ISIMISO

I-membrane yedivayisi yokuhlola imbozwe nge-anti AFP antibody endaweni yokuhlola kanye ne-anti-rabbit IgG antibody yembuzi endaweni yokulawula. Iphedi elebulayo limbozwe nge-fluorescence ebhalwe ukuthi i-anti AFP antibody kanye ne-IgG yonogwaja kusengaphambili. Lapho kuhlolwa isampula elihle, i-antigen ye-AFP kusampula ihlangana ne-fluorescence ebhalwe ukuthi i-anti AFP antibody, bese yenza ingxube yokuzivikela komzimba. Ngaphansi kwesenzo se-immunochromatography, ukugeleza okuyinkimbinkimbi ekuqondeni kwephepha elimuncayo, lapho inkimbinkimbi idlula isifunda sokuhlola, ihlangene ne-anti AFP yokuhlanganisa i-antibody, yakha inkimbinkimbi entsha.Izinga le-AFP lihlotshaniswa kahle nesignali ye-fluorescence, kanye nokuhlushwa kwe-AFP. kusampula kungatholwa nge-fluorescence immunoassay assay.

Inqubo Yokuhlola

Sicela ufunde imanuwali yokusebenza kwezinsimbi kanye nokufakwa kwephakheji ngaphambi kokuhlola.

1. Beka eceleni wonke ama-reagents namasampuli ekamelweni lokushisa.
2. Vula i-Portable Immune Analyzer(WIZ-A101), faka ukungena ngemvume kwephasiwedi ye-akhawunti ngokwendlela yokusebenza yensimbi, bese ufaka isixhumi esibonakalayo sokubona.
3. Skena ikhodi yokuhlonza ukuze uqinisekise into yokuhlola.
4. Khipha ikhadi lokuhlola esikhwameni se-foil.
5. Faka ikhadi lokuhlola endaweni yekhadi, skena ikhodi ye-QR, bese unquma into yokuhlola.
6. Engeza i-serum engu-20μL noma isampula ye-plasma ku-diluent eyisampula, bese uxuba kahle.
7. Engeza isisombululo sesampula esingu-80μL ukuze uthole isampula yomthombo wekhadi.
8. Chofoza inkinobho ethi "ukuhlolwa okujwayelekile", ngemva kwemizuzu engu-15, ithuluzi lizothola ngokuzenzakalelayo ikhadi lokuhlola, lingakwazi ukufunda imiphumela esikrinini sokubonisa sensimbi, futhi lirekhode/liphrinte imiphumela yokuhlolwa.
9. Bheka imiyalelo ye-Portable Immune Analyzer(WIZ-A101).