UMGAQO

I-membrane yesixhobo sokuvavanya ifakwe kunye neQela A kunye ne-adenovirus ye-antigen kummandla wovavanyo kunye nebhokhwe yokulwa nomvundla we-IgG kwingingqi yokulawula. Iphedi elayibhile iqatywe nge-fluorescence ebhalwe anti Group A kunye ne-adenovirus kunye nomvundla IgG kwangaphambili. Xa uvavanyo lwesampulu eqinisekileyo yeQela A kunye ne-adenovirus, iQela A kunye ne-adenovirus kwisampulu zidibanisa ne-fluorescence ebhalwe i-anti Rotavirus Group A kunye ne-adenovirus, kwaye yenze umxube wokuzivikela komzimba. Ngaphantsi kwesenzo se-immunochromatography, ukuhamba okuyinkimbinkimbi kwicala lephepha elithathayo. Xa i-complex iphumelele ummandla wovavanyo, idityaniswe ne-anti-Rotavirus yeQela A kunye ne-adenovirus yokugquma i-antibody, yenza into entsha. Ukuba i-negative, ayikho i-Rotavirus Group A kunye ne-adenovirus antigen kwisampuli, ukwenzela ukuba ii-immune complexes zingenakwenziwa, akuyi kubakho umgca obomvu kwindawo yokufumanisa (T). Nokuba ngaba i-rotavirus yeqela kunye ne-adenovirus ikhona kwi-specimen, i-mouse ebhalwe nge-latex i-IgG i-chromatographed ukuya kwindawo yokulawula umgangatho (C) kwaye ibanjwe yibhokhwe ye-anti-mouse IgG antibody. Umgca obomvu uya kubonakala kwindawo yokulawula umgangatho (C). Umgca obomvu ngumgangatho ovela kwindawo yokulawula umgangatho (C) ukugweba ukuba kukho iisampuli ezaneleyo kunye nokuba inkqubo yechromatography iyinto eqhelekileyo. Ikwasetyenziswa njengomgangatho wolawulo lwangaphakathi kwii-reagents.

Inkqubo yoVavanyo:

1. Vula i-cap ye-tube yokuqokelela isampuli. Musa ukuchitha isisombululo kwibhotile.
2. Khupha intonga yesampulu, ifakwe kwisampulu yelindle (okanye sebenzisa intonga yesampulu ukukhetha malunga ne-50mg yelindle), emva koko ubuyisele intonga yesampulu, ujike uqinise kwaye ushukumise kakuhle, phinda isenzo izihlandlo ezi-3. Thatha inxalenye eyahlukileyo yesampulu yelindle ngexesha ngalinye. Emva kwesampulu, faka intonga yesampulu kwityhubhu yokuqokelela ilindle equlethe isampulu yokuxutywa, kwaye ujije idropha ngokuqinileyo. Ukuba ilindle lomguli onesifo sorhudo libhityile, iindiza zeplastiki ezilahlwayo zinokusetyenziselwa ukwenza isampulu. Ukusebenzisa isampulu yepipette elahlwayo thatha isampuli yelindle elicekeceke kakhulu kwisigulane sorhudo, uze wongeze amathontsi ama-3 (malunga ne-100uL) kwityhubhu yesampulu yelindle.
3. Vuthulula kakuhle isampuli kwaye ususe ikepusi kwincam yedropha kwaye uyibeke ecaleni.
4. Xa igcinwe kwiqondo lokushisa eliphantsi, ikiti kufuneka ibuyiselwe kwiqondo lokushisa ngaphambi kokusetyenziswa. Khupha ikhadi lovavanyo kwibhegi yefoyile, uyibeke kwitafile ekwinqanaba kwaye uyiphawule.
5. Susa i-cap kwi-tube yesampuli kwaye ulahle amaconsi amabini okuqala isampuli ehlanjululweyo, yongeza i-3 yehla (malunga ne-100uL) akukho bubble diluted isampuli ngokuthe nkqo kwaye ngokucotha kwisampulu kakuhle yekhadi kunye ne-dispette enikeziweyo, qalisa ixesha.
6. Isiphumo kufuneka sifundwe kwimizuzu eyi-10-15, kwaye ayisebenzi emva kwemizuzu eyi-15.