Umgaqo

I-Strip ine-MP-AGIGE YAMANDLA EMVA KOKUGQIBELA NOKUGQIBELA I-Anti Mouse Antiy Anti yoLawulo, efakwe kwi-membrane chromatographys kwangaphambili. I-pad ibuthathaka idityaniswe yiColoidal yeGolide yeGolide ebhalwe i-mouse-anti yoMntu kwi-Igm ye-Igm ye-Igm yangaphambili. Xa uvavanya isampulu elungileyo, i-MP-i-Igm kwi-payini yesampulu ye-colloidal yegolide eneemouse-anti yoMntu we-Igm ye-Igm ye-Igm ye-Igm, kunye neForat. Phantsi kwesenzo se-i-Ignomamographromatographromatographromatographromatography, ubunzima kunye nesampulu ngaphakathi kwe-nitrocellulose ye-membrane kwindawo yovavanyo, xa kudityaniswa ne-Antign ye-Antigen, i-Antign-MP I-ANGM-Colloidal igolide ineemouse-anti yoMntu we-Antigm Mcab "I-Marants, ibhendi yovavanyo olunombala wavela kwingingqi yovavanyo. Isampulu engalunganga ayivelisi i-band ye-veres ngenxa yomanyano olunengxaki. Nokuba i-MP-I-Igm ikhoyo kwisampuli okanye hayi, kukho umtya obomvu kwingingqi yolawulo oluphezulu, ethathwa njengemigangatho yeshishini yangaphakathi.

Inkqubo yovavanyo:

Inkqubo yovavanyo lwe-Wiz-A101 yabona imiyalelo yohlaluko oluqhelekileyo lonyango. Inkqubo yovavanyo olubonakalayo ngolu hlobo lulandelayo:

1. Beka ecaleni zonke ii-regentrs kunye neesampulu kubushushu begumbi.
2. Khupha ikhadi lovavanyo kwingxowa ye-foil, yibeke etafileni kwaye uyiphawule.
3. Yongeza i-Serum okanye isampulu yeplasma okanye i-20μl isampulu yegazi iphela kwi-SEAMICE, i-100 ye-2sl) isampulu yesampulu, qala ixesha.
4. Lindela ubuncinci imizuzu eli-10-15 kwaye ufunde iziphumo, isiphumo asisebenzi emva kwemizuzu eli-15.