Kit diagnostic(Igolide le-colloidalIsihlehlukeneeCalprotectin
Ngokusetshenziswa kokuxilongwa kwe-vitro kuphela
Uyacelwa ukuthi ufunde leli phakethe Faka ngokucophelela ngaphambi kokusebenzisa futhi ulandele imiyalo ngokuqinile. Ukwethembeka kwemiphumela ye-Assay akunakuqinisekiswa uma kukhona ukuphambuka emiyalweni ekuleW kufakiwe.

Ukusetshenziswa okuhlosiwe
I-Diagnostic Kit yeCalprotectin (Cal) iyi-colloidal yegolide ye-immunochrographic assay design demiquantitative desure of cal kusuka kumalondolo abantu, anenani elibalulekile lokuthola izifo zamathumbu. Lokhu kuhlola kuyindlela yokuhlola. Konke amasampula amahle kufanele kuqinisekiswe ezinye izindlela. Lokhu kuhlola kuhloselwe ukusetshenziswa kwezempilo kuphela. Ngaleso sikhathi, lokhu kuhlolwa kusetshenziselwa i-IVD, amathuluzi angeziwe awadingeki.

Ukubeka kafushane
I-CAL iyi-heterodimer, eyakhiwa i-MRP 8 ne-MRP 14. Ikhona kuma-neutrophils cytoplasm futhi ivezwe ngolwelwesi lwamaseli we-mononuclear. I-CAL ingamaprotheni esigaba esibi, inesigaba esizinzile esiseduze kwesonto elilodwa kuma-faces abantu, izimisele ukuba ngumaka wezifo zamathumbu zamathumbu. I-KIT iyisivivinyo esilula, esibonakalayo se-semiqualitative esibona i-cal emalondolo antwini, inokuzwela okuphezulu nokucaciswa okuqinile. Ukuhlolwa okususelwa kuma-sandwich reaction antibodies aphezulu aphezulu kanye ne-Gold Immunochrochrographic assay ukuhlaziya ubuchwepheshe, kunganikeza umphumela kungakapheli imizuzu eyi-15.

Umgomo wenqubo
I-strip ine-anti cal coating MCAB kwisifunda sokuhlola kanye nembuzi anti-rabbit IGG antilody kwisifunda sokulawula, esifakwe ku-chromatography kusenesikhathi. I-Lable Pad ihlanganiswe ngegolide le-Colloidal legolide elibhalwe i-Anti Cal McAB kanye ne-colloidal igolide elibhalwe ukuthi unogwaja we-IGG antibody ngaphambi kwesikhathi. Lapho uhlola isampula elimuhle, i-cal kwisampula enamathele negolide elibhalwe ngegolide elibhalwe i-anti lungi, futhi litholakale lihambisane nomucu wokuhlola, i-Cal Conjugate Complex ithwetshiwe yi-anti cal coating MCAB kwi-membrane nefomu "I-Anti Cal Coating yegolide yelebula i-Cal-colloidal legolide anti Ukuqina kombala kuhlobene kahle nokuqukethwe kwekhwala. Isampula elingemuhle alikhiqizi ibhendi yokuhlola ngenxa yokungabikho kweColloidal Gold Comnjugate Cal Coblex. Akunandaba ukuthi i-CAL ikhona ngesampula noma cha, kukhona umugqa obomvu kuvela esifundeni sereferensi kanye nesifunda sokulawula esezingeni eliphezulu, okubhekwa njengekhwalithi yebhizinisi langaphakathi lebhizinisi langaphakathi.

Ama-reagents nezinto zokwenziwa
25T Iphakethe Izakhi:
.Test Card ngakunye icwebezela nge-desiccant
.Sample Izimbambandlela: Izithako zingu-20mm Ph7.4PS
.Dispette
.Fackage Faka

Izinto ezidingekayo kodwa azinikeziwe

Isithakongo esiqoqiwe sesampula, isibali sikhathi

Ukuqoqwa kwesampula nesitoreji
Sebenzisa isitsha esihlanzekile esilahlayo ukuqoqa isampula ye-Face Feeces, futhi ihlolwe ngokushesha. Uma kungeke kuhlolwe ngokushesha, sicela ugcinwe ku-2-8 ° C.ours noma i-bullel -15 ° C izinyanga ezi-4.

Inqubo yokufa
1. Thatha induku yesampula, ifakwe kwisampula ye-Faeces, bese ubeka induku yesampula emuva, isikulufa uqine futhi unikine kahle, phinda isenzo izikhathi ezi-3. Noma ukusebenzisa isampula elinamathele ku-50mg Faeces isampula, bese ufaka i-Faeces Samps Tube equkethe i-dilution yesampula, bese isikulufa ngokuqinile.
2.Sebenzisa isampula ye-Pipette elahlekile thatha isampula le-Finet Fisenes kusuka esigulini sohudo, bese ufaka amaconsi ama-3 (cishe i-100Ul) kwishubhu lesampula ye-fecal bese unikina kahle, ubeke eceleni.
3.ke ukhiphe ikhadi lokuhlola esikhwameni se-foil, ulibeke etafuleni lezinga bese umaka.
I-4.Remove cap kusuka ku-tube yesampula bese ulahla amasampula amabili okuqala ahlanjululwe, engeza amaconsi ama-3 (cishe u-100Ul) Ayikho i-bubble exutshwe isampula vertikelele futhi kancane kancane ekhadini elinikeziwe, qala isikhathi.
5.Umphumela kufanele ufundwe kungakapheli imizuzu eyi-10-15, futhi akulungile ngemuva kwemizuzu engu-15.

Imiphumela yokuhlola nokutolika

Ukugcinwa nokuqina
Ikhithi iyinyanga engama-24 izinyanga zeshalofu-impilo kusukela ngosuku lokukhiqiza. Gcina amakhithi angasetshenziswanga ngo-2-30 ° C. Ungavuli isikhwama esivaliwe uze ulungele ukwenza isivivinyo.
Izexwayiso kanye nokuqapha
I-1.Ikhithi kufanele ivalwe futhi ivikelwe kumswakama¹.
2.Ungasebenzisi isampula ebekwe isikhathi eside kakhulu noma esiphindaphindwayo futhi sihlanzekile ukuze uhlolwe
Amasampula we-3.Fecal ngokweqile noma ubukhulu angenza amasampulethi ahlasele angcolile, sicela uCentrifuge isampula ehlisiwe futhi uthathe umkhulumeli wokuhlola.
I-4.misoperAple, isampula ngokweqile noma elincane ingaholela ekuphaphemeni komphumela.

Ukukhawulelwa
Umphumela wokuhlola we-1.Laleli kuphela wereferensi emtholampilo kuphela, akufanele ukhonze njengeso kuphela isisekelo sokuxilongwa kokwelashwa nokwelashwa, ukuphathwa kwemitholampilo yeziguli kufanele kube ukucatshangelwa okuphelele okuhlanganiswe nezimpawu zalo, umlando wezokwelapha, okunye ukuhlolwa kwelebhu, ukuphendula kokwelashwa, ukuphendula kokwelashwa, ukuphendula kokwelashwa, i-epidemiology kanye nokunye ulwazi².
I-Recegent esetshenziselwa kuphela ukuhlolwa kwe-fecal. Kungase kungatholi umphumela onembile uma usetshenziselwa amanye amasampula anjenge-saliva nomchamo nokunye.