Isimiso

Ulwelwesi lwedivayisi yokuhlola luhlanganiswe ne-antibod ye-TF esifundeni sokuhlola kanye nembuzi anti Unogwaja I-IGG antibody kwisifunda sokulawula. I-Lable Pad ihlanganiswe yi-fluorescence ilebuli i-anti tf antibody kanye ne-rabbit igg kusengaphambili. Lapho uhlola isampula elimuhle, i-TF Antigen yesampula ihlangana ne-fluorescence ilebula antiilody antibod, bese wenza umxube wokuzivikela. Ngaphansi kwesenzo se-Immunochrotography, ukugeleza okuyinkimbinkimbi kokuqondiswa kwephepha lokumunca. Lapho kuyinkimbinkimbi edlula esifundeni sokuhlola, kuhlanganiswe ne-anti TF coating antiating antibody, amafomu amasha ayinkimbinkimbi.

Uma kungenamibi, isampula ayiqukethe ukudluliselwa noma okuqukethwe kuphansi, ukuze inhlanganisiwe yokuzivikela ingekho. Ngeke kube khona umugqa obomvu endaweni yokutholwa (T). Akunandaba noma ngabe i-antimwody ye-TF ikhona ku-specimen noma cha, i-colloidal esele yegolide enelembu lokulwa ne-anti-human monoclonal antilody ne-IGG antibody ehlanganiswe endaweni yokulawula ikhwalithi (C). Lapho-ke ama-agglutitates athuthukisa umbala endaweni yokulawula ikhwalithi, futhi ulayini obomvu uzovela (c). Umugqa obomvu yilezi zindlela ezivela endaweni yokulawula ikhwalithi (C) yokwahlulela ukuthi ngabe kukhona amasampula anele nokuthi inqubo ye-chromatomatography ijwayelekile. Iphinde isetshenziswe njengezinga lokulawula langaphakathi lama-reagents.

Inqubo yokuhlola

Sicela ufunde ukufaka iphakethe ngaphambi kokuhlolwa.

1. Lawula ikhadi lokuhlola kusuka esikhwameni se-foil, ulibeke etafuleni lezinga bese umaka.

2.Remove cap kusuka kushubhu eyisampula futhi ulahle amasampula amabili okuqala ahlanjululwe, engeza amaconsi ama-3 (cishe u-100Ul) Ayikho Isampula evuswe nge-Bubble ngokuqondile futhi kancane kancane ekhadini elinikezwe ngekhadi elinikeziwe. Bese uqala isibali sikhathi.