Ikhithi yokuxilonga ye-Antibody Subtype kuya ku-Helicobacter Pylori
Ulwazi lokukhiqiza
Inombolo Yemodeli | HP-ab-s | Ukupakisha | 25 Ukuhlolwa/ikhithi, 30kits/CTN |
Igama | I-Antibody Subtype kuya ku-Helicobacter Pylori | Ukuhlukaniswa kwezinsimbi | Ikilasi I |
Izici | Ukuzwela okuphezulu, ukusebenza okulula | Isitifiketi | CE/ ISO13485 |
Ukunemba | > 99% | Impilo yeshelufu | Iminyaka Emibili |
Indlela yokwenza | I-Fluorescence Immunochromatographic Assay | Isevisi ye-OEM/ODM | Iyatholakala |
Isifinyezo
I-Helicobacter pylori amabhaktheriya ane-gram-negative, futhi ukuma okugobile okugobile kuyinikeza igama elithi helicobacterpylori. I-Helicobacter pylori ihlala ezindaweni ezahlukene zesisu kanye ne-duodenum, okuzoholela ekuvuvukeni okungapheli kwe-gastric mucosa, izilonda zesisu kanye ne-duodenal, nomdlavuza wesisu. I-International Agency for Research on Cancer yahlonza ukutheleleka kwe-HP njenge-Class I carcinogen ngo-1994, kanti i-cancerogenic HP iqukethe ikakhulukazi ama-cytotoxins amabili: eyodwa i-cytotoxin-associated CagA protein, enye i-vacuolating cytotoxin (VacA). I-HP ingahlukaniswa ngezinhlobo ezimbili ngokusekelwe ekuboniseni kwe-CagA ne-VacA: uhlobo lwe-I luwuhlobo lwe-toxigenic (ngokuveza kokubili i-CagA ne-VacA noma enye yazo), okuyi-pathogenic kakhulu futhi kulula ukuyibangela izifo zesisu; uhlobo II luyi-atoxigenic HP (ngaphandle kokuvezwa kwakho kokubili i-CagA ne-VacA), engenabo ubuthi obuncane futhi ngokuvamile engenazo izimpawu zomtholampilo lapho ungenwa isifo.
Isici:
• Ukuzwela okuphezulu
• umphumela wokufunda emizuzwini eyi-15
• Ukusebenza okulula
• Intengo eqondile yasefekthri
• dinga umshini wokufunda umphumela
Ihlose Ukusebenzisa
Le kit isetshenziswa ekutholweni kwekhwalithi ye-in vitro ye-Urease antibody, i-CagA antibody kanye ne-VacA antibody ku-helicobacter pylori egazini lomuntu lilonke, isampula ye-serum noma i-plasma, futhi ilungele ukuhlonzwa okusizayo kokutheleleka nge-HP kanye nokuhlonza uhlobo lwesiguli se-helicobacter pylori. abathelelwe okuthi. Le khithi inikeza kuphela imiphumela yokuhlolwa ye-Urease antibody, i-CagA antibody ne-VacA antibody ku-helicobacter pylori, futhi imiphumela etholiwe izosetshenziswa ngokuhambisana nolunye ulwazi lomtholampilo ukuze luhlaziywe. Kufanele isetshenziswe kuphela ochwepheshe bezempilo.
Inqubo yokuhlola
1 | I-1: Ukusetshenziswa kwe-immune analyzer ephathekayo |
2 | Vula iphakheji yesikhwama se-aluminium foil ye-reagent bese ukhipha idivayisi yokuhlola. |
3 | Faka ngokuvundlile idivayisi yokuhlola ku-slot ye-immune analyzer. |
4 | Ekhasini lasekhaya le-interface yokusebenza ye-immune analyzer, chofoza okuthi "Standard" ukuze ufake isixhumi esibonakalayo sokuhlola. |
5 | Chofoza okuthi “QC Scan” ukuze uskene ikhodi ye-QR ohlangothini lwangaphakathi lwekhithi; amapharamitha ahlobene nekhithi ethuluzini bese ukhetha uhlobo lwesampula.Qaphela: Inombolo ngayinye yeqoqo lekhithi izoskenwa ngesikhathi esisodwa. Uma inombolo ye-batch iskeniwe, ke yeqa lesi sinyathelo. |
6 | Hlola ukuvumelana "Kwegama Lomkhiqizo", "Inombolo Yeqoqo" njll. kusixhumi esibonakalayo sokuhlola ngolwazi olukulebula yekhithi. |
7 | Qala ukungeza isampula uma kwenzeka kuba nolwazi olungaguquki:Isinyathelo 1: kancane kancane i-pipette 80μL serum/plasma/isampula yegazi lonke ngesikhathi esisodwa, futhi unake amabhamuza e-pipette; Isinyathelo sesi-2: isampula ye-pipette ukuze wenze isampula ye-diluent, futhi uhlanganise kahle isampula ne-diluent eyisampula; Isinyathelo sesi-3: isixazululo se-pipette 80µL esixutshwe kahle emthonjeni wedivayisi yokuhlola, futhi unake ukuthi cha kumabhamuza e-pipette ngesikhathi sokusampula |
8 | Ngemva kokufakwa okuphelele kwesampula, chofoza "Isikhathi" futhi isikhathi sokuhlola esisele sizovezwa ngokuzenzakalelayo kusixhumi esibonakalayo. |
9 | I-immune analyzer izoqedela ukuhlola nokuhlaziya ngokuzenzakalelayo lapho isikhathi sokuhlolwa sesifinyelelwe. |
10 | Ngemva kokuqedwa kokuhlolwa kwe-immune analyzer, umphumela wokuhlolwa uzovezwa kusixhumi esibonakalayo sokuhlola noma ungabukwa “Ngomlando” ekhasini lasekhaya lesixhumi esibonakalayo sokusebenza. |