ISIMISO

I-membrane yedivayisi yokuhlola ihlanganiswe ne-conjugate ye-BSA kanye ne-25-(OH)VD endaweni yokuhlola kanye ne-anti-rabbit IgG ye-antibody yembuzi endaweni yokulawula. Iphedi lomaka limbozwe nge-fluorescence mark anti-25-(OH)VD antibody kanye ne-IgG yonogwaja kusengaphambili. Lapho kuhlolwa isampula, i-25-(OH)VD kusampula ihlangana ne-fluorescence ephawulwe ukuthi i-anti 25-(OH)VD antibody, bese yenza ingxube yamasosha omzimba. Ngaphansi kwesenzo se-immunochromatography, ukugeleza okuyinkimbinkimbi kubheke ephepheni elimuncayo, lapho okuyinkimbinkimbi kudlula isifunda sokuhlola, Umaka we-fluorescent wamahhala uzohlanganiswa no-25-(OH)VD kulwelwesi.Ukuhlushwa okungu-25-(OH) I-VD iwukuhlobana okunegethivu kwesignali ye-fluorescence, futhi ukugxila kwe-25-(OH)VD kusampula kungatholwa nge-fluorescence immunoassay assay.

Inqubo Yokuhlola:

Sicela ufunde imanuwali yokusebenza kwezinsimbi kanye nokufakwa kwephakheji ngaphambi kokuhlola.

1. Beka eceleni wonke ama-reagents namasampuli ekamelweni lokushisa.
2. Vula i-Portable Immune Analyzer(WIZ-A101), faka ukungena ngemvume kwephasiwedi ye-akhawunti ngokwendlela yokusebenza yensimbi, bese ufaka isixhumi esibonakalayo sokubona.
3. Skena ikhodi yokuhlonza ukuze uqinisekise into yokuhlola.
4. Khipha ikhadi lokuhlola esikhwameni se-foil.
5. Faka ikhadi lokuhlola endaweni yekhadi, skena ikhodi ye-QR, bese unquma into yokuhlola.
6. Faka i-serum engu-15μL noma isampula ye-plasma ibe isixazululo, uhlanganise kahle
7. Engeza ingxube engu-80μL ukuze usampula kahle ikhadi.
8. Chofoza inkinobho ethi "ukuhlolwa okujwayelekile", ngemuva kwemizuzu eyi-15, ithuluzi lizothola ngokuzenzakalelayo ikhadi lokuhlola, lingakwazi ukufunda imiphumela esikrinini sokubonisa sensimbi, futhi lirekhode/liphrinte imiphumela yokuhlolwa.
9. Bheka imiyalelo ye-Portable Immune Analyzer(WIZ-A101).