I-Diagnostic Kit ye-Alpha-fetoprotein(Fleorescence Immunochrographic Assay)
Ngokusetshenziswa kokuxilongwa kwe-vitro kuphela
Uyacelwa ukuthi ufunde leli phakethe Faka ngokucophelela ngaphambi kokusebenzisa futhi ulandele imiyalo ngokuqinile. Ukwethembeka kwemiphumela ye-Assay akunakuqinisekiswa uma kukhona ukuphambuka emiyalweni ekuleW kufakiwe.

Ukusetshenziswa okuhlosiwe

Ikhithi yokuxilonga ye-Alpha-fetoprotein (Fleorescence Immunochrographic Assay i-fluorescence immunochrographic assay for the serum auxitative of Alpha-fetoprotein (AFP) ku-auxitissies (afpma, esetshenziselwa ukufunwa kwe-hepatocellular Carcinoma. Konke amasampula amahle kufanele kuqinisekiswe ezinye izindlela. Lokhu kuhlola kuhloselwe ukusetshenziswa kwezempilo kuphela.

Ukubeka kafushane

I-Alpha-fetoprotein (AFP) ingenye yezimpawu ezivame ukusetshenziswa yi-tumor.it iyi-glycoprotein enesisindo samangqamuzana esingu-70,000 noshukela we-4% .Ikususwa ngesibindi se-fetus, kulandelwa yi-yolk sac.I fetus yaqala ukuhlanganisa Amaviki ayi-6, afinyelela inani eliphakeme lamaviki ayi-12 kuye kwayi-15, ukugxilwa kwe-serum okungu-1 kuye ku-3 g / l, kanye ne-umbilical ged igazi lapho kuzalwa iminyaka eyi-100; ukukhulelwa okujwayelekile kungafinyelela 90 kuya ku-500 ng / ml phakathi; okuqukethwe okujwayelekile kwe-serum afp afp kuphakathi kuka-2 no-8 ng / ml, kepha izifo eziningi, ikakhulukazi i-hepatitis, zithinta inani le-AFP.

Umgomo wenqubo

Ulwelwesi lwedivayisi yokuhlola luhlanganiswe ne-anti AFP antibody kwisifunda sokuhlola kanye nembuzi anti Unogwaja IGG Antibody on the control. I-Lable Pad ihlanganiswe yi-fluorescence ilebula anti antp antibody kanye ne-rabbit igg kusengaphambili. Lapho uhlola isampula elinethemba, i-antigen ye-AFP in isampula ihlangana ne-fluorescence ilebula anti antibod antibod, bese wenza umxube wokuzivikela. Ngaphansi kwesenzo se-Immunochrotography, ukugeleza okuyinkimbinkimbi kokuqondisa kwephepha lokungena, lapho kudluliswe isikhathi sokuhlola, kuhlanganiswe ne-anti afp coating antilod, amafomu amasha acwebile.AfP axhumeka kahle ngesiginali ye-fluorescence, kanye ne-afp Kusampula kungatholwa yi-fluorencem immunoassay assay.

Ama-reagents nezinto zokwenziwa

25T Iphakethe Izakhi:

.Test Card ngakunye i-foil ipaniwe nge-desiccant 25t
.Sample Izimbamba 25t
.Fackage Faka 1

Izinto ezidingekayo kodwa azinikeziwe
Isithakongo esiqoqiwe sesampula, isibali sikhathi
Ukuqoqwa kwesampula nesitoreji
1.Izimpumu ezihlolwe zingaba yi-serum, i-heparin anticoaagulant plasma noma i-edta anticoaagulant plasma.
2.Faka kumasu ajwayelekile ukuqoqa isampula. Isampula ye-serum noma ye-plasma ingagcinwa esiqandisini ku-2-8 ℃ ngezinsuku eziyi-7 days kanye ne-cropressoal
I-3.all isampula igweme imijikelezo ye-freeze-thaw.

Inqubo yokufa
Uyacelwa ukuthi ufunde incwadi yokusebenza kwensimbi yokusebenza kwensimbi kanye nokufaka iphakheji ngaphambi kokuhlolwa.
1.Lay ubeka wonke ama-reagents namasampula wokushisa kwegumbi.
I-2.open them immune analyzer (Wiz-A101), faka ukungena ngemvume kwe-akhawunti ngokusho kwendlela yokusebenza yensimbi, bese ufaka isikhombimsebenzisi sokuthola.
I-3.Sc Ikhodi Yokucacisa Ukuqinisekisa Into Yokuhlola.
4.Thola ikhadi lokuhlola esikhwameni se-foil.
5.Shuma ikhadi lokuhlola ekhadini lekhadi, scan ikhodi ye-QR, bese unquma into yokuhlola.
I-6.Add 20μL serum noma isampula ye-plasma ukuze isampula isampula, uhlanganise kahle ..
7.ADD 80μL isixazululo isampula yesampula kahle yekhadi.
8.Chofoza inkinobho ethi "Ukuhlolwa okujwayelekile", ngemuva kwemizuzu eyi-15, ithuluzi lizobona ngokuzenzakalelayo ikhadi lokuhlola, lingafunda imiphumela kusuka esikrinini sokubonisa sensimbi, bese uqopha / phrinta imiphumela yokuhlola.
9.Refer ekufundisweni kwe-immune analyzer (Wiz-A101).

Amanani alindelwe

I-AFP: <10ng / ml
Kunconywa ukuthi ilebhu ngalinye ngalinye lasungulwa uhla lwalo olujwayelekile olumele abantu balo.

Imiphumela yokuhlola nokutolika
.Imininingwane engenhla ingumphumela wokuhlolwa kwe-afp ​​reagent, futhi kuphakanyiswa ukuthi ilabhorethri ngalinye kufanele lisungule uhla lwamanani wokutholwa kwe-AFP alungele inani labantu kulesi sifunda. Imiphumela engenhla ingeyokubhekiswa kuphela.
.Imiphumela yale ndlela isebenza kuphela kumabanga okuthola amareferensi asungulwe kule ndlela, futhi akukho ukuqhathanisa okuqondile nezinye izindlela.
Izici ze-.

Ukugcinwa nokuqina
I-1.Ikhithi iyizinyanga eziyi-18 zeShelf-Life kusuka ngosuku lokukhiqiza. Gcina amakhithi angasetshenziswanga ngo-2-30 ° C. Ungabazi. Ungasebenzisi ngaphezu kosuku lokuphelelwa yisikhathi.
2.Ungakuvuli esikhwameni esivaliwe uze ulungele ukwenza isivivinyo, futhi ukuhlolwa okusetshenziswa okukodwa kuphakanyiswa ukuthi kusetshenziswe ngaphansi kwendawo edingekayo (lokushisa 2-35 ℃, umswakama we-40-90%) ngaphakathi kwamaminithi angama-60 ngokushesha ngangokunokwenzeka.
3.Sample Diloulent isetshenziswa ngokushesha ngemuva kokuvulwa.

Izexwayiso kanye nokuqapha
.Ikhithi kufanele ivalwe futhi ivikelwe kumswakama.
.Imininingwane emihle izoqinisekiswa ezinye izindlela.
.All amasampula azophathwa njengokungcoliswa okungaba khona.
.Ungasebenzisi i-reagent ephelelwe yisikhathi.
. Hhayi ukushintshana kwama-reagents phakathi kwamakhithi ane-Lot ehlukile Cha ..
.Ungasebenzisi kabusha amakhadi okuhlola nanoma yiziphi izesekeli ezilahlwayo.
.Kungani, isampula ngokweqile noma elincane lingaholela ekuphaphemeni komphumela.

LUkufanisa
.S nganoma yisiphi ama-antibodies asegulayo e-Assay asebenzisa, kungenzeka ukuthi kuphazanyiswe ngama-antibodies angomuntu alwa nobuntu (Hama) ku-specimen. Izinhlobo ezivela ezigulini ezithole amalungiselelo ama-antibodies e-monoclonal yokuxilongwa noma ukwelashwa kungaqukatha uHama. Izinhlobo ezinjalo zingadala imiphumela emibi noma engamanga.
Umphumela wokuhlola we-.Lokhuwo we-. .
.Lokhu i-realent isetshenziselwa kuphela ukuhlolwa kwe-serum ne-plasma. Kungase kungatholi umphumela onembile uma usetshenziselwa amanye amasampula anjenge-saliva nomchamo nokunye.

Izici Zokusebenza

REcabanga
1.Hansen JH, et al.hama ukuphazamiseka nge-murine monoclonal antibody-based immunody [j] .j we-Clin immunoassay, 1993,16: 294-299.
I-2.levinson ss.Imvelo yama-antibophilies ama-heterophilic kanye neqhaza ekuphazanyisweni kwe-immunoassay [j] .j we-mimunoassay, 1992,15: 108-114.

Ukhiye wezimpawu ezisetshenzisiwe:

	Kudivayisi yezokwelapha yokuxilongwa kwe-vitro
	Umenzi
	Gcina ngo-2-30 ℃
	Usuku lokuphelelwa isikhathi
	Ungaphinde usebenzise kabusha
	Isiqapheliso
	Bheka imiyalo yokusebenzisa

Xiamen Wiz Biotech Co., Ltd
Ikheli: 3-4 phansi, i-No.16 yokwakha, indawo yokusebenzela ye-bio-medical, 2030 wengjiao West Road, Haicang District, 3610266, xiamen, China
I-TELE: + 86-592-6808278
Ifeksi: + 86-592-6808279