Isimiso

Ulwelwesi lwedivayisi yokuhlola luhlanganiswe neMicroalbumin Antigen esifundeni sokuhlola kanye nembuzi anti Unogwaja I-IGG Antibody on the Control Region. I-Lable Pad ihlanganiswe yi-fluorescence ilebula microalbumin kanye noRabbit Igg kusengaphambili. Uma kungekho albhamuin emchameni, i-colloidal yegolide efakwe amalebula we-monoclonal antilody e-colloclonal ekhasini le-colloidal ephepheni elinomchamo oya emgqeni wokutholwa, futhi ahlanganise ne-antigen eboshwe nge-Albic umugqa. Futhi umbala womugqa ube mnyama kunombala womugqa endaweni yokulawula (C), lo ngumphumela omubi. Uma umchamo uqukethe i-albhamuin, azoncintisana ne-antigen enama-albito ehlanganiswe nolwelwesi ukubopha amasayithi anomkhawulo we-antibod ku-colloidal yegolide e-anti-alebula antibonal antilody. Njengoba inani le-albamin emchameni liyanda, lihlola

Umbala womugqa uzoba lula futhi ulula. Okuqukethwe kwe-albhamuin kumchamo kungatholwa ngobuningi ngokuqhathanisa indawo ye-Detection (T) nendawo yokulawula (c). Indawo yokulawula ikhwalithi (C) kanye ne-Reference Area (R) kwikhithi izohlala ivela ngesikhathi sokuhlolwa, futhi ungabi nalutho nokuba khona komchamo we-albhamuin. Indawo yokulawula (C) kanye nereferensi yendawo (R) ingasetshenziswa njengenkomba yezethenjwa yekhwalithi yangaphakathi yekhithi.

Inqubo yokuhlola:

Uyacelwa ukuthi ufunde incwadi yokusebenza kwensimbi yokusebenza kwensimbi kanye nokufaka iphakheji ngaphambi kokuhlolwa. Amasampula aphansi wokushisa kwegumbi ngaphambi kokusebenzisa.

1.Thola ikhadi lokuhlola esikhwameni se-foil. Yibeke phansi endaweni evundlile kanye noMark.

2.Thola isampula yomchamo nge-pipette elahlekile, lahla amaconsi amabili okuqala wesampula yomchamo. Faka amaconsi ama-3 (cishe i-100Ul) yomchamo wama-bubble-free enkabeni yekhaza eyisampula yekhadi lokuhlola elibheke phezulu bese uqala isikhathi.

3.Funda umphumela ngemizuzu engu-10-16. Okungalungile uma kungaphezulu kwemizuzu eyi-15.