimbi yokusebenza kwensimbi kanye nokufaka iphakheji ngaphambi kokuhlolwa.
1.Lay ubeka wonke ama-reagents namasampula wokushisa kwegumbi.
I-2.open them immune analyzer (Wiz-A101), faka ukungena ngemvume kwe-akhawunti ngokusho kwendlela yokusebenza yensimbi, bese ufaka isikhombimsebenzisi sokuthola.
I-3.Sc Ikhodi Yokucacisa Ukuqinisekisa Into Yokuhlola.
4.Thola ikhadi lokuhlola esikhwameni se-foil.
5.Shuma ikhadi lokuhlola ekhadini lekhadi, scan ikhodi ye-QR, bese unquma into yokuhlola.
6.Remove cap kusuka kushubhu eyisampula futhi ulahle amasampula amabili okuqala ahlanjululwe, engeza amaconsi ama-3 (cishe u-100Ul) Ayikho i-Bubble evuselelwe i-verticaly futhi kancane kancane ekhadini elinikezwe ngekhadi elinikeziwe.
7.Chofoza inkinobho ethi "Ukuhlolwa okujwayelekile", ngemuva kwemizuzu engu-15, ithuluzi lizobona ngokuzenzakalelayo ikhadi lokuhlola, lingafunda imiphumela kusuka esikrinini sokubonisa sensimbi, bese uqopha / phrinta imiphumela yokuhlola.
8.Refer ekufundisweni kwe-immune Analyzer (Wiz-A101).

Amanani alindelwe
Cal <60μg / g
Kunconywa ukuthi ilebhu ngalinye ngalinye lasungulwa uhla lwalo olujwayelekile olumele abantu balo.

Imiphumela yokuhlola nokutolika
I-1.I-CAL ngesampula ingaphezulu ka-60μ / g, futhi kufanele ilawule ukuguqulwa kwesimo somzimba. Imiphumela ayijwayelekile futhi kufanele kutholakale ukuthi inezimpawu zomtholampilo.
2.Imiphumela yale ndlela isebenza kuphela kumabanga okuthola amareferensi asungulwe kule ndlela, futhi akukho ukuqhathanisa okuqondile nezinye izindlela.
Izici eziqinisekisiwe ze-3.Other nazo zingadala amaphutha emiphumeleni yokuthola, kufaka phakathi izizathu zobuchwepheshe, amaphutha okusebenza nezinye izinto zesampula.

Ukugcinwa nokuqina
I-1.Ikhithi iyizinyanga eziyi-18 zeShelf-Life kusuka ngosuku lokukhiqiza. Gcina amakhithi angasetshenziswanga ngo-2-30 ° C. Ungabazi. Ungasebenzisi ngaphezu kosuku lokuphelelwa yisikhathi.
2.Ungakuvuli esikhwameni esivaliwe uze ulungele ukwenza isivivinyo, futhi ukuhlolwa okusetshenziswa okukodwa kuphakanyiswa ukuthi kusetshenziswe ngaphansi kwendawo edingekayo (lokushisa 2-35 ℃, umswakama we-40-90%) ngaphakathi kwamaminithi angama-60 ngokushesha ngangokunokwenzeka.
3.Sample Diloulent isetshenziswa ngokushesha ngemuva kokuvulwa.

Izexwayiso kanye nokuqapha
.Ikhithi kufanele ivalwe futhi ivikelwe kumswakama.
.Imininingwane emihle izoqinisekiswa ezinye izindlela.
.All amasampula azophathwa njengokungcoliswa okungaba khona.
.Ungasebenzisi i-reagent ephelelwe yisikhathi.
.Danga ukuguqula ama-reagents phakathi kwama-kits ane-lot ehlukile cha ..
.Ungasebenzisi kabusha amakhadi okuhlola nanoma yiziphi izesekeli ezilahlwayo.
.Kungani, isampula ngokweqile noma elincane lingaholela ekuphaphemeni komphumela.

Ukukhawulelwa
.S nganoma yisiphi ama-antibodies asegulayo e-Assay asebenzisa, kungenzeka ukuthi kuphazanyiswe ngama-antibodies angomuntu alwa nobuntu (Hama) ku-specimen. Izinhlobo ezivela ezigulini ezithole amalungiselelo ama-antibodies e-monoclonal yokuxilongwa noma ukwelashwa kungaqukatha uHama. Izinhlobo ezinjalo zingadala imiphumela emibi noma engamanga.
Umphumela wokuhlola we-.Lokhuwo we-. .
.Lokhu i-realent isetshenziselwa kuphela ukuhlolwa kwe-fecal. Kungase kungatholi umphumela onembile uma usetshenziselwa amanye amasampula anjenge-saliva nomchamo nokunye.

Izici Zokusebenza

Ukunqubekela phambili
1.li, g. & Y.l.li.Li.Umfolo [j] .Iphepha lezokwelapha ezisebenzayo, 2007,23 (15)
2.Han, W.
3.Wang, ZH, Guo, HB, et al.The esifundweni phakathi kwesifo se-fecal calamine nesifo sokuvuvukala samathumbu [j]. Ubuchwepheshe beSayensi nobunjiniyela, ngo-2010-03,10 (8)

Ukhiye wezimpawu ezisetshenzisiwe:

	Kudivayisi yezokwelapha yokuxilongwa kwe-vitro
	Umenzi
	Gcina ngo-2-30 ℃
	Usuku lokuphelelwa isikhathi
	Ungaphinde usebenzise kabusha
	Isiqapheliso
	Bheka imiyalo yokusebenzisa

Xiamen Wiz Biotech Co., Ltd
Ikheli: 3-4 phansi, i-No.16 yokwakha, indawo yokusebenzela ye-bio-medical, 2030 wengjiao West Road, Haicang District, 3610266, xiamen, China
I-TELE: + 86-592-6808278
Ifeksi: + 86-592-6808279