Le khithi yoVavanyo ifanelekile ukufumanisa ubungakanani behomoni ye-adrenocorticotropic (ATCH) kwisampulu yePlasma yoMntu kwi-Vitro, esetyenziselwa ikakhulu ukuxilongwa kwe-ACTH hypersecretion, i-ACTH ezimeleyo evelisa izicubu ze-pituitary hypopituitarism kunye nokunqongophala kwe-ACTH kunye ne-ectopic test syndrome kufuneka isiphumo sovavanyo lwe-ACTH. zihlalutywe zidityaniswe nolunye ulwazi lwezonyango .