ISIMISO

Umucu une-anti cal coating McAb endaweni yokuhlola kanye ne-anti-rabbit IgG yamasosha omzimba endaweni yokulawula, eboshelwe ku-membrane chromatography kusengaphambili. Iphedi elinelebula limbozwe ngegolide eli-colloidal elibhalwe ukuthi i-anti cal McAb kanye ne-colloidal yegolide elibhalwe ukuthi i-rabbit IgG antibody kusengaphambili. Lapho kuhlolwa isampula ephozithivu, i-cal kusampula ihlanganiswe negolide eli-colloidal elibhalwe ukuthi i-anti cal McAb, futhi yakhe i-immune complex, njengoba ivunyelwe ukufuduka eduze komugqa wokuhlola, inkimbinkimbi ye-cal conjugate ithwetshulwa yi-anti cal coating McAb kulwelwesi kanye nesimo. “I-anti cal coating McAb-cal-colloidal gold ebhalwe ukuthi anti cal McAb” inkimbinkimbi, ibhande lokuhlola elinemibala livele endaweni yokuhlola. Ukuqina kombala kuhlotshaniswa kahle nokuqukethwe kwekhalori. Isampula elibi alikhiqizi ibhendi yokuhlola ngenxa yokungabikho kwe-colloidal gold conjugate cal complex. Akunandaba ukuthi i-cal ikhona kusampula noma cha, kunomugqa obomvu ovela endaweni eyireferensi nendawo yokulawula ikhwalithi, ethathwa njengamazinga ebhizinisi angaphakathi ekhwalithi.

Inqubo Yokuhlola:

1.Khipha udondolo lwesampula, ulufake esampuleni yobulongwe, bese ubuyisela induku yesampula, uyiqinise bese uyinyakazisa kahle, phinda isenzo izikhathi ezi-3. Noma usebenzisa isampula yodondolo ukhethe isampula yobulongwe obungaba ngu-50mg, bese ufaka ishubhu lesampula lobulongwe eliqukethe isampula lokuhlanjululwa, bese ugoqa uqinise.

2. Sebenzisa amasampula e-pipette alahlwayo thatha isampula yendle elincanyana esigulini sohudo, bese wengeza amathonsi ama-3 (cishe 100uL) eshubhuni lokusampula le-fecal bese unyakazisa kahle, ubeke eceleni.

3.Khipha ikhadi lokuhlola esikhwameni se-foil, ulibeke etafuleni lezinga bese ulimaka.

4.Khipha ikepisi kushubhu yesampula bese ulahla amathonsi amabili okuqala esampula ehlanjululiwe, engeza amaconsi angu-4 (cishe u-100uL) alikho ibhamuza elihlanjululwe isampula lime mpo futhi kancane ungene emthonjeni wesampula wekhadi nge-dispette enikeziwe, qala isikhathi.

5.Umphumela kufanele ufundwe phakathi kwemizuzu eyi-10-15, futhi awuvumelekile ngemuva kwemizuzu eyi-15.