Sijabulela isimo esihle kakhulu phakathi kwamathemba ethu ekhwalithi yethu enkulu yokuthengisa, intengo yokuncintisana kanye nensizakalo efanelekile yeminyaka engu-8 ethumela ngaphandle iChina inembileUkuhlolwa okusheshayo kwe-HCVIzinsizakusebenza zokuhlola okusheshayo ezindaweni zokuhlaziywa kwe-pathological, sesizethemba ukuthi kuzothathwa njengokuthi kuzothathwa njengokuthembisa okuzayo futhi sethemba ukuthi singabambisana nesikhathi eside ngamathemba avela kuyo yonke imvelo evela kuyo yonke imvelo.
Sijabulela isimo esihle kakhulu phakathi kwamathemba ethu ekhwalithi yethu enkulu yokuthengisa ephezulu, intengo yokuncintisana kanye nensizakalo efanelekayo yeChina ngokushesha ukuhlolwa, Ukuhlolwa okusheshayo kwe-HCV, Izimpahla zethu zithandwa kakhulu eZwini, njengeNingizimu Melika, i-Afrika, i-Asia nokunye. Izinkampani "zokwakha imikhiqizo esezingeni lokuqala" njengenhloso, futhi zilwele ukunikela amakhasimende ngezixazululo ezisezingeni eliphakeme, zinikezela ngekhwalithi ephezulu yokuthengisa ngemuva kokusekelwa kwezobuchwepheshe kanye nokuxhaswa kwezobuchwepheshe, ukuzuza umsebenzi oncono kanye nekusasa!
Ngokusetshenziswa kokuxilongwa kwe-vitro kuphela

Uyacelwa ukuthi ufunde leli phakethe Faka ngokucophelela ngaphambi kokusebenzisa futhi ulandele imiyalo ngokuqinile. Ukwethembeka kwemiphumela ye-Assay akunakuqinisekiswa uma kukhona ukuphambuka emiyalweni ekuleW kufakiwe.

Ukusetshenziswa okuhlosiwe

I-Diagnostic Kit ye-Hepatitis C Virus antibody (Fluorencence Immunochrohamaygraphic Assay is a Fluorescence Immunochrographic Assay for the antim antim noma i-plasma, okuyinani elibalulekile lokuthola isampula nge-hepatitis C.All fax izindlela. Lokhu kuhlola kuhloselwe ukusetshenziswa kwezempilo okukhethekile kuphela

1.Lay ubeka wonke ama-reagents namasampula wokushisa kwegumbi.
I-2.open them immune analyzer (Wiz-A101), faka ukungena ngemvume kwe-akhawunti ngokusho kwendlela yokusebenza yensimbi, bese ufaka isikhombimsebenzisi sokuthola.
I-3.Sc Ikhodi Yokucacisa Ukuqinisekisa Into Yokuhlola.
4.Thola ikhadi lokuhlola esikhwameni se-foil.
5.Shuma ikhadi lokuhlola ekhadini lekhadi, scan ikhodi ye-QR, bese unquma into yokuhlola.
I-6.Add 20μL serum noma isampula ye-plasma ukuze isampula isampula, uhlanganise kahle ..
7.ADD 80μL isixazululo isampula yesampula kahle yekhadi.
8.Chofoza inkinobho ethi "Ukuhlolwa okujwayelekile", ngemuva kwemizuzu eyi-15, ithuluzi lizobona ngokuzenzakalelayo ikhadi lokuhlola, lingafunda imiphumela kusuka esikrinini sokubonisa sensimbi, bese uqopha / phrinta imiphumela yokuhlola.
9.Refer ekufundisweni kwe-immune analyzer (Wiz-A101).

Ukubeka kafushane

I-Hepatitis C Virus (HCV) iyimvilophu, i-RNA eyodwa eyakhiwe kahle i-RNA (9.5 KB) igciwane lomndeni we-flaviviridae. Ama-genotypes amakhulu ayisithupha nochungechunge lwama-subtypes atholakele. Ihlukaniswe ngo-1989, i-HCV manje ibonwa njengembangela enkulu ye-Throntfusion ehambisana ne-non-a, non-B Hepatitis. Lesi sifo sibonakaliswa ngefomu elibi futhi elingamahlalakhona. Bangaphezu kuka-50% abantu abanegciwane ababa nzima, impilo esongela i-hepatitis engalapheki enama-cirrhosis wesibindi nama-hepatocellular carcinomas. Kusukela isingeniso ngo-1990 sokuhlolwa kokulwa kwegazi kweminikelo yegazi, izehlakalo zalokhu kutheleleka kubamukeli be-transfusion bancishisiwe kakhulu. Ucwaningo lweMitholampilo lukhombisa ukuthi inani elibalulekile labantu abanegciwane le-HCV bathuthukisa amasosha omzimba ku-NS5 angelona amaprotheni wegciwane. Kulokhu, izivivinyo zifaka ama-antigen kusuka esifundeni se-NS5 se-genome yegciwane ngaphezu kwe-NS3 (C200), NS4 (C22) kanye ne-Core (C22).

Umgomo wenqubo

Ulwelwesi lwedivaysi yokuhlola luhlanganiswe ne-HCV Antigen esifundeni sokuhlola kanye nembuzi anti Unogwaja IGG Antibody on the Control Region. I-Lable Pad ihlanganiswe yi-fluorescence ilebula i-HCV Antigen ne-Igg Rabbit kusengaphambili. Lapho uhlola isampula elinethemba, i-antimv ye-HCV in isampula hlanganisa ne-fluorescence ilebula i-HCV Antigen, bese wenza ingxube yobusuku bokuzivikela. Ngaphansi kwesenzo se-Immunochrotography, ukugeleza okuyinkimbinkimbi kokuqondisa kwephepha lokumunca, lapho kudlule isikhathi sokuhlola, kuhlanganiswe ne-HCV antigen coatigen antigen, amafomu amasha acwebile.hcv antibody axhumeka kahle nesiginali ye-fluorescence, kanye nokuhlushwa kwe I-antibody ye-HCV in isampula ingatholwa yi-fluorencem immunoassay assay

Ama-reagents nezinto zokwenziwa

25T Iphakethe Izakhi:
.Test Card ngakunye icwebezela nge-desiccant
.SAMPLE DLOLUENTES
.Fackage Faka

Izinto ezidingekayo kodwa azinikeziwe
Isithakongo esiqoqiwe sesampula, isibali sikhathi
Ukuqoqwa kwesampula nesitoreji
1.Izimpumu ezihlolwe zingaba yi-serum, i-heparin anticoaagulant plasma noma i-edta anticoaagulant plasma.
2.Faka kumasu ajwayelekile ukuqoqa isampula. Isampula ye-serum noma ye-plasma ingagcinwa esiqandisini ku-2-8 ℃ ngezinsuku eziyi-7 days
I-3.all isampula igweme imijikelezo ye-freeze-thaw.

Inqubo yokufa
Uyacelwa ukuthi ufunde incwadi yokusebenza kwensimbi yokusebenza kwensimbi kanye nokufaka iphakheji ngaphambi kokuhlolwa.
Umphumela wokuhlola we-.Lokhuwo we-. .
.Lokhu i-realent isetshenziselwa kuphela ukuhlolwa kwe-serum ne-plasma. Kungase kungatholi umphumela onembile uma usetshenziselwa amanye amasampula anjenge-saliva nomchamo nokunye.

Izici Zokusebenza

Ukunqubekela phambili
1.post transfusion hepatitis. Ku: Moore SB, ed. Izifo zegciwane ezidluliselwe empontshelwe. U-Alington, VA. Ngingu. Ukuhlanganisa. Amabhange egazi, kk. 53-38.
2.Hansen jh, et al.hama ukuphazanyiswa nge-murine monoclonal antibody-based immunody [j] .j we-Clin immunoassay, 1993,16: 294-299.
I-3.Levinson SS.Imvelo yama-antibophilies we-hetorophilic neqhaza ekuphazanyisweni kwe-immunoassay [J] .j we-Clin immunoassay, 1992,15: 108-114.
I-4.Alter HJ., Purcell RH, Holland PV, et al. (1978) Umenzeli ohambisanayo ku-non-a hepatitis. Lancet I: 459-463.
I-5.Choo QL, i-Weiner AJ, Overby Aj, Overby AJ, Kuo G, HourOn Courson M. (1990) Hepatitis M. (1990) Hepatitis M. (1990) Igciwane le-Hepatitis C. (1990) Igciwane le-Hepatitis C. Br Med Bull 46: 423-441.
6.Engvall E, Perlmann P. (1971) enzyme exhumanisa i-Imunosorbent Assay (ELISA): I-Assay ye-Assay ye-IGG. I-Imunochemistry 8: 871-874.

Amanani alindelwe

I-HCV-AB <0.02

Kunconywa ukuthi ilebhu ngalinye ngalinye lasungulwa uhla lwalo olujwayelekile olumele abantu balo.

Imiphumela yokuhlola nokutolika

• Le datha engenhla ingumphumela wokuhlolwa kwe-HCV-AB reagent, futhi kuphakanyiswa ukuthi ilabhorethri ngalinye kufanele lisungule amanani ahlukahlukene we-HCV-AB alungele inani labantu kulesi sifunda. Imiphumela engenhla ingeyokubhekiswa kuphela.
• Imiphumela yale ndlela isebenza kuphela kumabanga okuthola amareferensi asungulwe kule ndlela, futhi akukho ukuqhathanisa okuqondile nezinye izindlela.
• Ezinye izinto nazo zingadala amaphutha emiphumeleni yokuthola, kufaka phakathi izizathu zobuchwepheshe, amaphutha okusebenza nezinye izinto zesampula.

Ukugcinwa nokuqina

1. Ikhithi iyizinyanga eziyi-18 zeShelf-Life kusukela ngosuku lokukhiqiza. Gcina amakhithi angasetshenziswanga ngo-2-30 ° C. Ungabazi. Ungasebenzisi ngaphezu kosuku lokuphelelwa yisikhathi.
2. Ungavuli isikhwama esivaliwe uze ulungele ukwenza isivivinyo, futhi ukuhlolwa okukodwa kuphakanyiswa ukuthi kusetshenziswe ngaphansi kwendawo edingekayo (lokushisa 2-35 ℃, umswakama u-40-90%) ngaphakathi kwamaminithi angama-60 ngokushesha okukhulu ngangokunokwenzeka .
3. Isampula i-dilount isetshenziswa ngokushesha ngemuva kokuvulwa.

Izexwayiso kanye nokuqapha
.Ikhithi kufanele ivalwe futhi ivikelwe kumswakama.
.Imininingwane emihle izoqinisekiswa ezinye izindlela.
.Zonke izinhlobo zizophathwa njengokungcoliswa okungaba khona.
.Musa ukusebenzisa i-reagent ephelelwe yisikhathi.
.Musa ukuguqula ama-reagents phakathi kwama-kits ane-Lot ehlukile Cha ..
.Musa ukusebenzisa kabusha amakhadi okuhlola nanoma yiziphi izesekeli ezilahlwayo.
.Ukuphamba, isampula ngokweqile noma elincane kungaholela ekuphumeni kwemiphumela.

LUkufanisa
.Njenganoma ikuphi ama-antibodies egundane eguqayo, kungenzeka ukuthi kuphazanyiswe ngama-antibodies abantu alwa nobuntu (Hama) ku-specimen. Izinhlobo ezivela ezigulini ezithole amalungiselelo ama-antibodies e-monoclonal yokuxilongwa noma ukwelashwa kungaqukatha uHama. Izinhlobo ezinjalo zingadala imiphumela emibi noma engamanga.
Ukhiye wezimpawu ezisetshenzisiwe:

	Kudivayisi yezokwelapha yokuxilongwa kwe-vitro
	Umenzi
	Gcina ngo-2-30 ℃
	Usuku lokuphelelwa isikhathi
	Ungaphinde usebenzise kabusha
	Isiqapheliso
	Bheka imiyalo yokusebenzisa