Ikhithi yokuxilonga ye-25-hydroxy vithamin d(Fleorescence Immunochrographic Assay)
Ngokusetshenziswa kokuxilongwa kwe-vitro kuphela
Uyacelwa ukuthi ufunde leli phakethe Faka ngokucophelela ngaphambi kokusebenzisa futhi ulandele imiyalo ngokuqinile. Ukwethembeka kwemiphumela ye-Assay akunakuqinisekiswa uma kukhona ukuphambuka emiyalweni ekuleW kufakiwe.

Ukusetshenziswa okuhlosiwe
I-Diagnostic Vitamin D ye-25-hydroxy Vitamin D (Fleorescence Immunochrogamographic Assay is a Fleorescence Immunochrographic assay for ardroochy vithamin d (25- (oh) vd) ku-serum yomuntu noma i-plasma, esetshenziselwa ikakhulukazi ukuhlola amazinga Ku-Vitamin D.It kuyinto yokuxilongwa okusizayo.Izinsuku ezinhle zesampula kufanele ziqinisekiswe ezinye izindlela. Lokhu kuhlola kuhloselwe ukusetshenziswa kwezempilo kuphela.

Ukubeka kafushane
UVitamin D uyi-Vitamin futhi futhi uyi-hormone ye-steroid, ikakhulukazi kufaka phakathi i-VD2 ne-VD3, intambo yawo ifana kakhulu. UVitamin D3 no-D2 baguqulwa baba ngu-25 hydroxyl Vitamin D (kufaka phakathi i-25-Dihydroxyl Vitamin D3 ne-D2). 25- (oh) vd emzimbeni womuntu, ekwakheni okuzinzile, okuhlushwa okuphezulu. 25- (oh) vd ukhombisa inani eliphelele le-Vitamin D, kanye nekhono lokuguqulwa kwe-Vitamin D, ngakho-ke 25- (oh) vd kubhekwa njengenkomba engcono kakhulu yokuhlola izinga le-Vitamin D.Ikhithi yokuxilonga isuselwe ku I-Immunochrotography futhi inganikeza umphumela kungakapheli imizuzu eyi-15.

Umgomo wenqubo
Ulwelwesi lwedivayisi yokuhlola luhlanganiswe ne-conjugate ye-BSA no-25- (oh) vd esifundeni sokuhlola kanye nembuzi anti unogwaja IGG antibody on the control. I-Marker Pad ihlanganiswe ngu-Fluorescence Mark anti 25- (oh) VD antibody kanye noRabbit IGG kusengaphambili. Lapho uhlola isampula, 25- (oh) vd ngesampula ehlangana ne-fluorescence emakwe anti 25- (oh) vd antibody, bese wenza ingxube yomzimba. Ngaphansi kwesenzo se-Immunochrotography, ukugeleza okuyinkimbinkimbi kokuqondiswa kwephepha lokumunca, lapho i-Complex idlulisela isifunda sokuhlola, umaki wamahhala we-fluorescent uzohlanganiswa ne-25- (oh) VD ku-membrane.I-ah) I-VD ukuxhumeka okungalungile kwesiginali ye-fluorescence, kanye nokuhlushwa okungu-25- (oh) vd ngesampula yesampula kungatholwa yi-fluorencem immunoassay assay.

Ama-reagents nezinto zokwenziwa

25T Iphakethe Izakhi:
.Test Card ngakunye i-foil ipaniwe nge-desiccant 25t
.A Isixazululo 25t
.B Isixazululo 1
.Fackage Faka 1

Izinto ezidingekayo kodwa azinikeziwe
Isithakongo esiqoqiwe sesampula, isibali sikhathi

Ukuqoqwa kwesampula nesitoreji
1.Izimpumu ezihlolwe zingaba yi-serum, i-heparin anticoaagulant plasma noma i-edta anticoaagulant plasma.
2.Faka kumasu ajwayelekile ukuqoqa isampula. Isampula ye-serum noma ye-plasma ingagcinwa esiqandisini ku-2-8 ℃ ngezinsuku eziyi-7 days kanye ne-cropressoal
I-3.all isampula igweme imijikelezo ye-freeze-thaw.

Inqubo yokufa
Inqubo yokuhlola ithuluzi ibona incwajana ye-immunoanalyzer. Inqubo yokuhlola i-Reagent imi kanjena
1.Lay ubeka wonke ama-reagents namasampula wokushisa kwegumbi.
I-2.open them immune analyzer (Wiz-A101), faka ukungena ngemvume kwe-akhawunti ngokusho kwendlela yokusebenza yensimbi, bese ufaka isikhombimsebenzisi sokuthola.
I-3.Sc Ikhodi Yokucacisa Ukuqinisekisa Into Yokuhlola.
4.Thola ikhadi lokuhlola esikhwameni se-foil.
5.Shuma ikhadi lokuhlola ekhadini lekhadi, scan ikhodi ye-QR, bese unquma into yokuhlola.
I-6.Add 30μL serum noma isampula ye-plasma kwikhambi, uhlanganise kahle.
7.ADD 50μL B Isixazululo sengxube engenhla, uhlanganise kahle.
8.Shiya ingxube imizuzu engu-15.
9.Add 80μl ingxube yesampula kahle ekhadini.
10.Chofoza inkinobho ethi "Ukuhlolwa okujwayelekile", ngemuva kwemizuzu eyi-10, ithuluzi lizothola ngokuzenzakalelayo ikhadi lokuhlola, lingafunda imiphumela kusuka esikrinini sokubonisa sensimbi, bese uqopha / phrinta imiphumela yokuhlola.
11.Refer ekufundisweni kwe-immune analyzer (Wiz-A101).

Amanani alindelwe
25- (oh) Ububanzi obujwayelekile be-VD: 30-100NG / ML
Kunconywa ukuthi ilebhu ngalinye ngalinye lasungulwa uhla lwalo olujwayelekile olumele abantu balo.

Imiphumela yokuhlola nokutolika
.Imininingwane engenhla iyisikhashana esisungulwe idatha yokutholwa kwale kit, futhi kuphakanyiswa ukuthi ilabhorethri ngalinye kufanele lisungule isikhawu sokubaluleka komtholampilo.
.Ukuhlushwa okungu-25- (oh) vd kuphakeme kunebanga lereferensi, futhi izinguquko zomzimba noma impendulo yokucindezela kufanele zikhishwe ngaphandle kwe-
.Imiphumela yale ndlela isebenza kuphela ebangeni lereferensi elisungulwe yile ndlela, futhi imiphumela ayiqhathaniswa ngqo nezinye izindlela.
Izici ze-.

Ukugcinwa nokuqina
.I-Kit iyinyanga engu-18 izinyanga zeshelufu kusuka ngosuku lokwenziwa. Gcina amakhithi angasetshenziswanga ngo-2-30 ° C. Ungabazi. Ungasebenzisi ngaphezu kosuku lokuphelelwa yisikhathi.
.Ungavuli esikhwameni esivaliwe uze ulungele ukwenza isivivinyo, futhi ukuhlolwa okukodwa kuphakanyiswa ukuthi kusetshenziswe ngaphansi kwendawo edingekayo (lokushisa 2-35 ℃, umswakama u-40-90%) ngaphakathi kwamaminithi angama-60 ngokushesha nje kungenzeka.
.Sample Dilount isetshenziswa ngokushesha ngemuva kokuvulwa.

Izexwayiso kanye nokuqapha
.Ikhithi kufanele ivalwe futhi ivikelwe kumswakama.
.Imininingwane emihle izoqinisekiswa ezinye izindlela.
.All amasampula azophathwa njengokungcoliswa okungaba khona.
.Ungasebenzisi i-reagent ephelelwe yisikhathi.
.Danga ukuguqula ama-reagents phakathi kwama-kits ane-lot ehlukile cha ..
.Ungasebenzisi kabusha amakhadi okuhlola nanoma yiziphi izesekeli ezilahlwayo.
.Kungani, isampula ngokweqile noma elincane lingaholela ekuphaphemeni komphumela.

LUkufanisa
.S nganoma yisiphi ama-antibodies asegulayo e-Assay asebenzisa, kungenzeka ukuthi kuphazanyiswe ngama-antibodies angomuntu alwa nobuntu (Hama) ku-specimen. Izinhlobo ezivela ezigulini ezithole amalungiselelo ama-antibodies e-monoclonal yokuxilongwa noma ukwelashwa kungaqukatha uHama. Izinhlobo ezinjalo zingadala imiphumela emibi noma engamanga.
Umphumela wokuhlola we-.Lokhuwo we-. .
.Lokhu i-realent isetshenziselwa kuphela ukuhlolwa kwe-serum ne-plasma. Kungase kungatholi umphumela onembile uma usetshenziselwa amanye amasampula anjenge-saliva nomchamo nokunye.

Izici Zokusebenza

REcabanga

1.Hansen JH, et al.hama ukuphazamiseka nge-murine monoclonal antibody-based immunody [j] .j we-Clin immunoassay, 1993,16: 294-299.
I-2.levinson ss.Imvelo yama-antibophilies ama-heterophilic kanye neqhaza ekuphazanyisweni kwe-immunoassay [j] .j we-mimunoassay, 1992,15: 108-114.

Ukhiye wezimpawu ezisetshenzisiwe:

	Kudivayisi yezokwelapha yokuxilongwa kwe-vitro
	Umenzi
	Gcina ngo-2-30 ℃
	Usuku lokuphelelwa isikhathi
	Ungaphinde usebenzise kabusha
	Isiqapheliso
	Bheka imiyalo yokusebenzisa

Xiamen Wiz Biotech Co., Ltd
Ikheli: 3-4 phansi, i-No.16 yokwakha, indawo yokusebenzela ye-bio-medical, 2030 wengjiao West Road, Haicang District, 3610266, xiamen, China
I-TELE: + 86-592-6808278
Ifeksi: + 86-592-6808279