Ikhithi Yokuxilonga (Igolide Le-Colloidal) yeGonadotrophin Yechorionic Yabantu
Ikhithi yokuxilonga(Igolide le-Colloidal)I-Gonadotrophin yeChorionic Yabantu
Ukusetshenziswa kwe-in vitro diagnostic kuphela
Sicela ufunde le phakheji ngokucophelela ngaphambi kokuyisebenzisa futhi ulandele ngokuqinile imiyalelo. Ukuthembeka kwemiphumela yokuhlolwa akunakuqinisekiswa uma kukhona ukuchezuka emiyalweni ekule kokufakwa kwephakheji.
UKUSETSHENZISWA OKUHLOSIWE
Ikhithi Yokuhlola (i-Colloidal Gold) ye-Human Chorionic Gonadotrophin iyi-colloidal gold immunochromatographic assay yokutholwa kwekhwalithi yamazinga e-chorionic gonadotropin (HCG) ku-serum yomuntu nomchamo, isetshenziselwa ukuxilongwa kokukhulelwa kusenesikhathi. Wonke amasampula amahle kufanele aqinisekiswe ngezinye izindlela. Lokhu kuhlolwa kuhloselwe ukusetshenziswa ngochwepheshe bezempilo kuphela.
USIZE WEPHAKEJI
Ikhithi engu-1/ibhokisi, amakhithi ayi-10/ibhokisi, amakhithi angama-25,/ibhokisi, amakhithi angama-50/ibhokisi.
ISIFINYEZO
I-HCG iyi-hormone ye-glycoprotein ekhishwa yi-placenta ekhulayo ngemva kokuvundiswa kweqanda. Amazinga e-HCG angakhushulwa ngokushesha ku-serum noma umchamo ekuqaleni kwamaviki angu-1 kuya kwangu-2.5 ngesikhathi sokukhulelwa, futhi afinyelele inani eliphakeme emasontweni angu-8, kunokuwela ezingeni eliphakathi ezinyangeni ezi-4, futhi agcinwe izinga kuze kube sekupheleni kokukhulelwa.[1]. Ikhithi iyisivivinyo esilula, esibonakalayo sekhwalithi esithola i-HCG antigen ku-serum yomuntu noma umchamo. Ikhithi Yokuxilonga isekelwe ku-immunochromatography futhi inganikeza umphumela phakathi nemizuzu eyi-15.
INQUBO YOKUHLOLA
1.Khipha ikhadi lokuhlola esikhwameni se-foil, ulibeke etafuleni lezinga bese ulimaka.
2.Lahla isampula yokuqala yamaconsi amabili, engeza amaconsi ama-3 (cishe u-100μL) akukho sampuli yebhamuza mpo futhi kancane ungene kwisampula yomthombo wekhadi nge-dispette enikeziwe, qala isikhathi.
3.Umphumela kufanele ufundwe phakathi kwemizuzu eyi-10-15, futhi awuvumelekile ngemuva kwemizuzu eyi-15.