Ikhithi yokuxilonga(Igolide le-Colloidal）i-Luteinizing Hormone
Ukusetshenziswa kwe-in vitro diagnostic kuphela
Sicela ufunde le phakheji ngokucophelela ngaphambi kokuyisebenzisa futhi ulandele ngokuqinile imiyalelo. Ukuthembeka kwemiphumela yokuhlolwa akunakuqinisekiswa uma kukhona ukuchezuka emiyalweni ekule kokufakwa kwephakheji.

UKUSETSHENZISWA OKUHLOSIWE

Ikhithi isetshenziselwa ukutholwa kwekhwalithi yamazinga e-Luteinizing Hormone (LH) kumasampula omchamo womuntu. Kufanelekile ukubikezela isikhathi se-ovulation. Qondisa abesifazane abaseminyakeni yokubeletha ukuze bakhethe isikhathi esingcono kakhulu sokukhulelwa, noma uqondise ukuvimbela inzalo okuphephile.Lokhu kuhlola kuyisici sokuhlola. Wonke amasampula amahle kufanele aqinisekiswe ngezinye izindlela. Lokhu kuhlolwa kuhloselwe ukusetshenziswa ngochwepheshe bezempilo kuphela. Okwamanje, lokhu kuhlolwa kusetshenziselwa i-IVD, izinsimbi ezengeziwe azidingeki.

USIZE WEPHAKEJI

Ikhithi engu-1/ibhokisi, amakhithi ayi-10/ibhokisi, amakhithi angama-25,/ibhokisi, amakhithi ayi-100/ibhokisi.

ISIFINYEZO
I-LH iyi-hormone ye-glycoprotein efihliwe yi-pituitary gland, ikhona egazini lomuntu nomchamo, ongakhuthaza ukukhululwa kwamaqanda avuthiwe ku-ovary. I-LH ikhiqizwa phakathi nesikhathi esimaphakathi sokuya esikhathini, futhi ukwakheka kwe-LH ephezulu, yakhuphuka ngokushesha yafinyelela ku-25-200 miu/mL isuka ezingeni eliyisisekelo lika-5-20 miu/mL. Ukugcwala kwe-LH emchameni kuvame ukukhuphuka okubukhali emahoreni angama-36-48 ngaphambi kokuvuthwa kweqanda, kufinyelela inani eliphakeme emahoreni ayi-14-28. Inani le-LH emchameni ngokuvamile lenyuka ngokucijile cishe emahoreni angu-36 kuya kwangu-48 ngaphambi kokuvuthwa kweqanda, futhi lafinyelela umvuthwandaba emahoreni angu-14 ~ 28, ulwelwesi lwe-follicular lwaqhuma cishe emahoreni angu-14 kuya kwangu-28 ngemva kokuphakama futhi lwakhipha amaqanda avuthiwe. Abesifazane bavunde kakhulu ekuphakameni kwe-LH phakathi nezinsuku ezingu-1-3, ngakho-ke, ukutholakala kwe-LH emchameni kungasetshenziswa ukubikezela isikhathi sokuvuthwa.^[1]. Le khithi esekelwe kubuchwepheshe bokuhlaziya i-colloidal immune chromatography ukuze kutholwe ikhwalithi ye-LH antigen kumasampula omchamo womuntu, okunganikeza umphumela phakathi nemizuzu eyi-15.

INQUBO YOKUHLOLA
1.Khipha ikhadi lokuhlola esikhwameni se-foil, ulibeke etafuleni lezinga bese ulimaka.
2.Lahla isampula yokuqala yamaconsi amabili, engeza amaconsi ama-3 (cishe u-100μL) akukho sampuli yebhamuza mpo futhi kancane ungene kwisampula yomthombo wekhadi nge-dispette enikeziwe, qala isikhathi.
3.Umphumela kufanele ufundwe phakathi kwemizuzu eyi-10-15, futhi awuvumelekile ngemuva kwemizuzu eyi-15.