Ikhithi yokuxilonga ye-insulin yesifo sikashukela
Ikhithi yokuxilonga ye-insulin
Indlela yokusebenza:Fluorescence Immunochromatographic Assay
Ulwazi lokukhiqiza
Inombolo Yemodeli | I-INS | Ukupakisha | 25 Ukuhlolwa/ikhithi, 30kits/CTN |
Igama | Ikhithi yokuxilonga ye-insulin | Ukuhlukaniswa kwezinsimbi | Ikilasi Ii |
Izici | Ukuzwela okuphezulu, ukusebenza okulula | Isitifiketi | CE/ ISO13485 |
Ukunemba | > 99% | Impilo yeshelufu | Iminyaka Emibili |
Indlela yokwenza | I-Fluorescence Immunochromatographic Assay | Isevisi ye-OEM/ODM | Iyatholakala |
Ukuphakama
Isikhathi sokuhlola: 10-15mins
Isitoreji:2-30℃/36-86℉
Indlela yokusebenza:Fluorescence Immunochromatographic Assay
Isici:
• Ukuzwela okuphezulu
• umphumela wokufunda emizuzwini eyi-15
• Ukusebenza okulula
• Ukunemba okuphezulu
UKUSETSHENZISWA OKUHLOSIWE
Le kit ifanele i-in vitro quantitative determination of insulin (INS) ku-serum yabantu/i-plasma/amasampula egazi lonke ukuze kuhlolwe ukusebenza kwe-pancreatic-islet β-cell. Le kit ihlinzeka kuphela ngemiphumela yokuhlolwa kwe-insulin (INS), futhi umphumela otholiwe uzohlaziywa kuhlanganiswe nolunye ulwazi lomtholampilo. umphumela uzohlaziywa ngokuhambisana nolunye ulwazi lomtholampilo.
Inqubo yokuhlola
1 | Ngaphambi kokusebenzisa i-reagent, funda iphakheji ngokucophelela futhi uzijwayeze nezinqubo zokusebenza. |
2 | Khetha imodi yokuhlola evamile ye-WIZ-A101 ephathekayo yomzimba yokuhlaziya |
3 | Vula iphakheji yesikhwama se-aluminium foil ye-reagent bese ukhipha idivayisi yokuhlola. |
4 | Faka ngokuvundlile idivayisi yokuhlola ku-slot ye-immune analyzer. |
5 | Ekhasini lasekhaya le-interface yokusebenza ye-immune analyzer, chofoza okuthi "Standard" ukuze ufake isixhumi esibonakalayo sokuhlola. |
6 | Chofoza okuthi “QC Scan” ukuze uskene ikhodi ye-QR ohlangothini lwangaphakathi lwekhithi; amapharamitha ahlobene nekhithi ensimbini bese ukhetha uhlobo lwesampula. Qaphela: Inombolo ngayinye yeqoqo lekhithi izoskenwa ngesikhathi esisodwa. Uma inombolo ye-batch iskeniwe, bese weqa lesi sinyathelo. |
7 | Hlola ukuvumelana "Kwegama Lomkhiqizo", "Inombolo Yeqoqo" njll. kusixhumi esibonakalayo sokuhlola ngolwazi olukulebula yekhithi. |
8 | Khipha isampula encibilikisiwe olwazini olungaguquki, engeza u-10μL we-serum/plasma/isampula legazi lonke, bese uwaxuba kahle; |
9 | Engeza isixazululo esixutshwe kahle esingu-80µL esishiwo ngenhla emthonjeni wedivayisi yokuhlola; |
10 | Ngemva kokufakwa okuphelele kwesampula, chofoza "Isikhathi" futhi isikhathi sokuhlola esisele sizovezwa ngokuzenzakalelayo kusixhumi esibonakalayo. |
11 | I-immune analyzer izoqedela ukuhlola nokuhlaziya ngokuzenzakalelayo lapho isikhathi sokuhlolwa sesifinyelelwe. |
12 | Ngemuva kokuthi ukuhlolwa kwe-immune analyzer sekuqediwe, umphumela wokuhlolwa uzovezwa kusixhumi esibonakalayo sokuhlola noma ungabukwa “Ngomlando” ekhasini lasekhaya lesixhumi esibonakalayo sokusebenza. |
Qaphela: isampula ngayinye izofakwa ngepayipi ngepayipi elahlwayo elihlanzekile ukuze kugwenywe ukungcoliswa okuphambanayo.
Ukusebenza Komtholampilo
Ukusebenza kokuhlolwa komtholampilo kwalo mkhiqizo kwahlolwa ngokuqoqa amasampula omtholampilo angu-173. Imiphumela yokuhlolwa iqhathaniswe kusetshenziswa amakhithi ahambisanayo endlela ye-electrochemiluminescence emakethe njengamarejenti ereferensi, futhi ukuqhathaniswa kwawo kwaphenywa ngokuhlehla komugqa, futhi ama-coefficients okuxhumana alezi zivivinyo ezimbili kwaba ngu-y = 0.987x+4.401 kanye no-R = 0.9874, ngokulandelanayo. .