I-Diagnostic Kit ye-ProCalcitonin

(Fleorescence Immunochrographic Assay)

Ngokusetshenziswa kokuxilongwa kwe-vitro kuphela

Uyacelwa ukuthi ufunde leli phakethe Faka ngokucophelela ngaphambi kokusebenzisa futhi ulandele imiyalo ngokuqinile. Ukwethembeka kwemiphumela ye-Assay akunakuqinisekiswa uma kukhona ukuphambuka emiyalweni ekuleW kufakiwe.

Ukusetshenziswa okuhlosiwe

I-Diagnostic Kit ye-ProCallcitonin (Fleorescence Imunochrographic Assay i-fluorescence immunochrographic assay for the procalcitonin (PCTME) ku-serum yomuntu noma i-plasma, isetshenziselwa ukuxilongwa okusizayo kokutheleleka ngegciwane kanye ne-sepsis. Konke amasampula amahle kufanele kuqinisekiswe ezinye izindlela. Lokhu kuhlola kuhloselwe ukusetshenziswa kwezempilo kuphela.

Ukubeka kafushane

I-ProCALCITONIN yakhiwa ama-Amino acid angama-116 kanti isisindo sayo samangqamuzana singu-12.7kd. I-PCT ivezwa amaseli we-NeuroEendeocrine futhi aphulwe phansi ngama-enzyme angena (kungavuthiwe) i-calcitonin, i-carboxy-inquma i-peptide, kanye ne-amiptide ebheke phezulu. Abantu abaphilile banemali encane kuphela ye-PCT egazini labo, engakhuphuke kakhulu ngemuva kokutheleleka ngegciwane. Lapho i-Sepsis yenzeka emzimbeni, izicubu eziningi zingaveza i-PCT, ngakho-ke i-PCT ingasetshenziswa njengenkomba ye-sepsis. Kwezinye iziguli ezinokutheleleka kokuvuvukala, i-PCT ingasetshenziswa njengenkomba yokukhethwa kwamagciwane kanye nesahlulelo sokusebenza kahle.

Umgomo wenqubo

Ulwelwesi lwedivayisi yokuhlola luhlanganiswe ne-anti PCT antibody kwisifunda sokuhlola kanye nembuzi anti Unogwaja I-IGG antibody on the control. I-Lable Pad ihlanganiswe yi-fluorescence ilebula anti PCT antibody kanye noRabbit IGG kusengaphambili. Lapho uvivinya isampula eline-PCT, i-PCT Antigen yesampula ihlangana ne-fluorescence ilebula anti-pct antibody, bese wenza umxube wokuzivikela. Ngaphansi kwesenzo se-Immunochrotography, ukugeleza okuyinkimbinkimbi kokuqondiswa kwephepha lokumunca, lapho kuyinkimbinkimbi edlula esifundeni sokuhlola, kuhlanganiswe ne-anti PCT ehlanganisiwe i-antilody, amafomu amasha. Izinga le-PCT lixhumeka kahle ngesiginali ye-fluorescence, kanye nokuqoqwa kwe-PCT kwisampula yesampula kungatholwa yi-fluorence iMmunoassay Assay.