Ikhithi yokuhlola ye-cpn igm ikhithi yokuhlola ye-chlamydia pneumoniae igolide elicijile
Imikhiqizo Imingcele
ISIMISO NENKQUBO YOKUHLOLA I-FOB
ISIMISO
I-membrane yedivayisi yokuhlola ihlanganiswe ne-Cpn-IgM antibody endaweni yokuhlola kanye ne-anti-rabbit IgG ye-IgG yembuzi endaweni yokulawula. Iphedi elebulayo limbozwe nge-fluorescence ebhalwe ukuthi anti-Cpn-IgM antibody kanye ne-rabbit IgG kusengaphambili. Lapho kuhlolwa isampula elihle, i-antigen ye-Cpn-IgM kusampula ihlangana ne-fluorescence enelebula le-anti-Cpn-IgM antibody, bese yakhe ingxube yokuzivikela komzimba. Ngaphansi kwesenzo se-immunochromatography, ukugeleza okuyinkimbinkimbi kubheke ephepheni elimuncayo. Lapho inkimbinkimbi idlula isifunda sokuhlola, ihlanganiswe ne-anti-Cpn-IgM yokuhlanganisa i-antibody, yakha inkimbinkimbi entsha. Ulayini obomvu yizinga elivela endaweni yokulawula ikhwalithi (C) ukuze kuhlungwe ukuthi akhona yini amasampula anele nokuthi inqubo ye-chromatography ijwayelekile yini. Iphinde isetshenziswe njengezinga lokulawula langaphakathi lama-reagents.
Inqubo Yokuhlola
Inqubo yokuhlola ye-WIZ-A202 kanye ne-Portable Immune analyzer WIZ-A101 ibhekisela kumyalelo womyalelo. Inqubo yokuhlola ebonakalayo imi kanje:
1. Khipha ikhadi lokuhlola esikhwameni se-foil, ulibeke etafuleni lezinga bese ulimaka.
2. Engeza i-serum engu-10μl noma isampula ye-plasma noma isampula legazi eliphelele lama-20ul ukuze usampula kahle ekhadini nge-dispette enikeziwe, bese wengeza u-100μl (cishe amaconsi angu-2-3) isampula ye-diluent; qala isikhathi.
3. Linda okungenani imizuzu engu-10-15 futhi ufunde umphumela, umphumela awuvumelekile ngemva kwemizuzu engu-15.
Mayelana NATHI
I-Xiamen Baysen Medical Tech limited iyibhizinisi eliphakeme lebhayoloji elizinikela ekufakweni kwe-reagent yokuxilonga esheshayo futhi ehlanganisa ucwaningo nentuthuko, ukukhiqiza nokudayisa kukonke. Kunezisebenzi eziningi ezithuthukile zocwaningo nabaphathi bezokuthengisa enkampanini, bonke banokuhlangenwe nakho okucebile kokusebenza e-china kanye nebhizinisi lamazwe ngamazwe le-biopharmaceutical.