Ikhithi yokuxilonga (igolide eliyi-Colloidal) ye-Follicle-stimulating Hormone
Ikhithi yokuxilonga(Igolide le-Colloidal)ye-Follicle-stimulating Hormone
Ukusetshenziswa kwe-in vitro diagnostic kuphela
Sicela ufunde le phakheji ngokucophelela ngaphambi kokuyisebenzisa futhi ulandele ngokuqinile imiyalelo. Ukuthembeka kwemiphumela yokuhlolwa akunakuqinisekiswa uma kukhona ukuchezuka emiyalweni ekule kokufakwa kwephakheji.
UKUSETSHENZISWA OKUHLOSIWE
Ikhithi isetshenziselwa ukutholwa kwekhwalithi yamazinga e-follicle-stimulating hormone (FSH) kumasampula omchamo. Ilungele ukusiza ekunqumeni ukubukeka kokunqamuka kokuya esikhathini kwabesifazane.
USIZE WEPHAKEJI
Ikhithi engu-1/ibhokisi, amakhithi ayi-10/ibhokisi, amakhithi angama-25,/ibhokisi, amakhithi angama-50/ibhokisi.
ISIFINYEZO
I-FSH iyi-hormone ye-glycoprotein ekhiqizwa indlala ye-pituitary, ingangena egazini nomchamo ngokujikeleza kwegazi. Kwabesilisa, i-FSH ikhuthaza ukuvuthwa kwe-testicular seminiferous tubule kanye nokukhiqizwa kwesidoda, kowesifazane, i-FSH ikhuthaza ukukhula kwe-follicular nokuvuthwa, futhi isebenzisana ne-LH ku-follicles evuthiwe ikhiqiza i-estrogen kanye ne-ovulation, ehilelekile ekwakhekeni kokuya esikhathini okuvamile [1]. I-FSH igcina izinga le-basal elizinzile ezifundweni ezijwayelekile, mayelana no-5-20mIU/mL. Ukunqamuka kokuya esikhathini kwabesifazane ngokuvamile kwenzeka phakathi kweminyaka engama-49 no-54, futhi kuthatha isilinganiso seminyaka emine kuya kwemihlanu. Ngalesi sikhathi, ngenxa ye-ovarian atrophy, i-follicular atresia kanye nokuwohloka, ukukhiqizwa kwe-estrogen kwehle kakhulu, inani elikhulu lokukhishwa kwe-pituitary gonadotropin evuselelayo, ikakhulukazi amazinga e-FSH azokwenyuka kakhulu, ngokuvamile angu-40-200mIU/ml, futhi alondoloze izinga ku-pituitary gland. isikhathi eside kakhulu[2]. Le khithi esekelwe kubuchwepheshe bokuhlaziya i-colloidal immune chromatography ukuze kutholwe ikhwalithi ye-FSH antigen kumasampuli omchamo womuntu, okunganikeza umphumela phakathi nemizuzu engu-15.
INQUBO YOKUHLOLA
1.Khipha ikhadi lokuhlola esikhwameni se-foil, ulibeke etafuleni lezinga bese ulimaka.
2.Lahla isampula yokuqala yamaconsi amabili, engeza amaconsi ama-3 (cishe u-100μL) akukho sampuli yebhamuza mpo futhi kancane ungene kwisampula yomthombo wekhadi nge-dispette enikeziwe, qala isikhathi.
3.Umphumela kufanele ufundwe phakathi kwemizuzu eyi-10-15, futhi awuvumelekile ngemuva kwemizuzu eyi-15.