Isimiso

Ulwelwesi lwedivayisi yokuhlola luhlanganiswe ne-rotavirus group a antigen esifundeni sokuhlola kanye nembuzi anti unogwaja IGG antibody on the control. I-Lable Pad ihlanganiswe yi-fluorescence ilebula anti rotavirus group a no unogwaja iGG kusengaphambili. Lapho uhlola isampula elinethemba, i-RV in isampula ihlangana ne-fluorescence ilebula anti rotavirus group a, bese wenza umxube wokuzivikela. Ngaphansi kwesenzo se-Immunochrotography, ukugeleza okuyinkimbinkimbi kokuqondiswa kwephepha lokumunca. Lapho kuyinkimbinkimbi edlula esifundeni sokuhlola, kuhlanganiswe neqembu le-anti-rotavirus i-antibody, amafomu amasha. Uma kungenamibi, alikho iqembu le-rotavirus elithi antigen kusampula, ukuze kube lula ukuzivikela komzimba, ngeke kube nomugqa obomvu endaweni yokutholwa (T). Kungakhathalekile ukuthi iqembu i-rotavirus likhona yini ku-specimen, i-latex-laled IGG yegundane ifakwe kwi-Chromatography endaweni yokulawula ikhwalithi (c) futhi ithunjwe yimbuzi anti-mouse iGG antilody. Kuzovela umugqa obomvu endaweni yokulawula ikhwalithi (C). Umugqa obomvu yilezi zindlela ezivela endaweni yokulawula ikhwalithi (C) yokwahlulela ukuthi ngabe kukhona amasampula anele nokuthi inqubo ye-chromatomatography ijwayelekile. Iphinde isetshenziswe njengezinga lokulawula langaphakathi lama-reagents.

Inqubo yokuhlola:

Iziguli ezi-1.Symptomatic kufanele ziqoqwe. Ngokusho kwemibiko, ukukhishwa okuphezulu kwe-rotavirus kumalondolo eziguli ezine-gastroenteritis kwenzeka izinsuku ezingama-3-5 ngemuva kokuqala kwalesi sifo nezinsuku ezingama-3-13 ngemuva kokuqala kwezimpawu. Uma isampula liqoqwa isikhathi eside ngemuva kohudo, inani lama-antigen kungenzeka lingabi ngokwanele ukuba senzeke ukusabela okuhle.

Amasampula kufanele aqoqwe esitsheni esihlanzekile, esomile, esingenamanzi esingenakuhlanza izihlanzi kanye nezindawo zokugcina.

3.I-don-diarrhea iziguli, amasampula aqoqiwe ama-Faces akufanele abe ngaphansi kwamagremu ayi-1-2. Ezigulini ezinesifo sohudo, uma indle i-liquid, sicela uqoqe okungenani i-1-2 ml yama-Faece ketshezi. Uma indle iqukethe igazi eliningi ne-mucus, sicela uqoqe isampula futhi.

I-4.Itulwa ukuvivinya amasampula ngokushesha ngemuva kokuqoqwa, ngaphandle kwalokho kufanele ithunyelwe elabhorethri kungakapheli amahora ayi-6 futhi igcinwe ku-2-8 ° C. Uma amasampula engahloliwe kungakapheli amahora angama-72, kufanele agcinwe emazingeni okushisa angezansi -15 ° C.

5.Sebenzisa ama-feece amasha wokuhlola, namasampula we-Faeces ahlanganiswe namanzi angcolile noma ancishisiwe