Isishwankathelo

IVitamin D yivithamini kwaye ikwayihomoni ye-steroid, ikakhulu kubandakanya i-VD2 kunye ne-VD3, eyakhiwayo ifana kakhulu. IVitamin D3 kwaye i-D2 iguqulwe ibe yi-25 hydroxyl vithamin d (kubandakanya i-25-Dihydroxyl vithamin D3 kunye d2). 25- (oh) i-VD emzimbeni womntu, ukwakhiwa kwezinzile, uxinzelelo oluphezulu. 25- (oh) I-VD ibonakalisa inani lilonke leVitamin D, kunye ne-VICON D, ukuze i-25- (o) i-VD ithathwa njengeyona nto iphambili ekuvavanyweni kwe-vitamin D.I i-ITMOCROPROGRAGRAGRAGRAGY kwaye inokunika isiphumo ngaphakathi kwemizuzu eli-15.

Umgaqo wenkqubo

I-membrane yesixhobo sovavanyo idityaniswe ne-Congemate ye-BSA kunye ne-25- (o) i-VD kwingingqi yovavanyo kunye nebhokhwe yebhokhwe yebhokhwe ye-antibibit igwebu. I-padker pad idityaniswe yi-Fluorescence Mark Anti 25- (oh) i-vd anti) kunye ne-rabibit yeg ngangoko. Xa isampulu yovavanyo, i-25- (o) i-VD kwisampulu idibanisa i-fluorescence ephawulwe i-Anti 25- (oh) i-vd anti), kwaye ibe ngumxube womzimba. Phantsi kwesenzo semilinganiselo ye-igonocchiromatographromatographromatography, ukuhamba okuntsonkothileyo kwicala le-Ancrictal, xa i-strader igqithile kwi-25- (O) i-25- (o) i-VD ifumaneka ngeSampulsasasce I-Assay.