Ikhithi yokuxilonga ye-Procalcitonin

(ukuhlolwa kwe-fluorescence immunochromatographic)

Ukusetshenziswa kwe-in vitro diagnostic kuphela

Sicela ufunde le phakheji ngokucophelela ngaphambi kokuyisebenzisa futhi ulandele ngokuqinile imiyalelo. Ukuthembeka kwemiphumela yokuhlolwa akunakuqinisekiswa uma kukhona ukuchezuka emiyalweni ekule kokufakwa kwephakheji.

UKUSETSHENZISWA OKUHLOSIWE

I-Diagnostic Kit ye-Procalcitonin (i-fluorescence immunochromatographic assay) iwukuhlolwa kwe-fluorescence immunochromatographic ukuze kutholwe inani le-Procalcitonin (PCT) ku-serum yomuntu noma i-plasma, isetshenziselwa ukuxilongwa okuwusizo kokutheleleka kwebhaktheriya kanye ne-sepsis. Wonke amasampula amahle kufanele aqinisekiswe ngezinye izindlela. Lokhu kuhlolwa kuhloselwe ukusetshenziswa ngochwepheshe bezempilo kuphela.

ISIFINYEZO

I-Procalcitonin yakhiwe ama-amino acid ayi-116 futhi isisindo sayo samangqamuzana yi-12.7KD. I-PCT iboniswa ngamaseli e-neuroendocrine futhi adilizwa ama-enzyme abe yi-calcitonin (engavuthiwe), i-carboxy-terminating peptide, kanye ne-amino eqeda i-peptide. Abantu abanempilo banenani elincane le-PCT egazini labo, elingakhuliswa kakhulu ngemva kokutheleleka ngebhaktheriya. Uma i-sepsis ivela emzimbeni, izicubu eziningi zingaveza i-PCT, ngakho-ke i-PCT ingasetshenziswa njengenkomba yokubikezela ye-sepsis. Kwezinye iziguli ezinokutheleleka kokuvuvukala, i-PCT ingasetshenziswa njengenkomba yokukhetha ama-antibiotic kanye nokwahlulela okusebenzayo.

ISIMISO SENKQUBO

I-membrane yedivayisi yokuhlola ihlanganiswe ne-anti-PCT antibody endaweni yokuhlola kanye ne-anti-rabbit IgG ye-IgG yembuzi endaweni yokulawula. Iphedi elilebula limbozwa nge-fluorescence ebhalwe ukuthi anti-PCT antibody kanye ne-rabbit IgG kusengaphambili. Lapho kuhlolwa isampula elihle, i-antigen ye-PCT kusampula ihlangana ne-fluorescence ebhalwe ukuthi i-anti PCT antibody, bese yenza ingxube yokuzivikela komzimba. Ngaphansi kwesenzo se-immunochromatography, ukugeleza okuyinkimbinkimbi ekuqondeni kwephepha elimuncayo, lapho inkimbinkimbi idlula isifunda sokuhlola, ihlangene ne-anti-PCT yokuhlanganisa i-antibody, yakha inkimbinkimbi entsha. Izinga le-PCT lihlotshaniswa kahle nesignali ye-fluorescence, futhi ukugxila kwe-PCT kusampula kungatholwa nge-fluorescence immunoassay assay.