Isimiso

Ulwelwesi lwedivayisi yokuhlola luhlanganiswe ne-anti AFP antibody kwisifunda sokuhlola kanye nembuzi anti Unogwaja IGG Antibody on the control. I-Lable Pad ihlanganiswe yi-fluorescence ilebula anti antp antibody kanye ne-rabbit igg kusengaphambili. Lapho uhlola isampula elinethemba, i-antigen ye-AFP in isampula ihlangana ne-fluorescence ilebula anti antibod antibod, bese wenza umxube wokuzivikela. Ngaphansi kwesenzo se-Immunochrotography, ukugeleza okuyinkimbinkimbi kokuqondisa kwephepha lokungena, lapho kudluliswe isikhathi sokuhlola, kuhlanganiswe ne-anti afp coating antilod, amafomu amasha acwebile.AfP axhumeka kahle ngesiginali ye-fluorescence, kanye ne-afp Kusampula kungatholwa yi-fluorencem immunoassay assay.

Inqubo yokuhlola

Uyacelwa ukuthi ufunde incwadi yokusebenza kwensimbi yokusebenza kwensimbi kanye nokufaka iphakheji ngaphambi kokuhlolwa.

1. Beka eceleni yonke imiphenduli namasampula wokushisa kwegumbi.
2. Vula i-Immune Analyzer ye-Immunezer (Wiz-A101), faka ukungena kwephasiwedi ye-akhawunti ngokuya ngendlela yokusebenza yensimbi, bese ufaka isikhombimsebenzisi sokuthola.
3. Skena ikhodi yamazinyo ukuze uqinisekise into yokuhlola.
4. Thatha ikhadi lokuhlola esikhwameni se-foil.
5 Faka ikhadi lokuhlola ku-slot yekhadi, scan ikhodi ye-QR, bese unquma into yokuhlola.
6. Faka ama-20μl serum noma isampula ye-plasma kwi-dilount esampule, uhlanganise kahle ..
7. Faka isixazululo sesampuli esingu-80μL ukuze isampula kahle ekhadini.
8. Chofoza inkinobho ethi "Ukuhlolwa Okujwayelekile", ngemuva kwemizuzu engu-15, ithuluzi lizobona ngokuzenzakalelayo ikhadi lokuhlola, lingafunda imiphumela kusuka esikrinini sokubonisa sensimbi, bese uqopha / phrinta imiphumela yokuhlola.
9. Bheka imiyalo ye-Immune Analyzer ephathekayo (Wiz-A101).