UMGAQO

Umtya une-anti cal coating iMcAb kwindawo yovavanyo kunye nebhokhwe yokuchasa imivundla ye-IgG kwingingqi yolawulo, ebotshelelwa kwi-membrane chromatography kwangaphambili. Iphedi elayibhile iqatywe ngegolide ecolloidal ebhalwe anti cal McAb kunye negolide eyicolloidal ebhalwe i-antibody yomvundla we-IgG kwangaphambili. Xa uvavanya isampulu eqinisekileyo, i-cal kwisampulu yesampulu edibene negolide ye-colloidal ebhalwe anti cal McAb, kwaye yenza i-immune complex, njengoko ivunyelwe ukuba ifudukele ecaleni komtya wovavanyo, i-cal conjugate complex ibanjwa yi-anti cal coating McAb kwi-membrane kunye nefom. I-“anti cal coating iMcAb-cal-colloidal gold ebhalwe anti cal McAb” complex, ibhendi yovavanyo enemibala yavela kwindawo yovavanyo. Ukuqina kombala kuhambelana kakuhle nomxholo we-cal. Isampuli engalunganga ayivelisi ibhendi yovavanyo ngenxa yokungabikho kwe-colloidal gold conjugate cal complex. Kungakhathaliseki ukuba i-cal ikhona kwisampulu okanye ayikho, kukho umgca obomvu ovela kummandla wereferensi kunye nommandla wolawulo lomgangatho, othathwa njengomgangatho wemigangatho yeshishini langaphakathi.

Inkqubo yoVavanyo:

1.Thatha intonga yesampulu, uyifake kwisampulu yelindle, emva koko ubeke intonga yesampulu ngasemva, jija uqinise kakuhle, phinda intshukumo ka-3. Okanye usebenzisa intonga yesampulu ichola malunga ne-50mg yesampuli yelindle, kwaye ufake kwityhubhu yesampulu yelindle equlethe isampulu yedilution, kwaye ujije ngokuqinileyo.

2. Sebenzisa isampulu yepipette elahlwayo thatha isampuli yelindle elicekeceke kakhulu kumguli worhudo, emva koko wongeze amathontsi ama-3 (malunga ne-100uL) kwityhubhu yesampulu ye-fecal kwaye uvuthulule kakuhle, ubeke ecaleni.

3.Khupha ikhadi lovavanyo kwibhegi yefoyile, uyibeke kwitafile yenqanaba kwaye uyiphawule.

4.Susa i-cap kwi-tube yesampuli kwaye ulahle i-drops yokuqala ye-diluted samples, yongeza i-4.drops (malunga ne-100uL) akukho bubble diluted isampuli ngokuthe nkqo kwaye ngokucotha kwisampulu kakuhle yekhadi kunye ne-dispette enikeziweyo, qalisa ixesha.

5.Isiphumo kufuneka sifundwe kwimizuzu eyi-10-15, kwaye ayisebenzi emva kwemizuzu eli-15.